Browsing by Author "Aissaoui, Mohammed"

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Effect of exhaustion of olive pomace on cellulases production from Trichoderma reesei RUT C30 and identification of the enzyme complexes
(Springer, 2024) Boutiche, Malika; Bennacer, Amel; Aissaoui, Mohammed; Meziant, Leila; Saci, Fairouz; Siar, El-Hocine; Boulaouad, Ilyes; Bekrar, Amel; Fiala, Samah; Hemoui, Hadjer; Sahir-Halouane, Fatma
Olive industry generates large quantities of solid by-products such as olive pomace. The objective of present work is to study the effect of exhaustion (residual oil extraction) by acetone solvent on the improvement of cellulase production using olive pomace as substrate, and to identify the crude enzymatic extract by electrophoretic analysis. The production of cellulases was done by the solid fermentation process using Trichoderma reesei RUT C30 as cellulolytic fungus grown on olive pomace. Cellulolytic activity was assessed by the filter paper method (FPase). Electrophoretic analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed for the identification of enzymatic extract of T. reesei RUT C30. Chemical composition (lipids, proteins, sugars and ash) was significantly (P < 0.05) affected after olive pomace exhaustion. In general, exhaustion by acetone solvent increased the fiber fraction. Exhaustion improved the results of FPase activity compared to those obtained on untreated substrate, giving values of 2.63 IU/gds and 1.1 IU/gds, respectively. The results of SDS-PAGE analysis revealed that the crude enzymatic extract contains two different enzyme complexes including cellulases and xylanases.
Exploring the antifungal properties of tannin extracts from Olea europaea var. rougette leaves against Aspergillus spp.
(Springer, 2024) Bennacer, Amel; Sahir-Halouane, Fatma; Aitslimane-Aitkaki, Sabrina; Tihar-Benzina, Farida; Oukali, Zahia; Oliveira, Ivo Vaz; Rahmouni, Naima; Aissaoui, Mohammed; Mohankumar, Ramar
Due to significant health risks associated with chemical antifungal agents in food storage, safe and natural sources are now one of the most frequently demanded ingredient for food preservation. Byproducts from olive trees provide an affordable and sustainable source of bioactive chemicals. This study will examine the mycelial growth and germination-inhibitory properties of Olea europaea var. rougette leaves and characterize their phytochemical makeup.The phytochemical screening revealed the presence of polyphenolics compounds mainly tanins.The chemical composition was analyzed by gas chromatography mass spectrometry (GCMS). Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, potassium iodide and vanilline methods, respectively. The mycelial growth and germination inhibition properties were examined on soft wheat based solid and liquid media. Tannic extract (ethyl acetate extract) revealed highest amounts in polyphenols, hydrolysable tanins and condensed. GC–MS profile of tannic extract showed the presence of 26 bioactive molecules known for their antifungal potential, mainly: Guaiacol (0.12%), 2-methoxy-4-vinylphenol (1.52%), Syringol (0.18%), p-tyrosol (4.70%), homovanillyl alcohol (1.32%), 4-propylresorcinol (1.07%), β-camphor (0.17%), β-turmerone (1.57%) and 4-phenyl-quinolin-2-ol (6.58%). The tannic extract (ethyl acetate fraction) showed significant mycelial growth and germination inhibition activities in contrast to hydro-acetonic and dichloromethanic extracts. Furthermore, myelial growth inhibition showed more effectiveness in comparison to germination inhibition technique.The results suggested that byproducts of Olea europaea var. rougette leaves are a substantial natural source of bioactive compounds, especially tannins with potent antifungal characteristics in vitro which make it interesting to investigate its effectiveness in vivo.
Structural characterization of phytochemical content, antibacterial, and antifungal activities of Juglans regia L. leaves cultivated in Algeria
(Elsevier, 2022) Bennacer, Amel; Sahir-Halouane, Fatma; Aitslimane-Aitkaki, Sabrina; Oukali, Zahia; Oliveira, Ivo Vaz; Rahmouni, Naima; Aissaoui, Mohammed
Many microorganisms cause diseases to plants, animals and humans. Recently, natural compounds extracted from different medicinal and officinal plants have had a promising antimicrobial potential. In this present study, the antifungal and antibacterial activities of Juglans regia leaves cultivated in Algeria have been evaluated. The chemical composition was analyzed by GCMS spectroscopy. Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, Potassium iodide and vanilline methods respectively. The antibacterial activity was tested against five bacterial strains and the antifungal activity was tested against seven fungal strains. The GC-MS profile of ethyl acetate fraction (tannic extract) revealed the presence of many interesting biomolecules we note: Phenol (0.19%), gaiucol (0.16%), catechol (0.65%), 2-Methoxy-4-vinylphenol (1.77%), eugenol (0.78%), (E)-coniferol (0.66%), 1, 5-naphthalenediol (2.01%) and 5-hydroxy-1, 4-naphthalenedione or juglone (0.18%). The tannic extract (ethyl acetate fraction) presented a high antifungal activity on Aspergillus terreus, Aspergillus ochraceus and Aspergillus Brasilliensis ATCC 16404 with percentage of growth inhibition of 77,033 ± 3,395, 73,327 ± 3,851 and 71,850 ± 0,641 respectively for the highest concentration. It also showed an interesting antibacterial activity against Klebseilla pneumoniae ATCC 70063 with minimal inhibitory concentration of 3.125 μg/ml comparing to the hydro-acetonic extract(MIC value). Our finding provides for the first time the chemical profiling of this species grown in Algeria and also an efficient and targeted natural approach to the discovery of classes of antibacterial and antifungal compounds for various applications