Browsing by Author "Khiari, Ouiza"

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Anticancer and antiproliferative activities of Algerian Origanum majorana L.’s essential oil on PC-3 and SKBR3 cells
(Taylor and francis, 2024) Hafid, Nourehouda; Bouchenak, Ouahiba; Serttas, Riza; Bouhenna, Mustapha Mounir; Khiari, Ouiza; Oussaid, Sounia; Suat, Erdogan
Cancer is a prominent cause of death globally, with breast cancer and prostate cancer being among the most devastating types. Therefore, the available anticancer treatments have some drawbacks, like higher toxicity and limited bioavailability. Thus, this study aimed to investigate for the first time the anticancer activity of Algerian Origanum majorana L.’s essential oil (OMEO). This research assessed the chemical profile of Algerian OMEO by gas chromatography coupled with mass spectrometry (GC-MS). The analysis revealed 29 compounds, which represent 98.08% of total volatile oil. The major compounds identified in OMEO were terpinen-4-ol (21.37%), γ-terpinene (15.78%), α-terpinene (10.43%), and trans-sabinene hydrate (9.27%). Additionally, MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide) was also used to test the cytotoxicity on prostate cancer (PC-3), breast cancer (SKBR3), and normal retinal pigment epithelium (ARPE-19) cell lines. The results showed a selective cytotoxicity effect by decreasing cell viability of PC-3 cancer cells with half inhibitory concentration (IC50) of 608.57 μg/mL and 672.5 μg/mL after 48h and 72h, respectively. Regarding SKBR3 cancer cells, the IC50 was 991.5 μg/mL. OMEO exhibited no significant cytotoxicity against normal (ARPE-19) cells. Furthermore, we conducted a cell apoptosis assay using Hochest 33342 dye to explore the potential mechanism pathway of OMEO. The findings verified that OMEO could trigger apoptosis in PC-3 and SBKR3 cancer cells. The ability of OMEO to inhibit cell migration assessed via wound healing assay revealed a significant decrease in cell migration. Our results imply that OMEO decreases cell viability by inducing cell apoptosis. Moreover, the oil suppresses cell migration in prostate cancer and breast cancer cells.
Ethnopharmacological survey of medicinal plant knowledge in boumerdes, algeria: traditional usage patterns and therapeutic applications
(Taylor &francis, 2025) Oussaid, Sounia; Bouchenak, Ouahibaa; Yahiaoui, Karima; Hafid, Nourehoudaa; Khiari, Ouiza; Khelef, Yahiac; Laoufi, Razikad
This ethnopharmacological survey documents the persistence of traditional plant knowledge in Boumerdes, Algeria. Through systematic interviews with 900 participants, quantitative analysis revealed distinct user profiles: predominantly women (52.1%) aged 50-70 years (39.0%), with inverse correlation to education level. Integration with conventional medicine was reported by 47% of users, motivated by perceived efficacy (41.5%) and safety (35.7%). Botanical inventory identified 263 species across 91 families, dominated by Lamiaceae (22.3%). Primary therapeutic applications targeted digestive (32.3%), respiratory (19.9%), and dermatological (11.5%) disorders. These findings validate the cultural resilience of traditional healthcare practices and underscore the imperative for phytochemical validation of documented species.
Etude préliminaire de la reproduction de la SaupeSarpasalpaLinnaeus, 1758 de la région algéroise
(2018-07-02) Khiari, Ouiza; Nettour, Khadidja; Aissat, F. prom.
L’étude préliminaire de la reproduction de la saupe SarpasalpaLinnaeus, 1758 de la région algéroise, a porté sur 46 spécimens (16,5 cm ˂ LT˂48,8 cm). L’échantillonnage a eu lieu au niveau des ports de Tamentfoust, Zemmouri et de Dellys entre le mois de Mars et le mois de Mai. Le sex-ratio est en faveur des hermaphrodites, avec absence totale des mâles. Le suivi mensuel du Rapport Gonado-Somatique (R.G.S.) et du Rapport Hépato-Somatique (R.H.S.) a montré que la période de ponte de cette espèce se situe probablement au mois d’Avril et révèlent que le foie contribue significativement dans la reproduction de la Saupe. Les relations biométriques indiquent une allométrie majorante supérieure à 1 entre la longueur totale (LT) et les longueurs, standard (LS), à la fourche (LF), la hauteur du corps (HC) et le diamètre de l’œil (DO). La relation taille-poids illustre une isométrie entre le poids total (WT) et la longueur totale (LT). L’étude histologique des gonades a révélé deux stades de maturité sexuelles chez les femelles, le stade II (début de maturité) et le stade V (Post-ponte).