Browsing by Author "Oliveira, Ivo Vaz"

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    Evaluation of the anti-inflammatory, antispasmodic and healing effects of walnut leaves juglans regia l. Aqueous extract
    (Moroccan Institute of Scientific and Technical Information, 2021) Bennacer, Amel; Cherif, Hamid Saida; Eswayah, Asma; Abdennour, Mohamed Amine; Oliveira, Ivo Vaz
    As a part of the valorisation of medicinal plants of the Algerian flora, we have undertaken a study of plant extract (Juglans regia L.) growing in the Blida region, Algeria. The main objective of this work is to evaluate the in vivo pharmacological effects, namely the anti-inflammatory, antispasmodic and healing effects of common walnuts’s leaves aqueous extracts and ointment. The Carrageenan-induced paw edema test is used for screening of anti-inflammatory activity on 4 batches of mice (control batch, treated batch with Diclofenac®, treated batches with the aqueous extract at 5 and 10%), the analgesic effect was conducted using Acetic acid-induced endogenous spasm test, and the healing effect was conducted on rabbits. The anti-inflammatory test performed on laboratory mice, revealed a remarkable anti-inflammatory effect of the aqueous extract comparing with chosen standard pharmaceutical molecule (Diclofenac®), with a reduction percentage of 25 and 31.72 % for the aqueous extract and Diclofenac® respectively. The results for the analgesic effect was revealed to be significant comparing with those obtained with the reference product Spasfon. Finally, the healing effect was performed on rabbits, revealing high healing power comparing with those obtained with pharmaceutical healing paste ‘MADICASSOL” used as a reference. The ointment prepared using walnut leaves has shown more effectiveness with delay of healing less than a week compared to the ointment of reference which took almost 10 days for total healing effect. The obtained results confirm some information obtained during the ethnobotanical survey in a previous study; and that Juglans regia L. is endowed with an anti-inflammatory, antispasmodic and healing properties
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    Exploring the antifungal properties of tannin extracts from Olea europaea var. rougette leaves against Aspergillus spp.
    (Springer, 2024) Bennacer, Amel; Sahir-Halouane, Fatma; Aitslimane-Aitkaki, Sabrina; Tihar-Benzina, Farida; Oukali, Zahia; Oliveira, Ivo Vaz; Rahmouni, Naima; Aissaoui, Mohammed; Mohankumar, Ramar
    Due to significant health risks associated with chemical antifungal agents in food storage, safe and natural sources are now one of the most frequently demanded ingredient for food preservation. Byproducts from olive trees provide an affordable and sustainable source of bioactive chemicals. This study will examine the mycelial growth and germination-inhibitory properties of Olea europaea var. rougette leaves and characterize their phytochemical makeup.The phytochemical screening revealed the presence of polyphenolics compounds mainly tanins.The chemical composition was analyzed by gas chromatography mass spectrometry (GCMS). Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, potassium iodide and vanilline methods, respectively. The mycelial growth and germination inhibition properties were examined on soft wheat based solid and liquid media. Tannic extract (ethyl acetate extract) revealed highest amounts in polyphenols, hydrolysable tanins and condensed. GC–MS profile of tannic extract showed the presence of 26 bioactive molecules known for their antifungal potential, mainly: Guaiacol (0.12%), 2-methoxy-4-vinylphenol (1.52%), Syringol (0.18%), p-tyrosol (4.70%), homovanillyl alcohol (1.32%), 4-propylresorcinol (1.07%), β-camphor (0.17%), β-turmerone (1.57%) and 4-phenyl-quinolin-2-ol (6.58%). The tannic extract (ethyl acetate fraction) showed significant mycelial growth and germination inhibition activities in contrast to hydro-acetonic and dichloromethanic extracts. Furthermore, myelial growth inhibition showed more effectiveness in comparison to germination inhibition technique.The results suggested that byproducts of Olea europaea var. rougette leaves are a substantial natural source of bioactive compounds, especially tannins with potent antifungal characteristics in vitro which make it interesting to investigate its effectiveness in vivo.
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    Structural characterization of phytochemical content, antibacterial, and antifungal activities of Juglans regia L. leaves cultivated in Algeria
    (Elsevier, 2022) Bennacer, Amel; Sahir-Halouane, Fatma; Aitslimane-Aitkaki, Sabrina; Oukali, Zahia; Oliveira, Ivo Vaz; Rahmouni, Naima; Aissaoui, Mohammed
    Many microorganisms cause diseases to plants, animals and humans. Recently, natural compounds extracted from different medicinal and officinal plants have had a promising antimicrobial potential. In this present study, the antifungal and antibacterial activities of Juglans regia leaves cultivated in Algeria have been evaluated. The chemical composition was analyzed by GCMS spectroscopy. Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, Potassium iodide and vanilline methods respectively. The antibacterial activity was tested against five bacterial strains and the antifungal activity was tested against seven fungal strains. The GC-MS profile of ethyl acetate fraction (tannic extract) revealed the presence of many interesting biomolecules we note: Phenol (0.19%), gaiucol (0.16%), catechol (0.65%), 2-Methoxy-4-vinylphenol (1.77%), eugenol (0.78%), (E)-coniferol (0.66%), 1, 5-naphthalenediol (2.01%) and 5-hydroxy-1, 4-naphthalenedione or juglone (0.18%). The tannic extract (ethyl acetate fraction) presented a high antifungal activity on Aspergillus terreus, Aspergillus ochraceus and Aspergillus Brasilliensis ATCC 16404 with percentage of growth inhibition of 77,033 ± 3,395, 73,327 ± 3,851 and 71,850 ± 0,641 respectively for the highest concentration. It also showed an interesting antibacterial activity against Klebseilla pneumoniae ATCC 70063 with minimal inhibitory concentration of 3.125 μg/ml comparing to the hydro-acetonic extract(MIC value). Our finding provides for the first time the chemical profiling of this species grown in Algeria and also an efficient and targeted natural approach to the discovery of classes of antibacterial and antifungal compounds for various applications