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Author: search.filters.author.Aliouche, DjamelSubject: search.filters.subject.BSA

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    Synthesis, characterization and swelling behavior of pH-sensitive polyvinylalcohol grafted poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) hydrogels for protein delivery
    (Springer, 2023) Hocine, Salima; Ghemati, Djamila; Aliouche, Djamel
    The aim of the current study was to fabricate a pH-sensitive hydrogels for controlled release of Bovine serum albumin (BSA) protein. In this research work, polyvinylalcohol grafted poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) (PVOH-g-poly(AA-co-AMPS)) hydrogels with varying AA/AMPS molar ratio were synthesized through free radical graft copolymerization of partially neutralized acrylic monomers and characterized by Fourier transform infrared spectroscopy, Elemental analysis, X-ray diffraction, and Scanning electron microscopy, thermogravimetric analysis. Then, mechanical proprieties of hydrogels were investigated, and drug release behavior with Bovine serum albumin (BSA) as model drug was studied in vitro. The effect of AA/AMPS molar ratio, pH media on the swelling property of hydrogels was optimized. Results indicated that the strong interaction in the hydrogels resulted in the formation of a more stable copolymer. The high values of the grafting percentage which exceeds 800% confirmed the high performance of the grafting and the successful synthesis. Grafting AMPS onto PVOH/AA improved both thermal stability and mechanical properties of hydrogel. The hydrogel is simultaneous sensitive to pH and ionic strength; the swelling ratio maximizes at pH 6.8 medium and shrinks at pH 1.2, and decreases as the ionic strength increases; the swelling dynamic mechanism was explained well by Fickian diffusion and Schott’s pseudo-second-order models. Different kinetic models were applied, and it has been observed that release profile of BSA best followed the Hixson–Crowell for the release of drug in all release media. However, Zero order and Korsmeyer–peppas models only for pH 1.2 and pH 7.4. The swelling of the hydrogels and release of BSA from the drug loaded hydrogels occurred through non-Fickian diffusion mechanism. It is believed that this hydrogel could be potentially used for localized drug delivery
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    An enhanced immobilization of BSA biomolecule on anionic hydrogels: Swelling and adsorption modeling
    (Springer International Publishing, 2017) Angar, Nour Elhouda; Aliouche, Djamel
    The three-dimensional structure of hydrogels plays a leading role in several areas of applications. The hydrogels are more and more used as systems of immobilized and controlled release of biomolecules in biotechnology and bio-pharmacy industries. To improve protein adsorption capacity in poly(acrylamide) hydrogels, maleic acid co-monomer was included into the reaction mixture during hydrogel synthesis. So, hydrogels of poly(acrylamide) and its copolymers with diprotic maleic acid were prepared by copolymerization and chemical crosslinking with N,N′-methylene bis-acrylamide. Swelling behavior in distilled water, in physiological saline and in bovine serum albumin (BSA) solutions was studied. Influence of initial BSA concentration on hydrogel swelling and BSA adsorption was investigated. The high amount of maleic acid present in the hydrogels has a significant effect on the swelling behavior and BSA adsorption. Results showed that the pH sensitivity of hydrogels resulted in the high amount of adsorbed BSA. The adsorption isotherms were described by Langmuir and Freundlich models. The thermodynamic parameter (ΔG ads 0) was determined for all obtained hydrogels. We demonstrated the favorable character and reversibility of the BSA adsorption process