Publications Scientifiques

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Author: search.filters.author.Benseghir, H.

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    Molecular Surveillance of Yersinia pestis from Stray Dogs and Cats and their Fleas in Algiers
    (Razi Vaccine and Serum Research Institute, 2024) Zaidi, Sara; Bessas, Amina; Hezil, Djamila; Benseghir, H.; Bitam, Idir
    In recent years, plague has re-emerged in several countries around the world and remains endemic in some regions. In a natural environment, and in contact with rodents and their fleas, stray carnivores are most at risk of catching the disease and maintaining the spread of the bacillus. The objective of this study is to demonstrate the presence or absence of Yersinia pestis in stray dogs and cats in the Algiers region by molecular methods and thus contribute their role in epidemiology of this disease. Molecular research of Yersinia pestis has also been conducted on fleas from these carnivores. Preliminary identification of ectoparasites to genus and species level was performed. Real-time polymerase chain reaction targeting Yersinia pestis pla gene was used to survey the plague agent in fleas and carnivores captured as stray animals in Algiers (Algeria). Positive qPCR results were tested by PCR sequencing using glpD gene. Among 327 fleas captured from 107 dogs and 365 fleas from 140 cats, prevalence of Ctenocephalides felis was higher in cats (86,96%), whereas that of Ctenocephalides canis and Xenopsylla cheopis was higher in dogs (90,57% and 92,63% respectively). While internal and external PCR positive controls were positive, none of the 107 dogs spleen and 140 cat spleens and none of the 256 analyzed fleas were positive for Y. pestis. These results suggest that stray cats and dogs are unlikely sources for plague in Algeria contrary to that has been reported in other plague-endemic countries. This observation illustrates that plague epidemiological chain varies from one region to another
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    Chemical composition and antibacterial activity of rosmarinus officinalis essential oil from algeria
    (2021) Hezil, Djamila; Benseghir, H.; Benamrouche, S.; Zaidi, S.; Zineddine, R.; Bessas, A.; Msela, A.
    The aim of this study was to study the chemical composition and the antibacterial activity of the essential oil (EO) of Rosmarinus officinalis from Blida region, against bacterial strains: Escherichia coli, Staphylococcus epidermis and Pseudomanase fragi. The chemical composition of the EO obtained by hydrodistillation was determined using gas chromatography coupled with mass spectrometry (GC-MS). The antibacterial activity of the EO was studied by the disk diffusion method and the agar dilution method in order to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (CMB). The results obtained from this work show that the yield of rosemary EO was 1.6% respectively. The main compounds present in the essential oil of R. officinalis are 2-Bornanone (19.47%), Eucalyptol (18.58%), α-Pinene (9.14%) and Camphene (5.68%). The MIC and CMB values expressed by the essential oil of R. officinalis were identical as for P.fragi and S. epidermidis (MIC is equal to 0.37% and CMB> 3%) and for E .coli were 0.75% (MIC equals CMB). The results of this work confirmed the activity of the EOs of R. officinalis on the pathogenic bacterial strains tested and to recommend its exploitation for use as an alternative to industrial antibacterial molecules.
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    Serological study of salmonella Dublin carriage in cows in khenchela, Algeria
    (2021) Hezil, Djamila; Benseghir, H.; Zaidi, S.; Zineddine, R.; Zaatout, N.; Msela, A.; Ghalmi, F.
    The objective of this study is to estimate the seroprevalence of S. Dublin infection in healthy cows in the Khenchela region and to identify potential risk factors that may be associated with the presence of S. Dublin antibodies. 194 cows sera from 35 farms were tested using an enzyme-linked indirect immunosorbent assay (ELISA) and the results showed a prevalence of 9.23% (18/194, 95% CI 5.17-13.29). The logistic regression model indicates that none of the factors tested were found to be significantly associated with S. Dublin seropositivity. We concluded that S. Dublin circulates in cattle farms in the Khenchela region of Algeria. In addition, we recommend the implementation of hygiene practices and biosecurity measures on farms to reduce the spread of infection and the use of vaccination in animals and people at risk.