Publications Scientifiques

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    Anticancer and antiproliferative activities of Algerian Origanum majorana L.’s essential oil on PC-3 and SKBR3 cells
    (Taylor and francis, 2024) Hafid, Nourehouda; Bouchenak, Ouahiba; Serttas, Riza; Bouhenna, Mustapha Mounir; Khiari, Ouiza; Oussaid, Sounia; Suat, Erdogan
    Cancer is a prominent cause of death globally, with breast cancer and prostate cancer being among the most devastating types. Therefore, the available anticancer treatments have some drawbacks, like higher toxicity and limited bioavailability. Thus, this study aimed to investigate for the first time the anticancer activity of Algerian Origanum majorana L.’s essential oil (OMEO). This research assessed the chemical profile of Algerian OMEO by gas chromatography coupled with mass spectrometry (GC-MS). The analysis revealed 29 compounds, which represent 98.08% of total volatile oil. The major compounds identified in OMEO were terpinen-4-ol (21.37%), γ-terpinene (15.78%), α-terpinene (10.43%), and trans-sabinene hydrate (9.27%). Additionally, MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide) was also used to test the cytotoxicity on prostate cancer (PC-3), breast cancer (SKBR3), and normal retinal pigment epithelium (ARPE-19) cell lines. The results showed a selective cytotoxicity effect by decreasing cell viability of PC-3 cancer cells with half inhibitory concentration (IC50) of 608.57 μg/mL and 672.5 μg/mL after 48h and 72h, respectively. Regarding SKBR3 cancer cells, the IC50 was 991.5 μg/mL. OMEO exhibited no significant cytotoxicity against normal (ARPE-19) cells. Furthermore, we conducted a cell apoptosis assay using Hochest 33342 dye to explore the potential mechanism pathway of OMEO. The findings verified that OMEO could trigger apoptosis in PC-3 and SBKR3 cancer cells. The ability of OMEO to inhibit cell migration assessed via wound healing assay revealed a significant decrease in cell migration. Our results imply that OMEO decreases cell viability by inducing cell apoptosis. Moreover, the oil suppresses cell migration in prostate cancer and breast cancer cells.
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    Students’ perceptions regarding the abrupt transition to online learning during the COVID-19 Pandemic: Case of Faculty of Chemistry and Hydrocarbons at the University of Boumerdes—Algeria
    (American Chemical Society, 2020) Blizak, Meriem Djanette; Blizak, Salah; Bouchenak, Ouahiba; Yahiaoui, Karima
    In recent months, universities around the world have been forced to cancel courses and close their doors due to the growing coronavirus epidemic. Following the government’s precautions, Algerian students were bound to quarantine regulations for their safety. Thus, they unexpectedly switched to online learning instead of face-to-face learning. Our objective of this exploratory study is to investigate the perceptions of Algerian university students regarding the abrupt transition to online learning amid the COVID-19 pandemic. To achieve this goal, an online survey with closed and open-ended questions was conducted among 380 students from the faculty of chemistry and hydrocarbons (FCH) at the University of Boumerdes—Algeria. The results showed that students have a negative perception of online learning. They are reluctant about this new digital pedagogy and prefer the traditional way of teaching to online teaching during the coronavirus pandemic
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    MALDI-TOF MS detection of endophytic bacteria associated with great nettle (Urtica dioica L.), grown in Algeria
    (2018) Toubal, Souheyla; Bouchenak, Ouahiba; Elhaddad, Djillali; Yahiaoui, Karima; Boumaza, Sarah
    Any plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. The purpose of this work is to isolate and to identify the different bacteria from the internal tissues of Urtica dioica L. and to study their diversity. This last is based on the different parts of the plant (stems, leaves and roots) and the harvest regions (Dellys and Tlamcen). The identification of bacteria is done by biochemical tests and confirmed by MALDI-TOF MS. Seven genus and eleven species were isolated from the Great Nettle. They belong to the genera Bacillus, Escherichia, Pantoea, Enterobacter, Staphylococcus, Enterococcus and Paenibacillus. The majority of these bacteria were isolated from Tlemcen which makes this region the richest in endophytic bacteria compared to that harvested from Dellys. The results show also that the leaves are the most diversified in endophytic bacteria. Bacillus pumilus-ME is the common species of the three parts of the plant harvested in both regions. From this work, it emerges that the Great Nettle can be settled by various endophytic bacteria which are differently distributed within the same plant harvested in different regions