Publications Scientifiques

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    Contribution to the Study of Biotechnological Control Techniques Applied Against Biocorrosion Oil Installations
    (Revista de Chimie SRL, 2021) Khoukhi, Faiza; Nacer-Eddine, Djelali; Kebouche, Salima; Gana, Mohamed Lamine
    The biological activity of oil extracted from green algae has long been known, but evaluation of bimolecular activities contained in this oil on an industrial scale, especially in the oil industry, was the objective of our research project. The first stapes of this study is to extract the essential oil from green algae marine Ulva Lactuca in our region, obtained by extraction/purification method based on hydro distillation and methanol extraction, the work performed at the laboratories of Sonatrach (SH/DTD/AUI/CEM). The second stapes is to evaluate the potential of these algae extracts on microbiologically influenced corrosion. After chemical-physic characterization of two bio naturals products A and B, we test their effectiveness as a bactericide on bacterial corrosion of carbon steel in water contained sulfate reducing bacteria by electrochemical analysis techniques (Open-circuit potential/OCP and Electrochemical Impedance Spectroscopy/EIS).
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    Efficiency Evaluation of Anti-corrosion Treatment of Carbon Steel by Extracts of Red Algae Collected from Mediterranean Coast
    (Revista de Chimie SRL, 2021) Khoukhi, Faiza; Kebbouche-Gana, Salima; Djelali, Nacer Eddine; Gana, Mohamed Lamine
    The paper presents the assessment of inhibition effectiveness of biomolecules extracted from red seaweed against biocorrosion in the petroleum industry. The first objective of this study was to obtain extracts (A, B and C, prepared respectively from red algae species: Corallina ellongata, Gymnogongrus crenulatus and Pterocladia capillacea) by ethanol extraction method. The infrared spectra of the three extracts confirmed the presence of amine derivatives molecules known by their anti-corrosion inhibiting powers. The second objective was based on the identification of physico-chemical characteristics of the extracts and thus revealing their inhibitory and / or bactericidal power in bacterial corrosion of carbon steel in injection water contaminated with sulfato-reducing bacteria. Biological test of all extracts gave a concentration upto 10 germs/mL in contaminated water by sulfato-reducing bacteria during 28 days of incubation at 37°C. Evolution in time of the open-circuit potential showed a longer incubation time for electrolyte with extracts, whereas the stabilization time was shorter. Current corrosion density, polarization resistance, charge transfer resistance and double layer capacity were determined by using linear polarizarion resistance technique and electrochemical impedance spectroscopy. The corrosion protection efficiency of extract obtained from Gymnogongrus crenulatus (extract B) reached a maximum protective capacity of 99.69% at 5 ppm in the injection water.
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    Diesel Biodegradation Capacities and Biosurfactant Production in Saline-Alkaline Conditions by Delftia sp NL1, Isolated from an Algerian Oilfield
    (Taylor and Francis Inc., 2020) Lenchi, Nesrine; Kebbouche-Gana, Salima; Servais, Pierre; Gana, Mohamed Lamine; Llirós, Marc
    In this study, a diesel oil-degrading bacterium was isolated from an oilfield water injection (water-bearing formations, 1,205 m depth) in Algeria. The bacterial strain, designated NL1, was cultivated on diesel oil as sole carbon and energy sources. Molecular analyses of the 16S rRNA gene sequence (KY397882) placed NL1 strain closely related to distinct cultivated species of the Delftia genus. Optimal diesel oil biodegradation by Delftia sp NL1 strain occurred at pH 11, 40 °C, 2 M NaCl and initial hydrocarbon concentration of 5% (v/v) as sole carbon source. GC-MS analyses evidenced that strain Delftia sp NL1 was able to degrade more than 66.76% of diesel oil within only 7 days. On the other hand, and in the same conditions, biosurfactant production by Delftia sp NL1 was also evaluated evidencing high emulsifying capacity (E24 = 81%), ability to lower the surface tension of growing media (with the value of 25.7 mN m− 1), and production of glycolipids (8.7 g L−1) as biosurfactants. This research presents indigenous strain Delftia sp NL1 for diesel degradation and synthesis of biosurfactant in extreme conditions. In this sense, strain NL1 is a good candidate for possible in situ oil recovery and in wastewater treatment in refineries and oil terminals in petroleum industry
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    The Application of Enzymological Techniques for Determination of the Inhibitor Activity of Bio Enzym Extracted from Seawater Bacteria P s eudomonas aerogenosa to Fight Biocorrosion of Carbon steel
    (Central de Documentare Al Industriei Chimice Ri, 2017) Khoukhi, Faiza; Kebouche, Salima; Djelali, Nacer-Eddine; Gana, Mohamed Lamine
    The paper presents a studies for the development of new corrosion inhibitors by biotechnological way applicable to many areas of petroleum industry including, characterization of protective films on carbon steel and biocorrosion inhibition. Our research is oriented towards the isolation of new strains of bacteria from the sea waters of the Mediterranean region that have power (inhibitory and/or bactericidal that blocks the growth of sulfate-reducing bacteria responsible for microbiologically influenced by production of Pyocyanin (1 hydroxyphenazin) or 5-methylphenazin-1-one. Our first objective was to isolate the population of Pseudomonas Aerugenosa on a specific medium, characterization gram, then fermentation in a nutrient broth. The second objective of this study is evaluation of corrosion rate by weight loss method with injecting different dose of the crude enzyme extract CEE containing the methylphenazine (Pyocyanin). An efficacy test was performed on test kit vials containing the specific culture medium with the SRB contaminated with industrial injection water from SONAHESS region. The total disappearance of the SRB is 40% of CEE by blocking their metabolism in the growth phase confirms the efficiency of treatment
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    Production of single cell protein from organic whey using kluyveromyces fragilis and candida kefyr strains
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Bouanane-Darenfed, N.A.
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    Crude date syrup as fermentation medium for biosurfactant production by Natrialba sp. Strain E21
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Bouanane-Darenfed, N.A.
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    Biotechnology-2014 biodegradation performance of phenol by free and alginate entrapped cells of Haloarcula strain D21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria)
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Khemili, Souad
    Immobilization is a general term that describes many different forms of cell attachment or entrapment. These different forms include encapsulation of cells in a polymer-gel and entrapment in a matrix. The Advantages of these techniques are to reduced possibility of inoculum contamination during storage, transport and application. So, beads are non toxic, biodegradable and non-polluting and can be produced in large quantities, stored for extended periods. Moreover, phenols and phenolic compounds are widely distributed as environmental pollutants due to their common presence in the effluents of many industrial processes, including oil refineries, ceramic plants, coal conversion process, phenolic resins, pharmaceutical and food Industries. The aim of this study was to compare the biodegradation performance of phenol by using free and encapsulated cells of Haloarcula strain D21 isolated from crude oil contaminated saline water collected at Ain Salah in Algeria. Batch experiments were carried out in order to obtain the maximum phenol degradation rates by analyzing the influence of the immobilization in calcium-alginate gel beads on biodegradation performance
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    Production of biosurfactant on crude date syrup under saline conditions by entrapped cells of Natrialba sp. strain E21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria)
    (Springer, 2013) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Ferrioune, Imen; Khemili, Souad; Lenchi, Nesrine; Akmoussi-Toumi, Siham; Bouanane-Darenfed, Nabila Amel; Djelali, Nacer-Eddine
    A bacterial strain E21 was isolated from a sample of water collected in the salt lake located close to Ain Salah, Algeria. The analysis of 16S rRNA gene sequence had indicated that the strain had 93 % sequence similarity with the genus Natrialba sp. strain E21 (Gen- Bank, FR750525.1) and was considered extremely halo- philic. Production of biosurfactant by the strain E21 with free and entrapped cells was investigated using soluble starch in the saline conditions. Biosurfactant synthesis was followed by measuring the surface tension and emulsifying index 9 days under optimal conditions (40 C, pH 7). Some diffusional limitations in alginate and agar beads affected the kinetics of biosurfactant production when compared to that obtained with free cells culture. The minimum values of surface tension were 27 and 30 mN m - 1 achieved after 9 days with free and immobilized cells, respectively, while the corresponding maximum E24 values were 65.3 and 62.3 %, respectively. The re-use of bacterial cells along with the limited cell losses provided by the immobilized system might lead to significant reduction of the biosur- factant production cost