Publications Scientifiques
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Item Simultaneous removal of crude oil and heavy metals by highly adapted bacterial strain Cutibacterium sp. NL2 isolated from Algerian oilfield(Springer Science and Business Media Deutschland GmbH, 2024) Lenchi, Nesrine; Ahmedi, Wissam Nour El Houda; Llirós, MarcInvestigating the ability of bacteria to simultaneously enhance hydrocarbon removal and reduce heavy metals’ toxicity is necessary to design more effective bioremediation strategies. A bacterium (NL2 strain) isolated from an Algerian oilfield was cultivated on crude oil as sole carbon and energy sources. Molecular analyses of the 16S rRNA gene sequence placed the strain within the Cutibacterium genera. This isolate was able to tolerate up to 60% of crude oil as sole carbon source. Chemical analyses (GC-MS) evidenced that strain NL2 was able to degrade 92.22% of crude oil (at optimal growing conditions: pH 10, 44 °C, 50 g L−1 NaCl, and 20% of crude oil (v/v) as sole carbon source) in only 7 days. NL2 isolate was also able to produce biosurfactants with reduction of surface tension of growing media (29.4 mN m−1). On the other hand, NL2 strain was able to tolerate high lead (Pb) and copper (Cu) concentrations (up to 60 mM). In fact, NL2 cultivated in the presence of 20% of crude oil, and 0.48 mM of Pb was able to reduce Pb concentration by a 41.36%. In turn, when cultivated on high Pb concentration (15 mM), the strain was able to remove 35.19% of it and 86.25% of crude oil, both in a time frame of 7 days. Our findings suggest that Cutibacterium strain NL2 is able to efficiently use and remove a wide range of crude oil substrates in presence of high Pb concentration. Accordingly, NL2 strain is of extreme interest from a biotechnological standpoint.Item Microbacterium algeriense sp. nov., a novel actinobacterium isolated from Algerian oil production waters(Microbiology Society, 2020) Lenchi, Nesrine; Anzil, Adriana; Servais, Pierre; Kebbouche-Gana, Salima; Gana, Mohamed LamineA non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i.e., strain G1T) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3-3.5 % (w/v) NaCl, at 20-50 °C and at pH 6.0-9.0. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that strain G1T belonged to the genus Microbacterium. Strain G1 T was closely related to Microbacterium oxydans (DSM 20578T) and Microbacterium maritypicum (DSM 12512T) with 99.8 % sequence similarity and to Microbacterium saperdae (DSM 20169T) with 99.6 % sequence similarity. Strain G1 T contained MK9, MK10, MK11, MK12 and MK13 as respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol and glycolipid as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The estimated DNA G+C content was 69.57 mol% based on its draft genome sequence. Genome annotation of strain G1T predicted the presence of 3511 genes, of which 3483 were protein-coding and 47 were tRNA genes. The DNA-DNA hybridization (DDH) and average nucleotide identity (ANI) values between strain G1T and M. oxydans (DSM 20578T) and M. maritypicum (DSM 12512T) were in both cases far below the respective species boundary thresholds (27.5 and 28.0 % for DDH; and 84.40 and 84.82% for ANI, respectively). Based on the data presented above, strain G1T was considered to represent a novel species for which the name Microbacterium algeriense is proposed with the type strain G1T (=DSM 109018T=LMG 31276T)Item Comparison of microbial community composition in injection and formation water from Algerian oilfields(2012) Lenchi, Nesrine; Inceoğlu, Özgül; Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Llirós, Marc; Servais, Pierre; Garcia-Armisen, TamaraItem Production de biosurfactants par Haloarcula sp. souche D21 en conditions salines à partir de milieux de culture à base de lactosérum et des eaux de trempage de maïs (corn steep liquor)(2012) Kebbouche-Gana, Salima; Khemili, Souad; Lenchi, Nesrine; Akmoussi-Toumi, Siham; Ferioune, I.Item Phenotypic characterization, 16s RNA identification of cultivable on-obligate halophilic bacterial communities from a Tinsilt sebkha, wilaya of Oum El Bouaghi (Algeria) and screening for extracellulars hydrolases and biosurfactants(2013) Kebbouche-Gana, Salima; Lenchi, Nesrine; Gana, Mohamed Lamine; Khemili, SouadItem Production of an extracellular lipase from a halophilic isolate, Idiomarina sp.(2013) Akmoussi-Toumi, Siham; Kebbouche-Gana, Salima; Lenchi, NesrineItem Microbial community composition in production and injection waters from Algerian oilfields(2013) Lenchi, Nesrine; Inceoğlu, Özgül; Kebbouche-Gana, Salima; Garcia-Armisen, TamaraItem Homology modelling and bioinformatics analysis of haloarchaeal α-amylases : an overview of proteins haloadaptation and stability(2013) Khemili, Souad; Zorgani, Mohamed Amine; Kebbouche-Gana, Salima; Lenchi, Nesrine