Publications Scientifiques
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Item Conservation of Deglet Nour variety dates in syrup form and study of their quality(Universidad de Costa Rica, 2025) Lagha-Benamrouche, Samira; Hezil, DjamilaDeglet Nour date syrup is gaining popularity due to its unique flavor and potential health benefits. Assessing its quality is crucial for both consumers and producers, as it directly influences its market value and health reputation. Objective. To evaluate the nutritional value, sensory quality, and antioxidant potential of Deglet Nour dates in syrup form. Materials and methods. The biological material was collected in Biskra, Algeria, in December 2019, during the maturation period of the plant material. To determine the various components of biological substances, quantitative analysis through color reactions was employed to measure total sugars, reducing sugars, non-reducing sugars, proteins, polyphenols, and flavonoids. The amounts of lipids and ash were recorded, and an atomic absorption spectrophotometer was applied to assess the variety of minerals. Testing for antioxidant activity was conducted through antiradical experiments. Furthermore, sensory evaluation included 45 individuals who assessed this syrup against others, concentrating on clarity, sweetness, and general flavor preference. Results. The chemical composition analysis revealed an average total sugar content (52 %), a moderate amount of protein (1.6 %), and low-fat content (0.09 %). The energy content was 215.6 kcal/100 g. The mineral analysis showed that the syrup is rich in essential minerals, particularly calcium, sodium, and magnesium. Flavonoids were identified as the predominant antioxidants, with anti-free radical activity e stimated at 27.62 %. The sensory evaluation compared Deglet Nour syrup to Ghers and Degla-Beida varieties. The classification test showed that Deglet Nour syrup was perceived as the clearest. While it did not differ significantly in sweetness from Degla-Beida, it was preferred by tasters for its more pleasant taste. Conclusions. Dates of the Deglet Nour variety, which are highly sensitive to deterioration, yield after processing a natural syrup with the desired nutritional, therapeutic, and sensory properties.Item Statistical optimization of microwave-assisted extraction of phytochemicals from Retama raetam (white weeping broom) twigs and their biological properties(AfAc Publisher, 2024) Zaoui, Oussama; Oughlissi-Dehak, Karima; Bouziane, Mebarkaackground: Several phytochemicals derived from the genus Retama reported to possess diverse biological activities, including antioxidant, anti-inflammatory, and antibacterial properties. Aims: The aim of this study was to optimize microwave-assisted extraction (MAE) of polyphenols from Retamaraetam twigs using response surface methodology. Methods: A Box-Behnken design was utilized for determining the effect of MAE factors on total polyphenol content (TPC), including ethanol concentration (50 – 70%), irradiation time (4 – 6 min), power (400 – 600 W), and solvent-to-sample ratio (15 – 25 mL/g). The optimal extract (OE) was further analyzed for total flavonoid content (TFC), total tannin content (TTC), and antioxidant activity (DPPH• scavenging and FRAP) andin vitro anti-inflammatory activity assessment of the OE was evaluated using two complementary assays (albumin denaturation and membrane stabilization). Results: The following conditions: ethanol concentration of 64.73%, irradiation time of 5.57 min, power of 569.16 W, and solvent-to -sample ratio of 22.91 mL/g, resulted in the highest TPC (181.48 ± 1. 59 mg GAE/g DR). The effectiveness and statistical validity of the derived quadratic model indicated no significant discrepancies between experimental and predicted results, demonstrating its high degree of accuracy. The obtained OE demonstrated a TFC of 31.25 ± 1.5 mg EC/g DR and a TTC of 15.17 ± 1.56 mg EC/g DR. The OE showed a significant capacity to scavenge DPPH• and an appreciable ferric-reducing power, where the IC50 and EC50 values were respectively 0.44 ± 0.08 and 0.61 ± 0.03 mg/mL. At a concentration of 1.5 mg/mL, the OE displayed moderate anti-inflammatory activity by red blood cell membrane stabilization (72.72 ± 0.73%) and reduction of heat-induced albumin denaturation (50.89 ± 0.66%). Conclusion: The MAE of TPC from Retama raetam twigs was primarily influenced by EtOH concentration, irradiation time, and power. The OE exhibited moderate antioxidant and anti-inflammatory properties, suggesting its potential as a source of phytopharmaceuticals.Item Evaluation of gastroprotective effect of jujube honey in ethanol-induced stomach ulcer in mice(NIS PR, 2023) Benmouloud, Abdelouafi; Zatra, Yamina; Belkadi, Asma; Halli, Latifa; Kacim, Lilia; Souame, Samir; Aknoun-Sail, Naouel; Khaldoun, MouniraHoney has been widely documented to have therapeutic benefits, especially on the healing of wounds, digestive disorders, and as an antimicrobial agent. It’s known that stomach ulcers are one of the most prevalent diseases that affect humans with no effective treatment. The objective of this study was to investigate the gastroprotective effects of jujube honey against ethanol-induced gastric ulcer in mice and to explore possible mechanism of its action on the gastric mucosa. Stomach’s tissues were examined for ulcerative lesions and white blood cell count (WBC), plasma C-reactive protein (CRP) and nitric oxide (NO) for clinical inflammation evaluation. The antioxidant enzyme activities were investigated using the measurement of superoxide dismutase (SOD) and catalase. Treatment with jujube honey reduced the ulcer index and preserved gastric tissue. It also decreased the inflammatory response by reducing WBC and plasma levels of CRP and NO in ulcerative group. In addition, jujube honey-treated group showed increased antioxidant enzyme activities of catalase and SOD. Furthermore, diluted jujube honey is more effective in preserving mucosal gastric integrity. In conclusion, these findings suggest that jujube honey, especially with dilution, may exert a protective effect against ethanol-induced gastric ulcer, through the improvement of antioxidant system and suppression of the inflammatory response via its bioactive compounds.Item Valorization of carob seeds as a functional food(2023) Lagha-Benamrouche, Samira; Boudjema, Khaled; Rezgui, Walid; Djeziri, Mourad; Hezil, DjamilaOur study aims to promote carob seeds as a functional food. For this; the nutritional value, the compounds with beneficial physiological effects and functional properties (fibers and gums), as well as the antioxidant potential were determined. Analysis of the chemical composition of carob seeds reveals their high protein, ash and fat content. However, the total sugars content was estimated to be moderate. The quantification of the compounds with a beneficial physiological effect shows that the seeds are rich in crude dietary fibers (8.39%). Regarding the gums, the yields are evaluated at 39.44% for the crude gums and at 4.026% for the purified gums. The phytochemical assays reveal a richness of the seeds in total polyphenols, in total flavonoids with a moderate content of flavonols and hydrolyzable tannins. The antioxidant potential was studied using two methods: reduction of the free radical 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and the iron reduction method. According to the results, the carob seeds have a discreet antioxidant potential compared to the standards tested (gallic acid and quercetin).Item Oxidative Stability improvement of diesel fuel during the storage(University M'hamed Bougara - Boumerdes, 2020) Mesbah, Y.; Rahawi, K.; Cheikh, S.; Benmabrouka, Hafsa; Hamada, B.In this work a biobasedmaterial which is an antioxidant additive for diesel fuel during the storage was synthesized. Pomace olive oil extracted by maceration with hexane as raw material was chosen in the experimental process. Further, olive pomace wastes were fetched from a traditional oil mil. The involved processes were epoxidation by oleic acid of the pomace olive oil, opening of the oxirane ring, and esterification; respectively. The structures of the products were characterized and identified by both FTIR spectroscopy and by physic-chemical analysis methods. The effectiveness of our biobased antioxidant additive was tested by realizing an accelerated oxidation test (AOT) on the Algerian diesel of first by catalytic cracking without additive (blank) and further by testing diesel with different additive concentrations such as 2000, 3000, 4000 and 5000 ppm, respectively. The oxidative degradation of diesel with or without antioxidant was followed by sediment contents according to ASTM D2274. Diesel stability with antioxidant additive compared with the blank confirms the inhibition of the oxidation phenomena. Further the best antioxidant additive efficiency was recorded at the optimal concentration of 4000ppmItem Antioxidant and wound healing potential of saponins extracted from the leaves of Algerian urtica dioica L.(2017) Laoufi, Razika; Affif Chaouche, Thanina; Chebouti-Meziou, Nadjiba; Benhabyles, Narimen; Dahmani Mohamed, Mahdi; Arab, KarimItem Synthesis of a biobased antioxidant additive for diesel fuel(2014) Hamada, Boudjema; Zourane, Tinhinane; Pemanos Yelda, Bakoz; Chikh, SabrinaIn this study, our objective is to synthesize a biobased antioxidant additive for Diesel fuel during the storage. Our choice was made particularly on the olive oil as raw material because of its oleic acid content which is more to 75%. The processes involved were epoxidation of oleic acid of the olive oil, opening of the oxirane ring, and esterification. The structures of the products were confirmed by FTIR. To test the effectiveness of our biobased antioxidant additive, we carried out Accelerated Oxidation Test – AOT on diesel without additive (blank) and diesel with additive with various concentrations: 2000,4000 and 6000 ppm. The oxidative degradation of diesel with or without antioxidants was investigated by UV-visible spectroscopy was used to monitor the changes using peroxide value. A remarkable stability of diesel with antioxidant additive comparing with the blank confirms the inhibition of the oxidation phoneme and thus of the good efficiency of antioxidant additive from the optimal concentration of 2000ppm