Publications Scientifiques
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Item A novel anti-candidiasis cream formulation based on Melissa officinalis and Lavandula stoechas essential oils synergism(Taylor & Francis, 2024) Rezzoug, Hamida; Bouchenak, Ouahiba; Boudjema, Khaled; Benabdelkader, Tarek; Felix, Tomi; Pierre, Tomi; Djilani, SelmaCandida albicans is the yeast strain that causes candidiasis, which can vary from minor skin and soft tissue infections to potentially fatal infections like candidemia, which can get worse due to drug resistance. Natural substances could be used as antifungal agents’ alternatives, especially against C. albicans. Thereby, the main objective of this research was to define the chemical content and evaluate the bioactivity of Melissa officinalis and Lavandula stoechas essential oils (EOs). Additionally, the synergistic effect of both oils was carried out against C. albicans, in order to formulate EOs combination-based creams. Their chemical compositions were analyzed using the combination of gas chromatography-flame ionization detector (GC-FID) and GC-mass spectrometry (GC-MS). The interaction with both EOs was also evaluated and analyzed by GC-FID and GC-MS, and the development of anti-candidiasis formulations was tested in vitro. Our results showed that the main compounds of M. officinalis EO (MOEO) were geranial (35.7%) and neral (24.1%), whereas, 1.8-cineole (61.9%) was dominant in Lavandula stoechas essential oil (LSEO) and the main compounds of the combination between both EOs were 1,8-cineole and geranial. Moreover, the MOEO exhibited substantial antifungal action against all tested microorganisms with MIC values ranging from 0.125 to 0.0019. This EO also showed synergistic effect when combined with that of L. stoechas. These findings, suggest the good bioactive qualities of the topical creams developed against C. albicans, revealing their potential to be possibly applied as an antifungal agent in industrial pharmaceutics.Item Extraction and valorization of active principles of Saccocalyx satureioides collected in two wilayas of Algeria(ALJEST, 2022) Draoui, Yasmine; Aksas, h.; Amghar, F.The study of Saccocalyx satureioïdes belonging to the Lamiaceae family and collected from two regions of Algeria, namely: Ain chouhada de Djelfa and Zaafrane de M'sila, allowed to characterize the different families of chemical compounds contained in its aerial part, The analysis of Saccocalyx saiureioïdes essential oils of the two regions by gas chromatography coupled with mass spectrometry (GC/MS), allowed to identify the following major constituents (borneol 13. 24%, α-Terpineol 27.42% and thymol 34.87%) for the Zaafrane site and (borneol 11.9%, α-Terpineol 14.9% and thymol 41.09%) for the Ain chouhada site, in Saccocalyx satureioides,.Item Chemical composition and antibacterial activity of rosmarinus officinalis essential oil from algeria(2021) Hezil, Djamila; Benseghir, H.; Benamrouche, S.; Zaidi, S.; Zineddine, R.; Bessas, A.; Msela, A.The aim of this study was to study the chemical composition and the antibacterial activity of the essential oil (EO) of Rosmarinus officinalis from Blida region, against bacterial strains: Escherichia coli, Staphylococcus epidermis and Pseudomanase fragi. The chemical composition of the EO obtained by hydrodistillation was determined using gas chromatography coupled with mass spectrometry (GC-MS). The antibacterial activity of the EO was studied by the disk diffusion method and the agar dilution method in order to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (CMB). The results obtained from this work show that the yield of rosemary EO was 1.6% respectively. The main compounds present in the essential oil of R. officinalis are 2-Bornanone (19.47%), Eucalyptol (18.58%), α-Pinene (9.14%) and Camphene (5.68%). The MIC and CMB values expressed by the essential oil of R. officinalis were identical as for P.fragi and S. epidermidis (MIC is equal to 0.37% and CMB> 3%) and for E .coli were 0.75% (MIC equals CMB). The results of this work confirmed the activity of the EOs of R. officinalis on the pathogenic bacterial strains tested and to recommend its exploitation for use as an alternative to industrial antibacterial molecules.Item Microencapsulation of Thymus fontanesii extracts in pectin/casein : characterization, release behavior and storage stability(Springer, 2021) Mebarki, Noudjoub; Ziane, Hanifa; Fazouane, Fethia; Nabiev, MohamedThe present study aims to enhance the stability of Thymus fontanesii essential oil (TEO) and its main component thymol, and to prolong their release from encapsulation in pectin/casein matrix using complex coacervation and dried by freeze methods. Encapsulation efficiency, structural, physical and thermal properties of the microcapsules were determined. Storage stability of free and microencapsulated thyme extracts was evaluated and the controlled release kinetics of microcapsules in simulated gastrointestinal fluids were studied. Respectively, the successes of TEO and thymol encapsulation were confirmed by FTIR and X-ray diffraction analyses with encapsulation efficiency of 91.85 ± 1.35% and 93.66 ± 1.13%. The particle size ranged from 4.47 to 517.20 μm, presented a good thermal resistance and irregular shapes with rough surface. Prolonged release of the extracts from microcapsules was demonstrated with Higuchi behavior in gastric fluids while for simulated intestinal fluid, the non-Fickian transport was the main release mechanism. The microencapsulation of thyme extracts enhanced its thermal stability and proved that the thyme extracts were effectively well protected in microcapsules after a long time storage. The use of pectin/casein has shown good performance as a biodegradable matrix for protection and storage of thyme extracts, prolonging their storage stability and release. Therefore, these microcapsules can be useful ingredients for both food and pharmaceutical new products, to prolong bioavailability of bioactive compounds and to improve their thermal and storage stabilityItem Evaluation of biological activities of Thyme extracts and their use as a naturalalternative to antibiotics for the reduction of pharmaceutical waste(Technology Publishing Group, 2019) Mebarki, Noudjoub; Nabiev, M.; Ziane, H.; Chader, H.; Fazouane, F.Excessive and improper use of chemical antibiotics is dangerous to human health and animals and causes an unhealthy environment. Hence, medicinal plants are expected to be the future alternative source of new antimicrobial products. The present study aimsto determine the chemical composition of Thymus fontanesiiBoiss. & Reut.essential oil (EO) then to isolate andcharacteriseitsmain constituent,thymol,and to evaluatetheir antibacterial and antioxidant activities. The oil was obtained by hydro-distillation from the aerial parts of T. fontanesii at yield of 3.4±0.2% and 06 main compounds were identified using GC/MS technique representing more than 99% of the oil composition. The major constituents were thymol (77.72%) and carvacrol (13.27%). The antimicrobial activity of our extracts was investigated in comparison with four synthetic antibiotics, using the disc diffusion and broth macro-dilution methods against 13 pathogenic bacteria. The antimicrobial evaluation showed that EO and thymol isolate exhibited high antibacterial activity against all the strains tested compared with the synthetic antibiotics, they were capable of inhibiting the growth of bacterial organisms tested with inhibition zones in the range of 10–58.53 mm. The EOwas the most active with MIC and MBC values of (0.035 mg/mL to 0.565 mg/mL). The antioxidant activity of our extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH)radical scavenging method.The antioxidant activity revealed that the EO and thymol isolate demonstrated a very important anti-radical activity compared to the standards (ascorbic acid and BHT). T. fontanesii essential oil showed the strongest (p < 0.05) radical scavenging effect, with 50% inhibitory concentration (IC50) of 15.85 ± 0.23μg/mL. The results showed that the essential oil of T. fontanesii and thymol, its major compound, are an excellent alternative to synthetic antibiotics. They are of great interest because of their availability, non-toxicity and their friendship with the environment, and therefore can be useful for the food and pharmaceutical industries.Item Preparation, characterization andin vitroantimicrobialactivity evaluation of thyme essential oil formulation totreat skin infections(University of El Oued, 2020) Mebarki, Noudjoub; Nabiev, M.; Ziane, H.; Chader, H.; Fazouane, F.The aim of the present study was to develop and evaluate a topical formulation containingThymus fontanesiiBoiss. & Reutessentialoilthat could serve as an antimicrobial agent forthe treatment of various skin diseases caused by bacteria,yeastanddermatophytes.Theselected formulationexhibited a significant antimicrobial activity against all tested strains,betterconsistency, good homogeneity, no skin irritation and good stabilityforsixmonths at25 ± 2°C.Our results demonstrate thatthe formulationcould beused safely as topicalpreparationto treat skin diseases caused by the tested microorganismsinto the futureItem Optimization of the Yield Extraction of Essential Thyme Oil through Response Surface Method (RSM)(Society of Pharmaceutical Tecnocrats, 2020) Ayati, Fadila; Touzouirt, Saida; Aomari, Lila; Sehaki, ChabhaThe objective of this study is the optimization of the extraction yield of essential Thyme capitatus oil using hydrodistillation Clevenger in order to industrialize the scale and increase the extrapolation possibility. The optimal conditions are determined using central composite designs (CCD). Nine runs were performed by varying the extraction time and the plant material form (i.e. ground and unground). The ratio of the mass/volume was considered constant during the experiments. The optimum yield was obtained for unground form with 1.6078% during one hour of extraction. The results of the extracted oil showed that the relative density values and the refractive index comply with international standardsItem The essential oil of Algerian Ammodaucus leucotrichus Coss. & Dur. and its effect on the cholinesterase and monoamine oxidase activities(Elsevier, 2018) Sadaoui, Nesrine; Bec, Nicole; VéroniqueBarragan, Montero; Kadri, Nabil; Cuisinier, Frederi; Larroque, Christian; Arab, Karim; Khettal, BachraThis study aims at performing a chemical analysis of the essential oil (EO) of Ammodaucus leucotrichus Coss. & Dur. and investigating the anticholinesterase and anti-monoamine oxidase activities. The EO of the A. leucotrichus aerial part was analyzed by Gas Chromatography Mass Spectrometry (GC-MS). The inhibitory potency against Acetylcholinesterase (AChE) and Butyrylcholinesterase (BChE) were evaluated in vitro using modified Ellman's method. Monoamine oxidase activity was measured using the spectrophotometric method described by Weissbach. The chemical analysis of EO showed that the main constituents were perillaldehyde (by about 58.3%) and limonene (by about 23.33%). Further, the essential oil extracted from A. leucotrichus exhibited a promising anti-butyrylcholinesterase activity with an IC50 of about 95.2 μg/mL, where the perillaldehyde and limonene IC50 corresponded to 42.7 μg/mL and 66.7 μg/mL, respectively. The anti-acetylcholinesterase activity was observed only with limonene with IC50 = 51.6 μg/mL. The highest monoamine oxidase inhibitor activity was observed by essential oil followed by perillaldehyde. The oil demonstrated IC50 values for monoamine oxidase-B (MAO-B) of 40.5 μg/mL and 98.9 μg/mL for perillaldehyde. While, IC50 values for monoamine oxidase-A (MAO-A) were 112.5 μg/mL and 159.1 μg/mL for EO and perillaldehyde, respectively. No inhibitory activity against monoamine oxidase has been reported with limonene. A. leucotrichus C. & D. essential oil may represent an alternative source useful as a moderate anticholinesterase agent, particularly against butyrylcholinesterase and monoamine oxidase.Item Assessment of Some Biological Activities of Eucalyptus blakelyi Maiden Using the Essential Oil, Methanolic and Aqueous Extracts(Har Krishan Bhalla and Sons, 2020) Boukhalfoun, Leila; Kirouani, A.; Behidj, N.; Gana, S.Several species of Eucalyptus are used in folk medicine as an antiseptic, anti-inflammatory, and against respiratory infections, such as colds, sinus congestion and influenza. These species have shown potential antioxidant properties in addition to antimicrobial activities. In the present paper, we were interested in Eucalyptus blakelyi Maiden, a species belonging to the Myrtaceae family. Our study can be considered as the first detailed report on the chemical composition, antioxidant and antimicrobial activity of different extracts. The aim of the current study is to evaluate the biological activities of essential oils, methanolic and aqueous extracts of this plant. The present work was realized on leaves collected from National Institute for Forest Research (INRF) of Baïnem (Algiers, Algeria), and the results showed that essential oil’s yield obtained by hydrodistillation was 2.13 %. Actually, 15 compounds were identified by GC-MS profile, and the major compound was 1.8-cineole with 62.74 %. Indeed, the antiradical activity using the DPPH method has shown that methanolic and aqueous extracts had an interesting antioxidant ability with an IC50 of 0.33 and 0.39 mg/ ml respectively. The antimicrobial activity of the essential oil, methanolic and aqueous extracts on eleven microbial strains was evaluated using the aromatogram method. Thus, the methanolic extract proved to be the most active compared to the aqueous extract and the essential oil by inducing a maximum inhibition zones of 19.66 mm for Staphylococcus aureus ATCC29213 and 25.66 mm for Candida albicans. The results suggest potential applications of essential oil in food and extracts in pharmaceutical industryItem Antibacterial activity of cumin (cuminum cyminum l.) and cloves (syzygium aromaticum) essential oils, and their application to the preservation of minced meat(2018) Yahiaoui, Karima; Bouchenak, Ouahiba; Lefkir, S.; Benhabyles, N.; Laoufi, R.; Arab, KarimThe two plant species that were the subject of our study, namely cumin (Cuminum cyminum Cloves (Syzygium aromaticum), are frequently used in pharmacopoeia and culinary tradition. The antibacterial activities of these species were tested on pathogenic bacteria ( Salmonella Sp., Pseudomonas aeruginosa, oxaciline-sensitive Staphylococcus aureus refrigeration temperature. Based on the results, it can be predicted that the two essential oils studied are effective natural antimicrobial agents and can be used as a very important source of natural food preservatives