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    Valorization Of Brown Algae (cystoseira Caespitosa) From Local Region In Algeria For Sodium Alginate Extraction And Their Application In The Immobilization Of Microbial Pectinases
    (Université M'Hamed Bougara Boumerdes, 2019) Benmalek, N.; Nouani, A.; Benchabane, A.
    Sodium alginate were extracted from brown algae (Cystoseira Caespitosa) collected from Algerian Mediterranean coast. the physicochemical characteristic of sodium alginate showed that the average yield was 12% and the average molecular masse was 9000Da calculated using intrinsic viscosity data. Fourier transform infrared spectroscopy was used to characterize the extracted powder of sodium alginate, COO- and C-O-C were evident in the finger print region, the ratio of guluronic acid (G) / mannuronic acid (M) was recorded to be 0.91. This polysaccharide were used to immobilize crude pectinolytic enzymes synthesized by a strain of Aspergillus niger sp. The immobilization was carried out by dropwise introduction of alginate-enzyme mixture into a 0.2M CaCl 2 solution, by this technique, alginates shrinking in the form of spherical beads. The calcium alginate beads have been analyzed using scanning electron microscopy (SEM), observation under SEM showed that the beads have a rough surface and the enzymes were successfully included in the beads. The immobilization efficiency was about 83.9% and the immobilized enzyme could be reused in 4 cycles keeping 42,14% of its initial activity. The beads were successfully used for apple juice clarification.
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    Valorization of brown algae (Cystoseira caespitosa) from local region in Algeria for sodium alginate extraction and their application in the immobilization of microbial pectinases
    (Aljest, 2019) Hachemi-Benmalek, N.; Nouani, A.; Benchabane, A.
    Sodium alginate were extracted from brown algae (Cystoseira Caespitosa) collected from Algerian Mediterranean coast. the physicochemical characteristic of sodium alginate showed that the average yield was 12% and the average molecular masse was 9000Da calculated using intrinsic viscosity data. Fourier transform infrared spectroscopy was used to characterize the extracted powder of sodium alginate, COO- and C-O-C were evident in the finger print region, the ratio of guluronic acid (G) / mannuronic acid (M) was recorded to be 0.91. This polysaccharide were used to immobilize crude pectinolytic enzymes synthesized by a strain of Aspergillus niger sp. The immobilization was carried out by dropwise introduction of alginate-enzyme mixture into a 0.2M CaCl 2 solution, by this technique, alginates shrinking in the form of spherical beads. The calcium alginate beads have been analyzed using scanning electron microscopy (SEM), observation under SEM showed that the beads have a rough surface and the enzymes were successfully included in the beads. The immobilization efficiency was about 83.9% and the immobilized enzyme could be reused in 4 cycles keeping 42,14% of its initial activity. The beads were successfully used for apple juice clarification.
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    Biotechnology-2014 biodegradation performance of phenol by free and alginate entrapped cells of Haloarcula strain D21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria)
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Khemili, Souad
    Immobilization is a general term that describes many different forms of cell attachment or entrapment. These different forms include encapsulation of cells in a polymer-gel and entrapment in a matrix. The Advantages of these techniques are to reduced possibility of inoculum contamination during storage, transport and application. So, beads are non toxic, biodegradable and non-polluting and can be produced in large quantities, stored for extended periods. Moreover, phenols and phenolic compounds are widely distributed as environmental pollutants due to their common presence in the effluents of many industrial processes, including oil refineries, ceramic plants, coal conversion process, phenolic resins, pharmaceutical and food Industries. The aim of this study was to compare the biodegradation performance of phenol by using free and encapsulated cells of Haloarcula strain D21 isolated from crude oil contaminated saline water collected at Ain Salah in Algeria. Batch experiments were carried out in order to obtain the maximum phenol degradation rates by analyzing the influence of the immobilization in calcium-alginate gel beads on biodegradation performance