Optimization of biolipids production from Saccharomyces cerevisiae ATCC 9763 cultivated on Ampelodesmos mauritanicus (Diss) hydrolysate

Abstract

This study focuses on the production of biolipids by fermentation of the yeast Saccharomyces cerevisiae ATCC 9763 on a hydrolysate of the lignocellulosic biomass of Ampelodesmos mauritanicus (Diss). The central composite design (CCD) experimental plan was used to optimize biolipids production under the following experimental conditions: the concentration of dry yeast which varied from 3 to 12 g/L, the concentration of reducing sugars in the hydrolysate (glucose equivalent) was between 6 and 20 g/L, the C/N ratio varied from 40 to 180, and the fermentation time varied from 8 to 24 h. The optimal biolipid results were 49% obtained under conditions of 3 g/L of yeast concentration, 20 g/L of glucose concentration, 180 of C/N ratio, and 24 h of fermentation time. Biolipids were characterized by gas chromatography coupled with mass spectrometry (CG/MS) and the lipid profile revealed that palmitic fatty acid is the major component produced by yeast at a level of 48.49% followed by stearic fatty acid with a level of 43.65%; this fatty acid composition is similar to that of some vegetable oils. The promising potential of lignocellulosic biomass as a fermentation medium for Saccharomyces cerevisiae ATCC 9763 to produce fatty acids and other value-added compounds has been demonstrated and the produced biolipids are a promising feedstock for biodiesel. Graphical abstract: (Figure presented.)