Browsing by Author "Abderrahmani, Khaled"

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Effects of pollen deprivation in groups of tellian (Apis mellifera intermissa) and saharan (Apis mellifera sahariensis) honey bees under controlled conditions
(MDPI, 2022) Khedidji, Hassiba; Abderrahmani, Khaled; Oulebsir-Mohandkaci, Hakima; Ladjali-Mohammedi, Kafia; Mohammedi, Arezki
Worldwide, honey bees are increasingly faced with periods of pollen scarcity, which can lead to nutritional deficiencies, especially of proteins and amino acids. These are essential for the proper functioning of the single organism and the colony. To understand how bees react to protein deficiency, under controlled conditions, we studied the effect of pollen deficiency on the main physiological parameters in two subspecies endemic of Algeria, Apis mellifera intermissa and Apismellifera sahariensis. Emerging workers of both subspecies were reared with two diets: one was pollen-fed, whereas the other pollen-deprived. Several physiological criteria were measured depending on the type of diet and subspecies: the survival of the bees, the amount of total protein in the hemolymph, hypopharyngeal glands development and the ovary development of workers. These last three parameters were assessed at three different ages (7, 14 and 21 days). At birth, sahariensis workers weighed more than intermissa. With the same protein diet, the average life expectancy of sahariensis was extended by 5.55 days compared to intermissa. Even if deprived of pollen, sahariensis lived longer than intermissa fed with pollen (p < 0.001). In the three age levels, the hypopharyngeal glands were more developed and less affected by pollen deficiency (p < 0.001) in sahariensis than in intermissa (p < 0.001). The total hemolymph protein was higher in intermissa than in sahariensis regardless of the diet, and was also higher in protein-fed than in deprived bees (p < 0.001). The ovaries developed more rapidly with a high proportion in intermissa than in sahariensis (p < 0.05) regardless of the diet, and was also higher in the bees fed with pollen than those deprived (p < 0.05). Pollen deficiency generates physiological alterations and modifications, the amplitude of which varied according to the subspecies of the bee studied
Morphological and molecular identification of a new halotolerant cyanobacterial strain, Limnospira fusiformis TL03, isolated from Telamine Lake in the northwest of Algeria
(Sociedade Botanica do Brasil, 2025) Guenachi, Belkacem; Korteby Mefti, Hakima; Benfares, Redhouane; Abderrahmani, Khaled; Boudjema, Khaled; Achour, Hafsa Yaiche; Toumatia, Omrane; Bidin, Azmane; Lamari, Lynda
This study examines a new strain, Limnospira fusiformis TL03, isolated from Lake Telamine in northwest Algeria. The strain was morphologically identified using a light microscope and molecularly characterized using its 16S rRNA gene sequence. The effect of salinity on growth and photosynthetic pigments was studied using a spectrophotometric method; different sodium chloride concentrations (0, 5, 15, 30, 45, and 60 g/l) in blue-green medium (BG11) cultured uniformly as homogenized cell suspension were tested. A morphological examination confirmed that the isolated strain belonged to the Limnospira genus. It has trichome lengths ranging from 210-2027 µm, as well as pitch and coil diameters ranging from 30-137 µm and 20-60 µm, respectively. The data from the 16S rRNA gene sequence analysis confirmed that the isolated strain was Limnospira fusiformis TL03 with 100% sequence similarity to Limnospira fusiformis SAG 85.79. The results also indicate that this strain can grow at various salt concentrations, with the highest optical density values (1.58 ± 0.014 and 1.56 ± 0.003) obtained in cultures containing 15 g/L and 5 g/L NaCl, respectively.