Browsing by Author "Benfares, Redhouane"

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By-Products from the Processing of Canned African Catfish (Clarias gariepinus): Potential for the Production of Fishmeal, Collagen, and Gelatin
(Egyptian Society for the Development of Fisheries and Human Health, 2025) Kord, Affaf; Benfares, Redhouane; Boudjema, Kame; Bourafa, Imane; Benaziza, Amina; Aliouche, Faiza; Frai, Linda; Touzouirt, Saida
By-products generated during the canning process of African catfish (Clarias gariepinus), particularly heads and skins, represent a promising source of biologically and industrially valuable compounds. This study investigated the valorization of these by-products for the production of fishmeal, collagen, and gelatin. Biochemical analysis of fishmeal derived from the heads revealed high protein (48.42%) and ash (32.88%) contents. Collagen and gelatin extracted from the skins exhibited protein concentrations of 89.09% and 77.40%, respectively. Fourier Transform Infrared (FTIR) spectroscopy confirmed the characteristic structural features of these biomolecules, consistent with reference samples and previously reported data. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) of the gelatin extract showed protein bands with molecular weights ranging from 20 to 150 kDa, with α-type polypeptide chains identified between 100 and 150 kDa. These findings highlight the significant valorization potential of African catfish processing by-products and suggest promising applications in the pharmaceutical, food, and biomedical industries.
Evaluation of some biological activities of phenolic compounds obtained from two Algerian medicinal plants : mentha rotundifolia and satureja calamintha
(2020) Benfares, Redhouane; Boudjema, Khaled; Behlali, Hadjira; Imedjdouben, Imene; Kennas, Abderrezak; Fazouane, Fethia; Jaroslava, Švarc-Gajić
In this work phytochemical characterization of two medicinal plants from Lamiaceae family, Mentha rotundifolia and Satureja calamintha, has been carried out. Extracts obtained with different solvents were screened for different plant secondary metabolites and were biologically characterized by defining their antiradical and antibacterial activi-ties. Phytochemical screening of M. rotundifolia and S. calamintha confirmed their rich-ness in different secondary metabolites. The determination of phenolic compounds revea-led high polyphenols contents in water: methanol (30:70) extracts with concentrations of 20.64±1.74 mg EAG/g DW and 13.45±0.91 mg EAG/g DW for M. rotundifolia and S. calamintha, respectively. These extracts were also characterized by high concentrations of flavonoids (Mentha rotundifolia 12.33±1.58 mg EQ/g DW, Satureja calamintha 7.11±0.02 mg EQ/g DW). Furthermore, the water:methanol (30:70) extract of M. rotun-difolia was the most effective in inhibiting free radicals. Recorded inhibition diameters for both plant samples and tested microbial strains ranged from 6.66 mm to 13.66 mm. Presented results confirmed that tested indigenous Algerian plants are favorable sources of polyphenols with antioxidant and antimicrobial properties
Morphological and molecular identification of a new halotolerant cyanobacterial strain, Limnospira fusiformis TL03, isolated from Telamine Lake in the northwest of Algeria
(Sociedade Botanica do Brasil, 2025) Guenachi, Belkacem; Korteby Mefti, Hakima; Benfares, Redhouane; Abderrahmani, Khaled; Boudjema, Khaled; Achour, Hafsa Yaiche; Toumatia, Omrane; Bidin, Azmane; Lamari, Lynda
This study examines a new strain, Limnospira fusiformis TL03, isolated from Lake Telamine in northwest Algeria. The strain was morphologically identified using a light microscope and molecularly characterized using its 16S rRNA gene sequence. The effect of salinity on growth and photosynthetic pigments was studied using a spectrophotometric method; different sodium chloride concentrations (0, 5, 15, 30, 45, and 60 g/l) in blue-green medium (BG11) cultured uniformly as homogenized cell suspension were tested. A morphological examination confirmed that the isolated strain belonged to the Limnospira genus. It has trichome lengths ranging from 210-2027 µm, as well as pitch and coil diameters ranging from 30-137 µm and 20-60 µm, respectively. The data from the 16S rRNA gene sequence analysis confirmed that the isolated strain was Limnospira fusiformis TL03 with 100% sequence similarity to Limnospira fusiformis SAG 85.79. The results also indicate that this strain can grow at various salt concentrations, with the highest optical density values (1.58 ± 0.014 and 1.56 ± 0.003) obtained in cultures containing 15 g/L and 5 g/L NaCl, respectively.