Browsing by Author "Benyahia, Ibtissem"

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Characterization and Optimization of L-Asparaginase Production by Endophytic Fusarium sp3 Isolated From Malcolmia aegyptiaca of Southeast Algeria: Potential for Acrylamide Mitigation in Food Processing
(John Wiley and Sons Inc, 2025) Lakhdari, Wassima; Neghmouche Nacer, Salah; Benyahia, Ibtissem; Hammi, Hamida; Bachir, Hakim; Mouhoubi, Djawahir; Lakhdari, Yasmine; Guemmou, Ihsane; Dehliz, Abderrahmene; Sawicka, Barbara
This study aims to isolate and optimize the production of L-asparaginase from fungal strains derived from Algerian Saharan plants, and evaluate the reduction of acrylamide formation in food products. L-asparaginase has frequently been used to treat childhood acute lymphoblastic leukemia. It catalyzes the hydrolysis of asparagine and glutamine into aspartic acid and ammonia. It is also used in the food industry to minimize acrylamide formation during high-temperature frying of starchy food items. In this study, L-asparaginase was identified in various microbial, animal, and plant species. Using Czapek-Dox medium, different fungal species were first isolated from Saharan plants of southeast Algeria (including Zygophyllum cornutum Coss., Malcolmia aegyptiaca Spreng., Phoenix dactylifera L., and Cyperus rotundus L.) and tested for their ability to produce extracellular L-asparaginase. Among 13 isolates, nine were positive in the preliminary test. The strain Fusarium sp.₃, isolated from M. aegyptiaca leaves, had the highest enzyme index (1.92 ± 0.35) with maximum enzyme production (63.68 units per milliliter). Critical factors such as temperature (30°C–50°C), pH (5.0–8.0), and substrate concentration (1–10 g/L) were optimized under liquid-state fermentation to maximize enzyme production. Utilizing Minitab software, additional statistical methods were employed for the optimization process, including the Plackett–Burman design and response surface methodology. The Plackett–Burman design screened seven variables: temperature, pH, incubation time, substrate concentration, glucose concentration, nitrogen source, and agitation speed. The design identified asparagine concentration, incubation time, and pH as the most significant factors for asparaginase production. Response surface methodology was then used to optimize these factors, producing maximum asparaginase in a 50-mL medium. Under optimized conditions, the application of L-asparaginase to potato slices prior to frying resulted in a 68% reduction in acrylamide content (from 435.6 ± 12.4 μg/kg to 139.3 ± 8.7 μg/kg), demonstrating the enzyme's strong potential for improving food safety
Chemical composition and bioactivity of essential oil against the green peach aphid (Myzus persicae)
(Springer, 2022) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Chahbar, Nora; Acheuk, Fatma; Mekhadmi, Nour El Houda; Benyahia, Ibtissem; Fethallah, Rabab; Hammi, Hamida; Berrekbia, Mohammed; Badjadi, Zeineb
The present experiment was conducted to elucidate the chemical composition of essential oil (EO) of a native spontaneous plant, Cotula cinerea Del., in southeastern Algeria and to evaluate its insecticidal effect on the Green Peach Aphid (GPA) Myzus persicae Sulzer. GC–MS and GC-FID analyses showed that this plant was characterized by 35 components, representing eight major constituents, i.e., santolina triene (11.55%), sabinene (5.86%), cineol (6.21%), α-thujone (22.91%), (R)-camphor (7.50%), cis-sabinyl acetate (6.41%), carveol (7.68%), and ipsenol (4.60%). The considered essential oil was characterized by a clear predominance of hydrocarbon compounds chiefly monoterpenes (35.63%), oxygenated monoterpenes (64%), and sesquiterpenes (0.35%). Insecticidal assays showed that the EO was toxic against M. persicae adults recording 75.95% of mortality in the 3rd dose with a lethal concentration (LC50) equal to 302.4 µl where the lethal time (LT50) was 91.6 h. Also, it has a significant repellency showing a high rate in the 2nd dose (64.6%) with a highly significant difference (p = 0.000). As well as its inhibitory effect on the egg-laying where it has greatly reduced the reproduction potential of M. persicae adults. Obtained data support the possibility to develop C. cinerea oil, especially component specification, as a new natural bio-insecticide
Chemical composition and insecticidal activity of Artemisia absinthium L. essential oil against adults of Tenebrio molitor L.
(Elsevier Ltd, 2024) Lakhdari, Wassima; Mounir Bouhenna, Mustapha; Salah Neghmouche, Nacer; Dehliz, Abderrahmene; Benyahia, Ibtissem; Bendif, Hamdi; Garzoli, Stefania
Many researchers and manufacturers around the world have shown interest in essential oils and their components. Artemisia absinthium L., commonly known as wormwood, is a medicinal and aromatic bitter herb frequently used in traditional medicine since ancient times. The objective of this research was to study the chemical composition and evaluate the insecticidal activities of A. absinthium essential oil (AEO) against Tenebrio molitor L., mealworm battle. We explored its effectiveness through several means, including direct contact, fumigation and repulsion of insects. Phytochemical analysis was performed using GC-MS technique. The main components identified in the essential oil were camphor (32.34%), chamazulene (13.92%), and terpinen-4-ol (10.18%). These results highlight that Algerian A. absinthium plants are valuable resources due to their bioactive compounds. In particular, the results obtained in our investigation suggest that the essential oil obtained from A. absinthium has the potential to be used as a natural bioinsecticide.
Chemical compositions and insectisidal activity of essential oils of three plants Artemisia SP: Artemisia herba-alba, Artemisia absinthium and Artemisia Pontica (Morocco)
(Elsevier, 2024) Lakhdari, Wassima; Mounir Bouhenna, Mustapha; Salah Neghmouche, Nacer; Dehliz, Abderrahmene; Benyahia, Ibtissem; Bendif, Hamdi; Garzoli, Stefania
Many researchers and manufacturers around the world have shown interest in essential oils and their components. Artemisia absinthium L., commonly known as wormwood, is a medicinal and aromatic bitter herb frequently used in traditional medicine since ancient times. The objective of this research was to study the chemical composition and evaluate the insecticidal activities of A. absinthium essential oil (AEO) against Tenebrio molitor L., mealworm battle. We explored its effectiveness through several means, including direct contact, fumigation and repulsion of insects. Phytochemical analysis was performed using GC-MS technique. The main components identified in the essential oil were camphor (32.34%), chamazulene (13.92%), and terpinen-4-ol (10.18%). These results highlight that Algerian A. absinthium plants are valuable resources due to their bioactive compounds. In particular, the results obtained in our investigation suggest that the essential oil obtained from A. absinthium has the potential to be used as a natural bioinsecticide.