Browsing by Author "Bourfis, Nassima"

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    Adsorption kinetics modelling of methylene blue solution by activated carbon using Peleg’s equation
    (Université M'hamed Bougara de Boumerdès, 2020) Bourfis, Nassima; Benaissa, F.; Benamara, S.; Gougam, H.; Louhab, K.
    The aim of this study is to envisage the possibility of modelling the adsorption kinetics of methylene blue (MB) by activated carbon using the Peleg’s model. Equation often used to describe the sorption and absorption kinetics of water by the material. The study of equilibrium adsorption was also envisaged in this study and the isotherms of Langmuir and Freundlich were tested. The activated carbon was prepared from almond shells bitter, fruit of plants growing in parts of Algeria, especially in the Setif region. Statistical analyses, have proved that the adsorption kinetics of MB by activated carbon is perfectly (R² = 0.9999) described by the Peleg model. On another hand, the study showed that, compared to the Langmuir model, Freundlich model is most appropriate to describe the equilibrium adsorption
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    Characterization and antibacterial activity of alkaloids and polyphenols extracts from Haplophyllum tuberculatum (Forssk.) A. Juss.
    (Pamukkale University, 2023) Djouahra-Fahem, Djamila; Bensmail, Souhila; Bouteldja, Razika; Messad, Sara; Ferhoum, Fatiha; Bourfis, Nassima; Acheuk, Fatma; Fazouane, Fethia
    Haplophyllum tuberculatum is a medicinal plant belonging to the Rutaceae family, is renowned for its various therapeutic properties. This study aims to characterize the alkaloids and polyphenol extracts from this plant and assess their antibacterial activity. Herein, the extraction of polyphenols and alkaloids from this plant was performed by the maceration method. Folin Ciacalteu's method was used to estimate the total phenolic content, and the qualitative characterization of the two extracts was performed by thin-layer chromatography. Whilst, the antibacterial activity of the two extracts was tested with the disk diffusion method on a solid medium and the minimal inhibitory concentration (MIC) of susceptible bacteria was determined using the agar dilution method. Our results indicate respective yields of 8.39% in polyphenols and 0.37% in alkaloid extracts, while the total phenolic content was estimated to be 74.45 mg GAE/g of dry matter in polyphenolic extract. Thin-layer chromatography analysis allowed choosing the system toluene-acetate-ethanol-concentrated ammonia (40:4:8:3, v/v) to separate H. tuberculatum alkaloids, and ethyl acetate-methanol-water (100:13.5:10, v/v/) for the separation of its polyphenols. The same analysis detected traces of quercitin, catechin, and rutin in the polyphenolic extract. Our findings demonstrated good antibacterial activity on Gram-positive strains such as Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633, and moderate activity on Pseudomonas aeruginosa ATCC 27953, with MICs ranging from 0.625 to 10 mg/mL for alkaloids and from 5 to 20 mg/mL for polyphenols.
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    Factors affecting acid protease production by Mucor circinelloides MG603064.1 through SmF process: characterization and fromage frais making
    (BioTechnologia, 2023) Bensmail, Souhila; Boudjema, Khaled; Naimi-Fazouane, Fethia; Bensmail, Samira; Djouahra-Fahem, Djamila; Ferhoum, Fatiha; Bourfis, Nassima
    The exploitation of food industry wastes and their conversion into value-added products present a promising and continuously growing field, given the diversity of elaborated wastes. The current work aimed to utilize sweet cheese whey as a growth medium for acid protease production by a local fungus strain. The biochemical and physicochemical properties of the cheese whey, such as pH, conductivity, chemical oxygen demand, biological oxygen demand (BOD5), total nitrogen and protein contents, and mineral salts, were assessed using various analytical methods. The effect of certain parameters on acid protease production by Mucor circinelloides MG603064.1 through the SmF process was investigated using the conventional design method "One factor at a time". Subsequent to characterization, the crude extract was used in a trial to create fromage frais, compared to the commercial rennin CHY-MAX® Powder Extra. Cheese whey characterization revealed its richness in total nitrogen (1.044 ± 0.044 g/l), protein content (6.52 ± 0.04 g/l), and principal mineral salts: calcium (1.637 ± 0.037 g/l), phosphorus (1.173 ± 0.023 g/l), and chloride (1.66 ± 0.09 g/l). The optimal values of the SmF process for acid protease production, such as the inoculum size, beef extract, and KH2PO4 supplements, the initial pH of cheese whey, and incubation temperature were, respectively, 11% (v/v), 0.4% (w/v), 0.5% (w/v), 5.5, and 30°C. Under these conditions, the lowest milk-clotting time of 290 s was achieved, representing an 18.41-fold increase compared to the initial step using the unoptimized medium. The enzyme showed maximum milk-clotting activity at pH 5, a temperature of 60°C, and in the presence of 0.025 M of CaCl2. The enzyme activity also significantly improved with sonication (35 kHz) for 10 min. The crude extract of M. circinelloides ensured the production of fresh cheese samples with characteristics roughly similar to those obtained by the control (CHY-MAX® rennin). The acid protease of M. circinelloides could successfully substitute the conventional rennin in the manufacture of fresh cheese.
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    Valorisation de la coquille d’amande amère issue de la région d’Ait Ourthilène (Sétif) : comme un adsorbant des colorants
    (Université M'Hamed Bougara : Faculté de Technologie, 2021) Bourfis, Nassima; Louhab, Krim(Directeur de thèse)
    La presente etude a pour objectif d fetudier un nouvel adsorbant naturel biodegradable a base de la coquille d famande amere (Prunus amygdalus), espece qui pousse spontanement dans la region de Setif (Nord-est algerien). Les essais d fadsorption realises sur le bleu de methylene (BM), qui est un colorant cationique, ont montre une elimination remarquable des les 20 premieres minutes. Une gamme d fanalyses physico-chimiques a ete effectuee dont : la fluorescence X, infra-rouge a transformee de Fourrier, microscope electronique a balayage, determination de la surface specifique par la methode de Brunauer, Emett et Teller et l fanalyse thermique gravimetrique. L fadsorption du bleu de methylene par la coquille d famande a ete etudiee, en premier lieu en systeme batch, en evaluant les effets du pH de la solution, de la dose d fadsorbant, de la concentration initiale du melange, du temps de contact, et de la temperature sur le phenomene d fadsorption, via la methode des plans d fexperiences. Les resultats experimentaux ont montre que le rendement d fadsorption du colorant d finteret sur la coquille d famande a atteint 97,36% a une concentration initiale du BM = 150 mg/l, pH =13, T ‹ = 30 ‹C avec une dose de la CA = 1g/l. La cinetique d fadsorption a ete verifiee en testant plusieurs modeles cinetiques afin de determiner le mecanisme de biosorption. Il a ete determine que le systeme de biosorption suit une loi cinetique d fordre 2 en tenant compte de la valeur du coefficient de correlation R qui etait superieur a 0,99. L fetude de l fisotherme a montre que le modele de Freundlich decrit bien le processus de l fadsorption du bleu de methylene par la coquille d famande amere. Les parametres thermodynamiques ont ete calcules: ƒ¢H, ƒ¢S, et ƒ¢G. Le processus d'adsorption est avere etre endothermique et spontanee. L fadsorption du bleu de methylene en colonne a montre que l faugmentation du debit d falimentation a un effet negatif sur l fadsorption alors qu fune concentration initiale plus grande favorise l fadsorption ce qui est en concordance avec les resultats obtenus en systeme batch. Les experiences executees confirment qu fil est tres possible d fobtenir un adsorbant actif a partir de la coquille, par activation chimique (acide ou alcaline) et sans recours au procede de carbonisation
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