Browsing by Author "Boutiche, Malika"

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    Caractérisation physico-chimique et microbiologique des grignons d'olives de quelques huileries en Algérie et essai de culture d'un microchampignon hyphomycète en vue de production d'enzymes
    (Université M'Hamed Bougara Boumerdès : Faculté des Sciences, 2024) Boutiche, Malika; Sahir-Halouane, Fatma(Directeur de thèse)
    La présente étude a pour objectif de caractériser (propriétés physico-chimiques et microbiologiques) des grignons d'olives provenant de quatre régions : Jijel, Bejaia, Tizi-Ouzou et Bouira, et de valoriser cette biomasse ligno-cellulosique en tant que substrat de culture pour la production de cellulases, en utilisant le champignon de Trichoderma reesei RUT C-30. Les résultats de la caractérisation physico-chimique ont montré que les grignons d'olive sont riches en fibres NDF (46,33% à 58,74%). La caractérisation microbiologique a révélé que ces sous-produits peuvent être un milieu très favorable à la croissance et le développement de T. reesei RUT C30. Ces grignons ont été colonisés principalement par la flore mésophiles aérobies totales et les levures et les moisissures dont la charge a varié de 0,45×105 à 1,36×107 UFC/ml et de 2×105 à 4,76×106 UFC/ml, respectivement. Parmi les quatre grignons testés, les grignons bruts de la région de Jijel ont révélé la meilleure production de cellulases (activité papier filtre = 0,78U/gSS). L'effet des trois traitements (alcalin, broyage et thermique) et de leurs combinaisons sur la composition physico-chimique et sur la production de cellulases a été étudié. Les résultats ont montrés que la composition chimique a été significativement affectée (P <0,05) par les différents traitements ainsi que leurs combinaisons. Le traitement alcalin (1% NaOH) des grignons a amélioré l'activité papier filtre à une valeur de 1,28UI / gSS, tandis que des rendements inférieurs ont été obtenus avec les traitements de broyage (0,2UI / gSS) et thermique (0,15UI / gSS). La modification des conditions de culture (épuisement des grignons à l'acétone) a montré une amélioration remarquable de l'activité papier filtre (2,63 UI/gSS) par rapport à celles enregistrés par les grignons non traités. Les résultats de l'analyse SDS-PAGE révèlent que l'extrait enzymatique brut contient deux complexes enzymatiques différents de cellulases et xylanases
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    Characterization and valorization of olive pomace for production of cellulase from Trichoderma reesei RUT C30 in solid-state fermentation
    (ALJEST, 2020) Boutiche, Malika; Sahir-Halouane, F.; Meziant, L.; Boulaouad, I.; Saci, F.; Fiala, S.; Bekrar, A.; Mesbah, R.; Abdessemed, A.
    The aim of the present work is to characterize the olive pomace (physico-chemical and microbiological properties) of four regions known by their high production of olive oil in the north of Algeria and to valorize the lignocellulosic biomass as culture substrate for cellulases production using Trichoderma reesei RUT C-30. The results obtained show that the olive pomace is a favorable environment for the growth of fungi. The load of total aerobic mesophilic flora, yeasts and molds varies from 0.45×106 to 1.36×108 CFU/g DM and from 2×106 to 4.76×107 CFU/g DM, respectively. Physico-chemical parameters of the four regions revealed significant differences (p<0.05). The determination of filter paper activities of enzymatic extracts reveals that olive pomace from Jijel presented the best activity (0.78 U/gds).
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    Effect of exhaustion of olive pomace on cellulases production from Trichoderma reesei RUT C30 and identification of the enzyme complexes
    (Springer, 2024) Boutiche, Malika; Bennacer, Amel; Aissaoui, Mohammed; Meziant, Leila; Saci, Fairouz; Siar, El-Hocine; Boulaouad, Ilyes; Bekrar, Amel; Fiala, Samah; Hemoui, Hadjer; Sahir-Halouane, Fatma
    Olive industry generates large quantities of solid by-products such as olive pomace. The objective of present work is to study the effect of exhaustion (residual oil extraction) by acetone solvent on the improvement of cellulase production using olive pomace as substrate, and to identify the crude enzymatic extract by electrophoretic analysis. The production of cellulases was done by the solid fermentation process using Trichoderma reesei RUT C30 as cellulolytic fungus grown on olive pomace. Cellulolytic activity was assessed by the filter paper method (FPase). Electrophoretic analysis by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was performed for the identification of enzymatic extract of T. reesei RUT C30. Chemical composition (lipids, proteins, sugars and ash) was significantly (P < 0.05) affected after olive pomace exhaustion. In general, exhaustion by acetone solvent increased the fiber fraction. Exhaustion improved the results of FPase activity compared to those obtained on untreated substrate, giving values of 2.63 IU/gds and 1.1 IU/gds, respectively. The results of SDS-PAGE analysis revealed that the crude enzymatic extract contains two different enzyme complexes including cellulases and xylanases.
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    Effect of pretreatments on physicochemical Characteristics of Olive Pomace and on Production of cellulases from trichoderma reesei RUT C30 under solid-state fermentation
    (2023) Boutiche, Malika; Sahir-Halouane, Fatma; Meziant, Leila; Saci, Fairouz; Oudjedi, Kahina; Derdour, Mouna; Ouffroukh, Karima; Maghboune, Ibtissem; Fiala, Samah; Bekrar, Amel
    Olive pomace (OP) is a cheap and abundant agricultural by-product that could be valorized by different biotechnological processes. The present study was conducted to better understand the effects of alkaline, milling and thermal pretreatments on OP for obtaining high value-added products (cellulases). Trichoderma reesei RUT C-30 fungus was used for cellulases production on OP substrate under Solid-State Fermentation (SSF) process and cellulases activity was assessed by the filter paper method (FPase). The effect of the three pretreatments and their combinations on physico-chemical composition and cellulases production was investigated. Results showed that untreated OP was a favorable environment for the growth of T. reesei and a good fermentation substrate that gave FPase activity of 0.83 IU/g DS. The chemical composition (lipids, proteins, carbohydrates and ash) was significantly (P < 0.05) affected by the different pretreatments as well as their combinations. Regarding the fiber fraction, alkaline and thermal pretreatments did not affect the NDF content, while a remarkable decrease (29.88%) was recorded after milling pretreatment. Alkaline pretreatment decreased significantly the cellulose contents whereas milling increased it of 35%. ADL fraction was only decreased by the milling treatment of 42%. No significant effect of thermal pretreatment was noticed on ADL and cellulose. The alkaline pretreatment with 1% NaOH improved the cellulase activity to a value of 1.28 IU/g DS, while lower yields were obtained after milling (0.2 IU/g DS) and thermal (0.15 IU/g DS) pretreatments. This study showed that only alkaline treatment improved the production of cellulase from OP without being combined with milling and thermal treatment