Browsing by Author "Dehliz, Abderrahmene"

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Agro-morphological response of corn (zea mays l.) to a biostimulant from the southeastern Algeria
(2020) Lakhdari, Wassima; Dehliz, Abderrahmene; Mlik, Randa; Chergui, Salima; Benlamoudi, Wiam; Lakhdari, Fatma; Acheuk, Fatma; Hammi, Hamida
This study highlights the effect of a biostimulant based on Trichoderma, indigenous antagonistic fungus from the region of Touggourt, on the development of a local variety of corn. This study was conducted at the experimental station of National Institute of Agronomic Research (Sidi Mehdi, Touggourt) as a complete randomaize block designe. The examined agro-morphological parameters showed that the application of this bioproduct stimulates vegetative growth of the plant. Besides, better germination and earliness of seeds. The results showed more interesting values were obtained in the treated plots (height = 135.9 cm, diameter = 28.9 mm, surface of leaves = 318 cm2, Pan = 10.2 panicles/plant) compared to those of the controls (height = 98.6 cm, diameter = 23.7 mm, surface of leaves = 260.4 cm², Pan = 7.4 panicles/plant). Statistical analyzes confirmed the existence of a very highly significant difference between treated and control subjects
Characterization and Optimization of L-Asparaginase Production by Endophytic Fusarium sp3 Isolated From Malcolmia aegyptiaca of Southeast Algeria: Potential for Acrylamide Mitigation in Food Processing
(John Wiley and Sons Inc, 2025) Lakhdari, Wassima; Neghmouche Nacer, Salah; Benyahia, Ibtissem; Hammi, Hamida; Bachir, Hakim; Mouhoubi, Djawahir; Lakhdari, Yasmine; Guemmou, Ihsane; Dehliz, Abderrahmene; Sawicka, Barbara
This study aims to isolate and optimize the production of L-asparaginase from fungal strains derived from Algerian Saharan plants, and evaluate the reduction of acrylamide formation in food products. L-asparaginase has frequently been used to treat childhood acute lymphoblastic leukemia. It catalyzes the hydrolysis of asparagine and glutamine into aspartic acid and ammonia. It is also used in the food industry to minimize acrylamide formation during high-temperature frying of starchy food items. In this study, L-asparaginase was identified in various microbial, animal, and plant species. Using Czapek-Dox medium, different fungal species were first isolated from Saharan plants of southeast Algeria (including Zygophyllum cornutum Coss., Malcolmia aegyptiaca Spreng., Phoenix dactylifera L., and Cyperus rotundus L.) and tested for their ability to produce extracellular L-asparaginase. Among 13 isolates, nine were positive in the preliminary test. The strain Fusarium sp.₃, isolated from M. aegyptiaca leaves, had the highest enzyme index (1.92 ± 0.35) with maximum enzyme production (63.68 units per milliliter). Critical factors such as temperature (30°C–50°C), pH (5.0–8.0), and substrate concentration (1–10 g/L) were optimized under liquid-state fermentation to maximize enzyme production. Utilizing Minitab software, additional statistical methods were employed for the optimization process, including the Plackett–Burman design and response surface methodology. The Plackett–Burman design screened seven variables: temperature, pH, incubation time, substrate concentration, glucose concentration, nitrogen source, and agitation speed. The design identified asparagine concentration, incubation time, and pH as the most significant factors for asparaginase production. Response surface methodology was then used to optimize these factors, producing maximum asparaginase in a 50-mL medium. Under optimized conditions, the application of L-asparaginase to potato slices prior to frying resulted in a 68% reduction in acrylamide content (from 435.6 ± 12.4 μg/kg to 139.3 ± 8.7 μg/kg), demonstrating the enzyme's strong potential for improving food safety
Chemical composition and bioactivity of essential oil against the green peach aphid (Myzus persicae)
(Springer, 2022) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Chahbar, Nora; Acheuk, Fatma; Mekhadmi, Nour El Houda; Benyahia, Ibtissem; Fethallah, Rabab; Hammi, Hamida; Berrekbia, Mohammed; Badjadi, Zeineb
The present experiment was conducted to elucidate the chemical composition of essential oil (EO) of a native spontaneous plant, Cotula cinerea Del., in southeastern Algeria and to evaluate its insecticidal effect on the Green Peach Aphid (GPA) Myzus persicae Sulzer. GC–MS and GC-FID analyses showed that this plant was characterized by 35 components, representing eight major constituents, i.e., santolina triene (11.55%), sabinene (5.86%), cineol (6.21%), α-thujone (22.91%), (R)-camphor (7.50%), cis-sabinyl acetate (6.41%), carveol (7.68%), and ipsenol (4.60%). The considered essential oil was characterized by a clear predominance of hydrocarbon compounds chiefly monoterpenes (35.63%), oxygenated monoterpenes (64%), and sesquiterpenes (0.35%). Insecticidal assays showed that the EO was toxic against M. persicae adults recording 75.95% of mortality in the 3rd dose with a lethal concentration (LC50) equal to 302.4 µl where the lethal time (LT50) was 91.6 h. Also, it has a significant repellency showing a high rate in the 2nd dose (64.6%) with a highly significant difference (p = 0.000). As well as its inhibitory effect on the egg-laying where it has greatly reduced the reproduction potential of M. persicae adults. Obtained data support the possibility to develop C. cinerea oil, especially component specification, as a new natural bio-insecticide
Chemical composition and insecticidal activity of Artemisia absinthium L. essential oil against adults of Tenebrio molitor L.
(Elsevier Ltd, 2024) Lakhdari, Wassima; Mounir Bouhenna, Mustapha; Salah Neghmouche, Nacer; Dehliz, Abderrahmene; Benyahia, Ibtissem; Bendif, Hamdi; Garzoli, Stefania
Many researchers and manufacturers around the world have shown interest in essential oils and their components. Artemisia absinthium L., commonly known as wormwood, is a medicinal and aromatic bitter herb frequently used in traditional medicine since ancient times. The objective of this research was to study the chemical composition and evaluate the insecticidal activities of A. absinthium essential oil (AEO) against Tenebrio molitor L., mealworm battle. We explored its effectiveness through several means, including direct contact, fumigation and repulsion of insects. Phytochemical analysis was performed using GC-MS technique. The main components identified in the essential oil were camphor (32.34%), chamazulene (13.92%), and terpinen-4-ol (10.18%). These results highlight that Algerian A. absinthium plants are valuable resources due to their bioactive compounds. In particular, the results obtained in our investigation suggest that the essential oil obtained from A. absinthium has the potential to be used as a natural bioinsecticide.
Chemical compositions and insectisidal activity of essential oils of three plants Artemisia SP: Artemisia herba-alba, Artemisia absinthium and Artemisia Pontica (Morocco)
(Elsevier, 2024) Lakhdari, Wassima; Mounir Bouhenna, Mustapha; Salah Neghmouche, Nacer; Dehliz, Abderrahmene; Benyahia, Ibtissem; Bendif, Hamdi; Garzoli, Stefania
Many researchers and manufacturers around the world have shown interest in essential oils and their components. Artemisia absinthium L., commonly known as wormwood, is a medicinal and aromatic bitter herb frequently used in traditional medicine since ancient times. The objective of this research was to study the chemical composition and evaluate the insecticidal activities of A. absinthium essential oil (AEO) against Tenebrio molitor L., mealworm battle. We explored its effectiveness through several means, including direct contact, fumigation and repulsion of insects. Phytochemical analysis was performed using GC-MS technique. The main components identified in the essential oil were camphor (32.34%), chamazulene (13.92%), and terpinen-4-ol (10.18%). These results highlight that Algerian A. absinthium plants are valuable resources due to their bioactive compounds. In particular, the results obtained in our investigation suggest that the essential oil obtained from A. absinthium has the potential to be used as a natural bioinsecticide.
Inhibitory effect of Trichoderma harzianum on mycelial growth of Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani
(Springer Netherlands, 2018) Lakhdari, Wassima; Dehliz, Abderrahmene; Mlik, Randa; Hammi, Hamida; Benlamoudi, Wiam; Acheuk, Fatma; Doumandji-Mitiche, Bahia B.
Tomato is one of the most important fruit vegetables for human consumption in Algeria. In recent years, the cultivation of this plant is experiencing a very rapid development. However, this speculation is confronted with several problems including plant health. The purpose of this study is to find biological ways to introduce them into an integrated control program against tomato pests and have an organic agriculture that can increase the production of this culture. Direct confrontation test of an indigenous strain of Trichoderma harzianum with two phytopathogenic fungi that are isolated from tomato leaves (Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani) was carried at 26 °C on an artificial culture medium (PDA). It could be demonstrated that T. harzianum inhibited mycelia growth of the two fungi with more than 40% and only after 4 days of incubation. Statistical analysis confirms that T. harzianum had a significant effect of antagonism against the tested pathogens mentioned before with P value 0.02 and 0.04, respectively. Beyond this period, and at the end of the sixth day, T. harzianum occupied the colonies of fungus and began to sporulate marking a very important myco-parasitic power. Indeed, a percent inhibition greater than 50% was recorded into the both fungi
Phytochemical screening, antioxidant, antibacterial, and antifungal properties of the Cymbopogon citratus methanolic extract
(Elsevier, 2025) Nacer, Salah Neghmouche; Wassima, Lakhdari; Boussebaa, Walid; Abadi, Abderrezzak; Benyahia, Ibtissem; Mouhoubi, Djawahir; Ladjal, Amel; Hammi, Hamida; Bachir, Hakim; Dehliz, Abderrahmene
In this work, the aerial parts of Cymbopogon citratus, harvested in Algeria, were analyzed as potential sources of biologically active compounds. The phytochemical composition, the content of total phenols and flavonoids in the methanolic extract of this plant, as well as its antioxidant, antibacterial, and antifungal capacities, were evaluated. The methanolic extract had phenolic compounds and flavonoids, with levels of 92.82 µg GAE/mg Dry weight and 58.98 µg QE/mg Dry weight, respectively. Furthermore, the methanolic extract exhibited moderate antioxidant activity as assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′azinobis (3-ethylbenzothiozoline-6-sulfonic acid) disodium salt radical), and β-carotene tests, while its antimicrobial activity was tested against four bacterial strains. Thus, a MBC/MIC ratio of 2 indicates that the extract exhibits bactericidal activity against Escherichia coli. Additionally, the extract exhibited antifungal activity against Thielaviopsis paradoxa, Alternaria sp., and Fusarium solani, with inhibition rates ranging from 50.59 % to 70.20 %. LC-MS analysis identified a variety of components, including chlorogenic acid (17.40 %), catechin (34.64 %), salicin (9.55 %), and curcumin (8.97 %), which are likely responsible for the observed activities. This study demonstrated that the aerial parts of Cymbopogon citratus offer a cost-effective source of natural compounds with bactericidal and antifungal activity, offering potential applications in the food and pharmaceutical industries
Potential of Zygophyllum album L. to control Tuta absoluta in Southeastern Algeria
(Springer, 2020) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Hammi, Hamida; Guezoul, Omar; Acheuk, Fatma; Benlamoudi, Wiam; Chergui, Salima; Guermit, Keltoum; Matallah, Salim; Berrekbia, Mohammed; Gheriani, Sofiane
On the valorization of spontaneous plants to contribute to organic agriculture, a biological study was undertaken by using the aqueous extract of Zygophyllum album (family of Zygophyllaceae), collected from Southeastern Algeria. Three levels of treatment (100%, 50%, and 25%) diluted from the extracted solution of this plant were tested on larvae (IN = 30) and adults (IN = 30) of tomato borer Tuta absoluta. This work reveals larval mortality rates of 7.5 ± 0.58% after 24 h, 12.50 ± 2.38% after 48 h, 30 ± 4.08% after 72 h, 40 ± 4.08% after 96 h, and 42.5 ± 6.45% after 120 h, although, with 50% of the crude extract, values of 20 ± 5.77%, 40 ± 4.08%, 50 ± 4.08%, 65 ± 5.77%, and 70 ± 4.58% were recorded at the same test durations, respectively. When a 25% dose of this solution was used, a mortality rate of 35 ± 8.16% was noted after 24 h and exceeds double (77.5 ± 8.66%) in 120 h. These data show that the toxicity of Z. album aqueous extract against T. absoluta increases with time and from the highest dose to the smallest one. Females of T. absoluta lay only 10 ± 3.54 to 19 ± 3.61 eggs/leaflet on treated tomato leaves, but a double amount was recorded on the control (37.25 ± 13.15 eggs/leaflet). This observation shows a repulsive effect of Z. album against T. absoluta females