Browsing by Author "Halouane, Fatma"

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    Effect of entomopathogenic fungi beauveria bassiana and metarhizium anisopliae var. acridum on the haemolymph of the desert locust schistocerca gregaria
    (2011) Milat-Bissaad, Fatma Zohra; Bounaceur, Farid; Halouane, Fatma; Behidj, Nassima; Chebouti, Nadjiba; Doumandji-Mitiche, Bahia
    Effect of Beauveria bassiana and Metarhizium anisopliae var. acridum on the 5 th instar nymphs of Schistocerca gregaria was studied in the laboratory. Infection by these both entomopathogenic fungi caused reduction in the hemolymph total protein. Th e average amounts of total proteins were 2.3, 2.07, 2.09 μg/100 ml of haemolymph in the control and M. anisopliae var. acridum , and B. bassiana based- treatments, respectively. Three types of haemocytes were recognized and identified as prohaemocytes, plasmatocytes and granulocytes. The treatment cause d significant reduction in the total haemocyte count and in each haemocyte type on the 9 th day after its application
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    Etude de l’effet de deux champignons entomopathogenes Beauveria bassiana et Metarhizium anisopliae var acridum sur le comportement alimentaire de Schistocerca gregaria
    (2011) Milat-Bissaad, Fatma Zohra; Bounaceur, Farid; Halouane, Fatma; Outtar, Fahima; Doumandji-Mitiche, Bahia
    Dans le présent travail il est noté que les deux champignons entomopa thogènes Beauvaria bassiana et Metarhizium anisopliae var acridum, traités par ingestion ont affecté l’évolution pondérale des larves L 5 de Schistocerca gregaria . L’examen des résultats montre de même que le gain de poids moye n après 9 jours de traitement chez les larves du lot témoin est de 0,4230g. Il est de 0,159g pour celles traitées au B. bassiana . Par contre chez les larves traitées avec le M. anisopliae leur gain de poids est très réduit, il est de 0,011g. Concernant la consommation initiale des L 5 de S. gregaria , elle débute au 1 er jour avec 0,716±0,086g pour les témoins contre 0,05±0,008g et 0,691±0,083g respectivement chez les traitées au B. bassiana et au M. anisopliae . Cette consommation augmente et diminue au cours du temps et atteint des valeurs soit supérieures ou inférieures aux valeurs initiales jusqu'à atteindre finalement au 9 ème jour 0,168±0,020g, 0,029±0,005g et 0,068±0,008g respectivement pour les témoins et les traitées au B. bassiana et au M. anisopliae . Les indices nutritionnels de consommation et d’utilisation de la nourriture Coefficient d’Utilisation Di gestive apparent (CUD a ), l’Efficacité de Conversion de la nourriture Digérée en matière corporelle (ECD) et l’Efficacité de Conversion de la nourriture Ingérée en matière corporelle (ECI) ont été aussi affectés par le traitement aux deux cryptogames
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    Histological changes in the larvae of the domestic mosquito Culex pipiens treated with the entomopathogenic fungus Beauveria bassiana
    (Academic Journals, 2017) Benzina, Farida; Hamid, Sonia; Mohand-Kaci, Hakima; Bissaad, Fatma; Halouane, Fatma
    The Culicidae are biting insects that are most harmful to people. They are almost all blood-suckers, and are responsible for the spread of many important diseases such as malaria, yellow fever and elephantiasis. Entomopathogenic microorganisms occupy an important place among the alternative methods of fighting against pests insect. The fungus, Beauveria bassiana is an entomopathogenic agent naturally present in the ecosystems and offers a very interesting potential for controlling populations of mosquitoes. Our study aims at showing the 4th stage larvae histological changes of the domestic mosquito Culex pipiens, treated with the entomopathogenic fungus B. bassiana in suspension which corresponds to a dose of 107 spore/mL. In fact, after developing the individuals in the fungal solution, histological study was carried out on the following parts; cuticle, intestine, adipose tissue and hemolymph. The histological studies showed many alterations and malformation in the treated 4th instar larvae body and tissues. The infection affected the different parts of the body, especially the cuticle, the adipose cells, and midgut, in addition to the development and the colonization of B. bassiana inside tissues. The application of B. bassiana on the cuticle of the larvae was dependent on an apparent disturbance in the structure of the cuticle or the degeneration of its different parts. The fungus infection does not stop at the level of the body walls, so it even goes to adipose tissue, epidermal cells and intestine. Based on these results, B. bassiana could be suggested as suitable biocontrol agent against C. pipiens