Browsing by Author "Kebbouche-Gana, Salima"

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    Algerian yeast strains : isolation, identification and production of single cell protein from whey with strain candida kefyr
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine
    In this study, few samples than soft and baked cheese, yogurt, cow's milk, pasteurized milk and cheese whey were collected from dairy industries in the city of Boumerdes. The samples were cultured on yeast extract glucose chloramphenicol agar (YGCA) media. Twenty yeast strains were isolated from the culture. Ten of theses strains were identified by their morphological and physiological properties. Betagalactosidase activity in the yeast strains showed that a strain Candida kefyr designated as LP1 had highest enzyme activity (up to 5000 EU/ml). To investigate the effectiveness of bat ch submerged fermentation of the yeast C . kefyr in cheese whey, we found that this strain have highest level of single cell protein (SCP), production (up to 19 g/l dry mass cell). The isolated yeast strain was examined for his ability in reduction of the c hemical oxygen demand (COD). So, the results demonstrated a high level of reduction
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    Antagonistic activity of Bacillus sp. obtained from an Algerian oilfield and chemical biocide THPS against sulfate-reducing bacteria consortium inducing corrosion in the oil industry
    (2010) Gana, M.L.; Kebbouche-Gana, Salima; Touzi, A.; Zorgani, M.A.; Pauss, A.; Lounici, H.; Mameri, N.
    The present study enlightens the role of the antagonistic potential of nonpathogenic strain B21 againstsulfate-reducing bacteria (SRB) consortium. The inhibitor effects of strain B21 were compared with those of the chemical biocide tetrakishydroxymethylphosphonium sul- fate (THPS), generally used in the petroleum industry. The biological inhibitor exhibited much better and effective performance. Growth of SRB in coculture with bacteria strain B21 antagonist exhibited decline in SRB growth, reduction in production of sulfides, with consumption of sulfate. The observed effect seems more important in comparison with the effect caused by the tested biocide (THPS). Strain B21, a dominant facultative aerobic spe- cies, has salt growth requirement always above 5% (w/v) salts with optimal concentration of 10–25%. Phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain B21 is a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis DQ115802 (94.0% sequence similarity), Bacillus aiding- ensis DQ504377 (94.0%), and Bacillus salarius AY667494 (92.2%). Comparative analysis of partial 16S rRNA gene sequence data plus physiological, biochemical, and phe-notypic features of the novel isolate and related species of Bacillus indicated that strain B21 may represent a novel species within the genus Bacillus, named Bacillus sp. (EMBL, FR671419). The results of this study indicate the application potential of Bacillus strain B21 as a biocontrol agent to fight corrosion in the oil industry
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    ANTIBACTERIAL AND ANTIFUNGUL EFFECTS OF FLAVONOIDS ISOLATED FROM CRATEAGUS OXYACANTHA (L.) FRUITS IN THE NORTH ALGERIAN REGIONS (BOUMERDES AND BAINEM)
    (STEF92 TECHNOLOGY LTD, 2014) Behidj-Benyounes, Nassima; Dahmane, Thoraya; Behidj, Khadidja-Kenza; Chebouti, Nadjiba; Kebbouche-Gana, Salima
    The extracts of plants are currently the subject of many scientific researches aiming to exploit their properties antimicrobial appreciated so much in the therapeutic, nutritional, cosmetic, and pharmaceutical fields. In this work, we have study the antimicrobial activity of the extracts namely flavonoids resulting from the Crateagus oxyacatha fruits in two bioclimatic stages (Dellys and Bainem). The determination of the antimicrobial effects on stocks bacterial of Gram(-); Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae and Gram(+) (Bacillus subtilis and Staphylococcus aureus). We have also tested two yeasts; Candida albicans and Saccharomycec cereviciae. As regards the extract of C. oxyacantha of the area of Bainem, one announces that E. Coli is slightly sensitive to the extract of C. Oxyacantha. However S. aureus and B. subtilis have a strong sensitivity to this extract. Whereas the other bacteria are fairly sensitive. With regard to yeasts, one notices that S. cerevisiae is slightly resistant to the extract. This solution has an inhibiting effect on C. albicans. Concerning the extract of C. oxyacantha of the area of Dellys, one notes that E. Coli is slightly sensitive to the extract of C. Oxyacantha. However S. aureus and B. subtilis have a strong sensitivity to this extract. Whereas the other bacteria are fairly sensitive. With regard to yeasts, one notices that S. cerevisiae is slightly resistant to the extract. This extract has a strong inhibiting effect on C. albicans. Through this study, one notices clearly the sensitivity of bacteria Gram-plus compared to the bacteria Gram-for the two areas.
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    Bactéries du sel et biosurfactants : entre isolement, screening et production
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine
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    Biocorrosion of carbon steel by sulfate-reducing consortium obtained from an algerian oil field that utilize nitrate
    (2011) Gana, M.L.; Kebbouche-Gana, Salima; Lounici, H.; Mameri, N.
    Biooxidation of sulphide under denitrifying conditions is a key process in control of souring in oil reservoirs and in treatment of gas and liquids contaminated with sulphide and nitrate. The effect of nitrate injection on the microbial community has already been evaluated in offshore oil industry production, but has never been studied in onshore such as Algerian oilfield. In this work, the SRB consortiums isolated by inoculating saline Postgate's medium C with injected water obtained from the In Amenas oil field, situated in the South Eastern Algerian Sahara was tested in the presence of sulfate, when nitrate was dosed at 120 mg/l it was reduced by this consortium bacteria, with some ammonium production. Therefore, this mechanism could be important in oilfield systems where nitrate is applied to prevent sulfide generation by SRB which leads to reservoir souring. In static tests the influence of this SRB consortium bacterium on corrosion was assessed using carbon steel coupons, in the presence of sulfate and in the presence of sulfate with 120 mg/l nitrate. Furthermore, the occurrence of pitting corrosion was fairly low under this circumstance
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    Biodegradation of Petroleum hydrocarbons and Biosurfactant production by an extremely halophilic Archaea Halovivax sp. A21
    (Aljest, 2017) Khemili-Talbi, S.; Kebbouche-Gana, Salima; Akmoussi-Toumi, S.; Gana, M.L.; Lahiani,S.; Ferrioune ,I.; Angar,Y.
    Over the recent few years, biosurfactant has played an important role in the industrial application especially in oil recovery; even in high salinity conditions. The potential of biosurfactant production by the extreme halophilic archaeon Halovivax sp. A21 in the presence of petroleum hydrocarbons (2% v/v) as sole carbon source at high salinity (25% NaCl) has been investigated. The results show the ability of Halovivax sp. A21 to grow and reduce surface tension under an optimum range of pH (7-9), salinities (15-35% NaCl) and temperature (40-45°C) for an optimized volume of 100 ml of the medium for 1000 ml capacity Erlenmeyer flasks with an optimum agitation of 120 rpm. The rates of biosurfactant production on petroleum hydrocarbons were enhanced with increasing NaCl concentration in the medium with an optimum of 25%. Biosurfactant production by Halovivax sp. A21 showed high emulsifying activity (more than 80%) and decreased surface tension (24.5 mN/m). The stability of the produced biosurfactant was determined by different physico-chemical conditions like pH, temperature and salinity. Moreover, the partial purification of the derived biosurfactant by silica gel column chromatography and Thin-layer chromatography revealed that it belongs to the lipopeptide group. Although both catechol dioxygenases participated in the degradation of petroleum hydrocarbons, more induction of catechol 1,2 dioxygenase was observed than the catechol 2,3 dioxygenase which indicated the predominance of the ortho cleavage pathways in the petroleum hydrocarbons degradation by the halophilic strain Halovivax sp. A21. The results demonstrated that strain Halovivax sp. A21 was able to increase the bioavailability of insoluble hydrocarbons, thus facilitating their uptake and their biodegradation even at high salt concentration. Likewise, the search of novel biosurfactants in extremophiles, or the use of microorganisms that present excellent
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    Biosurfactant production from newly isolated Rhodotorula sp.YBR and its great potential in enhanced removal of hydrocarbons from contaminated soils
    (Springer, 2021) Derguine-Mecheri, Louiza; Kebbouche-Gana, Salima; Djenane, Djamel
    One of the very promising methods in the field of bioremediation of hydrocarbons is the application of biosurfactant- producing microorganisms based on the use of wastewater as renewable substrates of culture media, contributing to the reduction of costs. With this aim, the production, characterization and properties of the yeast strain YBR producing a biosurfactant newly isolated from an oilfield in Algeria, using wastewater from olive oil mills (OOMW) as a substrate for a low-cost and effective production, have been investigated. Screening of biosurfactant production was carried out with different tests, including emulsification index test (E24), drop collapse test, oil spreading technique and measurement of surface tension (ST). The isolated yeast strain was found to be a potent biosurfactant producer with E24 = 69% and a significant reduction in ST from 72 to 35 mN m-1. The study of the cultural, biochemical, physiological and genetic characteristics of the isolate allowed us to identify it as Rhodotorula sp. strain YBR. Fermentation was carried out in a 2.5 L Minifors Bioreactor using crude OOMW as culture medium, the E24 value reached 90% and a reduction of 72 to 35 mN m-1 in ST. A biosurfactant yield = 10.08 ± 0.38 g L-1 was recorded. The characterization by semi-purification and thin layer chromatography (TLC) of the crude extract of biosurfactant showed the presence of peptides, carbohydrates and lipids in its structure. The crude biosurfactant exhibited interesting properties such as: low critical micellar concentration (CMC), significant reduction in ST and strong emulsifying activity. In addition, it has shown stability over a wide range of pH (2-12), temperature (4-100 °C) and salinity (1-10%). More interestingly, the produced biosurfactant has proven to be of great potential application in the remobilization of hydrocarbons from polluted soil with a removal rate of greater than 95%
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    Biotechnology-2014 biodegradation performance of phenol by free and alginate entrapped cells of Haloarcula strain D21, an extremely halophilic bacterium isolated from a solar saltern (Ain Salah, Algeria)
    (2014) Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Khemili, Souad
    Immobilization is a general term that describes many different forms of cell attachment or entrapment. These different forms include encapsulation of cells in a polymer-gel and entrapment in a matrix. The Advantages of these techniques are to reduced possibility of inoculum contamination during storage, transport and application. So, beads are non toxic, biodegradable and non-polluting and can be produced in large quantities, stored for extended periods. Moreover, phenols and phenolic compounds are widely distributed as environmental pollutants due to their common presence in the effluents of many industrial processes, including oil refineries, ceramic plants, coal conversion process, phenolic resins, pharmaceutical and food Industries. The aim of this study was to compare the biodegradation performance of phenol by using free and encapsulated cells of Haloarcula strain D21 isolated from crude oil contaminated saline water collected at Ain Salah in Algeria. Batch experiments were carried out in order to obtain the maximum phenol degradation rates by analyzing the influence of the immobilization in calcium-alginate gel beads on biodegradation performance
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    Caldicoprobacter algeriensis sp. nov. a new thermophilic anaerobic, xylanolytic bacterium isolated from an algerian hot spring
    (2010) Bouanane-Darenfed, A.; Laure Fardeau, M.; Gregoire, P.; Manon, J.; Kebbouche-Gana, Salima; Benayad, T.; Hacene, H.; Luc Cayol, J.; Ollivier, B.
    A thermophilic anaerobic bacterium (strain TH7C1 T ) was isolated from the hydrothermal hot spring of Guelma in the northeast of Algeria. Strain TH7C1 T stained Gram-positive, was a non-motile rod appearing singly, in pairs, or as long chains (0.7–1 9 2–6 l m 2 ). Spores were never observed. It grew at temperatures between 55 and 75 C (optimum 65 C) and at pH between 6.2 and 8.3 (optimum 6.9). It did not require NaCl for growth, but tolerated it up to 5 g l - 1 . Strain TH7C1 T is an obligatory heterotroph fermenting sugars including glucose, galactose, lactose, raffinose, fructose, ribose, xylose, arabinose, maltose, mannitol, cellobiose, mannose, melibiose, sac- charose, but also xylan, and pyruvate. Fermentation of sugars only occurred in the presence of yeast extract (0.1%). The end-products from glucose fermentation were acetate, lactate, ethanol, CO 2 , and H 2 . Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The G ? C content of the genomic DNA was 44.7 mol% (HPLC techniques). Phy- logenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence indicated that strain TH7C1 T was affiliated to Firmicutes , order Clostridiales , family Caldi- coprobacteraceae , with Caldicoprobacter oshimai (98.5%) being its closest relative. Based on phenotypic, phyloge- netic, and genetic characteristics, strain TH7C1 T is pro- posed as a novel species of genus Caldicoprobacter , Caldicoprobacter algeriensis , sp. nov. (strain TH7C1 T = DSM 22661 T = JCM 16184 T )
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    Characterization of a purified thermostable xylanase from caldicoprobacter algeriensis sp. nov. strain TH7C1T
    (Elsevier, 2016) Bouanane-Darenfed, Amel; Boucherba, Nawel; Bouacem, Khelifa; Gagaoua, Mohammed; Joseph, Manon; Kebbouche-Gana, Salima; Nateche, Farida; Hacene, Hocine; Ollivier, Bernard; Cayol, Jean-Luc; Fardeau, Marie-Laure
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    Chemical composition, antimicrobial and insecticidal activities of Olea europaea L. ssp. sativa. collected from East of Algeria
    (Springer, 2025) Brahmi, Fairouz; Benlefki, Nawal; Koullal, Radwa; Kebbouche-Gana, Salima; Lenchi, Nesrine
    Olea europaea L., commonly known as the olive tree, is extensively utilized in Algeria for its medicinal properties. This plant is rich in alkaloids, phenolic acids, and flavonoids. The aim of this study was to evaluate the antimicrobial and insecticidal activities of methanol extracts and oils harvested from the aerial parts of Olea europaea L. ssp. sativa from two different regions (Bouira and Bordj Bouarreridj). Adult aphids (Aphis fabae) treated with crude methanol extracts exhibited high mortality rates, reaching up to 100%, while diluted extracts caused mortality rates between 80 and 100%. In terms of antimicrobial activity, all tested strains exhibited resistance (including E. coli, B. subtilis, S. aureus, K. pneumoniae, S. agalactiae, and C. albicans) for all methanol extracts and both oils. However, significant inhibition percentages were noted against the fungus Cladosporium sp. across the various tested extracts. These extracts have the potential to be used in the food, pharmaceutical and agricultural industries
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    Chitosan nanoparticles with controlled size and zeta potential
    (John Wiley and Sons Inc, 2023) Benamer-Oudih, Samah; Tahtat, Djamel; Nacer Khodja, Assia; Mahlous, Mohamed; Hammache, Yasmina; Guittoum, Abd-Errahim; Kebbouche-Gana, Salima
    Optimization of chitosan nanoparticles (ChNs) production process employing a 2(5–2) fractional factorial design was performed to analyze the influence of viscosity average molecular weight (40–120 kDa), the initial concentration of chitosan (2–5 g/L), the initial tripolyphosphate (TPP) concentration (0.8–1.2 g/L), the ratio chitosan/TPP (4/1–10/1) (V/V), and the stirring speed (300–700 rpm), on final nanoparticles size and zeta potential. The measured responses of average particle size and surface charge were determined on Zetasizer Nano ZS. ChNs were prepared using ionotropic cross-linking of chitosan and TPP and were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), and differential scanning calorimetry (DSC). The experiments showed that the size of synthesized nanoparticles depended on initial concentration and molecular weight of chitosan, TPP concentration and stirring speed within the chosen levels. However, the zeta potential was significantly influenced by chitosan molecular weight, chitosan concentration and stirring speed. The FTIR analysis confirmed the interaction between negative charge of TPP with positive charge of chitosan through the appearance of new peaks at 1222 and 895 cm−1 in produced ChNs. XRD and DSC analysis were used to evaluate the effect of crosslinking of chitosan on crystal structure of ChNs
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    Comparison of biological nitrate reduction effectiveness of two strains isolated from activated sludge
    (Chemical Publishing, 2016) Angar, Yassmina; Kebbouche-Gana, Salima; Djelali, Nacer-Eddine
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    Comparison of microbial community composition in injection and formation water from Algerian oilfields
    (2012) Lenchi, Nesrine; Inceoğlu, Özgül; Kebbouche-Gana, Salima; Gana, Mohamed Lamine; Llirós, Marc; Servais, Pierre; Garcia-Armisen, Tamara
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    A Comprehensive Study on Crude Methanolic Extract of Daphne gnidium L. as Effective Corrosion Inhibitors of Mild Steel Induced by SRB Consortium
    (Springer, 2019) Djouahra-Fahem, Djamila; Angar, Yassmina; Lamine Mohamed, Gana; Khoukhi, Faiza; Kebbouche-Gana, Salima
    The aim of the present work is the evaluation of effect of methanolic extract obtained from Daphne gnidium against biocorrosion caused by sulphate-reducing bacteria (SRB). Herein, the study of the influence of SRB consortium has been realized on the biological and electrochemical properties of the carbon steel API5LX60 immersed in water sample obtained from an Algerian oil field separator. The monitoring of the treatment effects on the SRB performance using kits test and weight loss methods showed a positive effect of the methanolic extract of D. gnidium as a corrosion inhibitor at a concentration of 0.8 g/L. In the other hand, the weight loss test has generated an efficiency rate of 95.99% at a concentration of 1.6 g/L. A linear polarization resistance approved these results, and they have given a yield of 91.14% with a polarization resistance value of 28.9 kΩ cm2 at a concentration of 0.25 g/L.