Browsing by Author "Lounici, H."

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Antagonistic activity of Bacillus sp. obtained from an Algerian oilfield and chemical biocide THPS against sulfate-reducing bacteria consortium inducing corrosion in the oil industry
(2010) Gana, M.L.; Kebbouche-Gana, Salima; Touzi, A.; Zorgani, M.A.; Pauss, A.; Lounici, H.; Mameri, N.
The present study enlightens the role of the antagonistic potential of nonpathogenic strain B21 againstsulfate-reducing bacteria (SRB) consortium. The inhibitor effects of strain B21 were compared with those of the chemical biocide tetrakishydroxymethylphosphonium sul- fate (THPS), generally used in the petroleum industry. The biological inhibitor exhibited much better and effective performance. Growth of SRB in coculture with bacteria strain B21 antagonist exhibited decline in SRB growth, reduction in production of sulfides, with consumption of sulfate. The observed effect seems more important in comparison with the effect caused by the tested biocide (THPS). Strain B21, a dominant facultative aerobic spe- cies, has salt growth requirement always above 5% (w/v) salts with optimal concentration of 10–25%. Phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain B21 is a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis DQ115802 (94.0% sequence similarity), Bacillus aiding- ensis DQ504377 (94.0%), and Bacillus salarius AY667494 (92.2%). Comparative analysis of partial 16S rRNA gene sequence data plus physiological, biochemical, and phe-notypic features of the novel isolate and related species of Bacillus indicated that strain B21 may represent a novel species within the genus Bacillus, named Bacillus sp. (EMBL, FR671419). The results of this study indicate the application potential of Bacillus strain B21 as a biocontrol agent to fight corrosion in the oil industry
Biocorrosion of carbon steel by sulfate-reducing consortium obtained from an algerian oil field that utilize nitrate
(2011) Gana, M.L.; Kebbouche-Gana, Salima; Lounici, H.; Mameri, N.
Biooxidation of sulphide under denitrifying conditions is a key process in control of souring in oil reservoirs and in treatment of gas and liquids contaminated with sulphide and nitrate. The effect of nitrate injection on the microbial community has already been evaluated in offshore oil industry production, but has never been studied in onshore such as Algerian oilfield. In this work, the SRB consortiums isolated by inoculating saline Postgate's medium C with injected water obtained from the In Amenas oil field, situated in the South Eastern Algerian Sahara was tested in the presence of sulfate, when nitrate was dosed at 120 mg/l it was reduced by this consortium bacteria, with some ammonium production. Therefore, this mechanism could be important in oilfield systems where nitrate is applied to prevent sulfide generation by SRB which leads to reservoir souring. In static tests the influence of this SRB consortium bacterium on corrosion was assessed using carbon steel coupons, in the presence of sulfate and in the presence of sulfate with 120 mg/l nitrate. Furthermore, the occurrence of pitting corrosion was fairly low under this circumstance
Biohydrogen production by dark and photo-fermentation processes
(IEEE, 2013) Akroum-Amrouche, Dahbia; Abdi, N.; Lounici, H.; Mameri, N.
Chlorophytum rhizosphere, a suitable environment for electroactive biofilm development
(Springer, 2020) Tou, Insaf; Azri, Mounia; Sadi, Meriem; Zitouni, D.; Merad3, A. S.; Laichouchi, A.; Drouiche, N.; Lounici, H.; Gana-Kebbouche, S.
The electroactivity of Chlorophytum rhizospheric soil using imposed potential chronoamperometry and a characteristic cyclic voltammetry was demonstrated in the present work. Five different polarizations were tested: − 0.3, − 0.155, 0, + 0.155, and + 0.3 V/SCE. The current density had stabilized around − 0.0068 mA/m2 and − 0.03 mA/m2 at − 0.155 and − 0.3 V/SCE, respectively. However, at 0, + 0.155, and + 0.3 V/SCE, a current density had reached respectively 1.46 A/m2, 1.48 A/m2, and 0.6 A/m2. The potential + 0.155 V/SCE seemed to better stimulate the electrogenic bacteria activity of the Chlorophytum rhizosphere. Different bacterial strains had formed electroactive biofilms in response to different electrode polarizations. The Chlorophytum rhizosphere electroactivity has depended on strict anaerobes as well as facultative anaerobic bacteria under anaerobic conditions. Furthermore, the Chlorophytum rhizosphere soil had closed almost equal proportions of Firmicutes and Proteobacteria whose electroactivity seemed to depend on the Proteobacetria more than the Firmicutes and had could be thereby a suitable environment for electroactive biofilm development