Browsing by Author "Mohammedi, Arezki"

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Antimicrobial potential of ziziphus and euphorbia honeys harvested in semi-arid region of Algeria and their possible use in soft medicine
(EBSCO, 2020) Haderbache, Latifa; Annou, Saada; Mohammedi, Arezki
Two different botanical origin honey types (Ziziphus lotus and Euphorbia bupleuroides) from semi-arid regions in Algeria consisting of twelve samples were tested for their antimicrobial efficiency. Global assessment of antimicrobial activity was made by wells method on integer samples and by turbidity test to locate fraction responsible of this activity. Honeys have been tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Fungal strain was resistant to all honeys at all concentrations, whereas E. coli and S. aureus were sensitive presenting minimum inhibition concentrations (MIC) between 10 and 50%. Euphorbia honeys appeared to be more active. The fractionation shows that volatile fraction can have great antimicrobial effect, followed by the acidic one. Correlations reveal good relation between inhibitory effect, free acidity and polyphenols. These facts show large possibilities for honey use in soft medicine against some bacterial infections. Copyright of Journal of Microbiology, Biotechnology & Food Sciences is the property of Slovak University of Agriculture in Nitra, Faculty of Biotechnology & Food Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract
Dietary use of Rosmarinus officinalis and Thymus vulgaris as anticoccidial alternatives in poultry
(Elsevier, 2021) Aitfella Lahlou, Radhia; Bounechada, Mustapha; Mohammedi, Arezki; Silva, Luís R.; Alves, Gilberto
Coccidiosis is a parasitic disease caused by protozoa belonging to the genus Eimeria and whose propagation has a tremendous economic impact on poultry production. The parasite develops in the intestinal tract and causes disturbances in homeostasis that may lead to the death of the animal. Anticoccidial drugs and vaccination are the main preventive measures used against this disease. However, the rapid spread of drug resistance and the emergence of environmental pollutants have led to search other alternatives that do not hinder the health and productivity of broilers. The use of medicinal plants or plant-derived products could be one of these approaches; in fact, plants are reservoirs of bioactive compounds that could be developed as new chemical entities. Two members of the Lamiaceae family, Rosmarinus officinalis and Thymus vulgaris included in the Algerian pharmacopoeia have been reviewed herein, as well as their anticoccidial activity. We discuss their potential uses as natural growth promoters, referring their phytochemical constituents and biological properties that could play a crucial role in improving the symptoms caused by coccidiosis in poultry
Differences in grooming behavior between susceptible and resistant honey bee colonies after 13 years of natural selection
(Springer Nature, 2020) Dadoun, Nedjma; Nait-Mouloud, Mohamed; Mohammedi, Arezki; Sadeddine Zennouche, Ourdia
The present study was conducted to quantify at the individual level the grooming behavior of bees from resistant colonies and susceptible colonies. Experienced and naive bees from resistant colonies were compared to experienced and naive bees from susceptible colonies at the age of 4, 7, 15 and 21 days. In a total of 480 assays, resistant bees successfully groomed off 10 times more mites placed than susceptible bees. Worker of different ages are involved but the lowest percentage of grooming was observed in 21-day-old bees. The experienced bees from resistant colonies bees that have evolved in a natural environment removed significantly more mites (69.2%) compared to naive bees (51.7%) who had no contact with other older bees.
Effect of heat stress on Apis mellifera intermissa and Apis mellifera sahariensis in laboratory controlled conditions: a physiological approach
(Taylor and Francis Ltd., 2024) Khedidji, Hassiba; Chellali, Meriem; Cherrered, Nawel; Ghaffar, Intissar; Ladjali-Mohammedi, Kafia; Mohammedi, Arezki
The effects of heat on honey bees have received attention in many subspecies. Nevertheless, some subspecies still remain unexplored. In this study, we investigate for the first time the effect of heat on two subspecies of honey bees endemic to Algeria, Apis mellifera intermissa which is adapted to the Mediterranean climate and Apis mellifera sahariensis, which is adapted to the hot climate of southwest Algeria. We tested the hypothesis that sahariensis is more tolerant to heat stress than intermissa. In two in-vitro tests, we reared workers of both subspecies at 4 temperatures (34 °C, 38 °C, 39 °C, and 40 °C). Then, we determined the hemolymph protein (HP) concentration, hypopharyngeal gland development (HPG), ovary development and survival. We found that the sahariensis had HPG more developed and less affected by heat than intermissa. Conversely, although intermissa had more (HP) at 34 °C, we found a significant increase in (HP) at 38 °C, 39 C° and 40 °C in intermissa than sahariensis. Although ovary development is greater in intermissa than sahariensis at 34 °C and greater at 34 °C than 38 °C, 39 °C and 40 °C, we did not record differences in ovary development between the subspecies at 38 °C, 39 °C, and 40 °C. Finally, in all conditions, sahariensis had a higher survival than intermissa.
Effects of pollen deprivation in groups of tellian (Apis mellifera intermissa) and saharan (Apis mellifera sahariensis) honey bees under controlled conditions
(MDPI, 2022) Khedidji, Hassiba; Abderrahmani, Khaled; Oulebsir-Mohandkaci, Hakima; Ladjali-Mohammedi, Kafia; Mohammedi, Arezki
Worldwide, honey bees are increasingly faced with periods of pollen scarcity, which can lead to nutritional deficiencies, especially of proteins and amino acids. These are essential for the proper functioning of the single organism and the colony. To understand how bees react to protein deficiency, under controlled conditions, we studied the effect of pollen deficiency on the main physiological parameters in two subspecies endemic of Algeria, Apis mellifera intermissa and Apismellifera sahariensis. Emerging workers of both subspecies were reared with two diets: one was pollen-fed, whereas the other pollen-deprived. Several physiological criteria were measured depending on the type of diet and subspecies: the survival of the bees, the amount of total protein in the hemolymph, hypopharyngeal glands development and the ovary development of workers. These last three parameters were assessed at three different ages (7, 14 and 21 days). At birth, sahariensis workers weighed more than intermissa. With the same protein diet, the average life expectancy of sahariensis was extended by 5.55 days compared to intermissa. Even if deprived of pollen, sahariensis lived longer than intermissa fed with pollen (p < 0.001). In the three age levels, the hypopharyngeal glands were more developed and less affected by pollen deficiency (p < 0.001) in sahariensis than in intermissa (p < 0.001). The total hemolymph protein was higher in intermissa than in sahariensis regardless of the diet, and was also higher in protein-fed than in deprived bees (p < 0.001). The ovaries developed more rapidly with a high proportion in intermissa than in sahariensis (p < 0.05) regardless of the diet, and was also higher in the bees fed with pollen than those deprived (p < 0.05). Pollen deficiency generates physiological alterations and modifications, the amplitude of which varied according to the subspecies of the bee studied
ENQUETE SUR LES PRODUITS PHARMACEUTIQUES UTILISES DANS LALUTTE CONTRE LE VARROA DANS LA REGION DE BOUMERDES
(2018-07-04) Rezal, Ahlem; Mohammedi, Arezki
La wilaya de Boumerdes est une région à haute potentialité apicole. Afin de mettre en évidence la situation de la lutte contre le parasite Varroa destructor et les produits pharmaceutiques utilisés, nous avons réalisé une enquête. Notre enquête a été menée dans la daïra de Khemis el khechna. Il s’en est avéré qu’il y’a peu de professionnels en apiculture. En outre près de 75℅ des apiculteurs ont moins de 55ans alors que 6 3℅ d’entre eux ayant un niveau moyen-secondaire et universitaire.Le domaine apicole reste dominé par les hommes qui représentent 94℅ de l’ensemble des apiculteurs interrogés. Quant au traitement, les produits pharmaceutiques les plus utilisés sont Apistan®, Apivar® et Bayvarol®. A la fin, ce travail doit être étendu sur l’ensemble des daïras de la wilaya de Boumerdes pour donner une idée plus précise.
Etude sur le comportement de consommation du miel en Algérie : attentes et préférences
(2015) Haderbache, Latifa; Mohammedi, Arezki
Cette étude tente de dresser un portrait, dans le contexte actuel de la filière apicole, de la perception du consommateur algérien du miel, de ses attentes et de ses préférences. Une enquête de consommation a été enclenchée sur une population de 800 répondants (questionnaire) de 20 wilayates. Les résultats montrent que l’algérien consomme environ 0,200 kg/an/h. Ces niveaux de consommations restent très faibles, donnant au miel une part insignifiante du régime alimentaire comparé à tous les bienfaits qu’il en attend. Le consommateur algérien considère le miel comme médicament, ses préférences se portent sur les miels foncés, liquides, emballés dans des bocaux en verre, mais il est dans la confusion totale quant au circuit commercial de celui-ci ; de ce fait, il se procure ses miels par les circuits informels et par des rapports de confiance ou se tourne vers les miels d’importation présents dans les commerces, Sans être sure de la qualité de ces produits
Isolation of entomopathogenic microorganisms from soil: study of the pathogenicity of an Aspergillus strain and its toxins against Galleria mellonella
(2020) Oulebsir-MohandKac, Hakima; Benzina-Tihar, Farida; Reghmit, Abdenacer; Lahian, Sadjia; Mohammedi, Arezki; Salhi, Khadidja; Salh, Widad
This study aims at finding an alternative to control bee enemies in order to protect this precious insect. Entomopathogenic microorganisms are isolated from the rhizosphere, and an Aspergillus strain was chosen to be tested against the wax moth Galleria mellonella and the poliniphagous beetle Trogoderma sp. The work of the first part was oriented towards the isolation of bacteria and fungi from soil and phenotypic identification of the fungal strain chosen. Then production and characterization of toxins produced by Aspergillus sp. by submerged fermentation using Maltose medium were done. The double extraction of the supernatant with dichloromethane and its separation by TLC allowed us to reveal the production of Ochratoxin A and Fumagillin. Biotests using different fungal suspensions (D1 = 5.2 * 10 3 sp/ml; D2 = 5.2 * 10 5 sp/ml, D3 = 5.2 * 10 7 sp/ml) and mycotoxins against the two pests allowed us to observe that the treatment did not affect Trogoderma beetle. By contrast, the wox moth was infected by the fungus. This result was confirmed by the qualitative and quantitative determination of the haemolymphatic metabolites which revealed a clear disturbance. The verification of the harmlessness of the fungus tested against *Corresponding author h.mohandkaci@univ-boumerdes.dz Apis mellifera showed that the highest dose 10 7 spores/ml is moderately toxic in honey bees. Finally, the application of minimum inhibitory doses of biocontrol agent Aspergillus will allow to avoiding possible mortalities to bees
Ziziphus Lotus and Euphorbia bupleuroides Algerian Honeys
(IDOSI Publications, 2013) Haderbache, Latifa; Bousdira, Mouna; Mohammedi, Arezki
Two Algerian honey types of semi-arid regions are characterized. Botanical origin is controlled by qualitative and quantitative melissopalynology. Based on dendrograms test, the 27 studied samples were classified into three homogeneous groups. The control analyzes showed that they are of good quality in agreement with the Codex Alimentarius. For discriminating between groups, physico-chemical parameters and phenolic and sugar composition were measured. But it is only the pH, free acidity, electrical conductivity and specific rotation that were powerful to differentiate unifloral Ziziphus Lotus group, Euphorbia and multiforal honeys containing Ziziphus . Ziziphus lotus honey presented specific characteristics, with high pH (4.96 ± 0.91), dextrogyrous specific rotation (-1.41 ± 7.55°) and high diastase activity (36.06 ± 10.06). While, Euphorbia honeys were within honey standards, with poor proline content (114.3 ± 30.3ppm) and low Diastase activity (14.6 ± 4.1Ush).The mineral fraction of ziziphus honey reveals the predominance of potassium, calcium and sodium with a constant presence of iron and magnesium. Finally, correlations analysis suggests the existence of a strong relationship between color and flavonoïd fraction, which is present in significant amounts (15-30 mg EQ/100g) compared to rates usually found in European monofloral honeys (1-6 mg EQ/100g), which is remarquable