Browsing by Author "Nabiev, Mohamed"

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Comparative analysis of the chemical constituents and in vitro antioxidant activities of different aqueous extracts of cistanche phelypaea (L.) Cout. from Algeria
(Elsevier, 2022) Rahim, Meriem Zohra; Prinsi, Bhakti; Espen, Luca; Benabdelkader, Tarek; Nabiev, Mohamed; Houali, Karim
Cistanches herba are well-known for their nutritional and therapeutic effects, but only few reports are available about the species Cistanche phelypaea (L.) Cout. and studies of aqueous extracts are scarce. This study aimed to elucidate the impact of aqueous extraction methods including decoction, infusion and cold maceration from C. phelypaea roots on phytochemical composition and in vitro antioxidant activities of the extracts. In order to accomplish this, determinations of total phenolic and flavonoid contents, liquid chromatography electrospray ionization mass spectrometry (LC/ESI-MS and LC/ESI-MS/MS) analyses and in vitro antioxidant activity studies were carried out. All extracts had significant levels of total phenolic content, but the decoction possessed the highest concentration and no significant difference was observed between infusion and cold maceration. Concerning flavonoids, decoction and infusion possessed the highest concentrations. Data obtained from LC/ESI-MS and LC/ESI-MS/MS analyses showed similar qualitative profiles for all aqueous extracts with 14 characteristic chromatographic peaks in negative mode and 6 in positive mode, which allowed the tentative identification of syringin, 14 phenylethanoid glycosides and 5 iridoids, including 5 pairs of isomers. However, significant variations were recorded for the relative abundance of some compounds depending on the method that was employed. Hot extraction procedures, decoction and/or infusion were more efficient in extracting acteoside, isoacteoside and two molecules corresponding to isomers of 2ʹ-acetylacteoside/tubuloside B, and it seems that a longer time of heating-extraction was required for an improved extraction of isoacteoside, and one of the two isomers of 2ʹ-acetylacteoside/tubuloside B, as decoction gave a significant higher amount of these compounds. However, 8-epiloganic acid and cistanoside F, with their respective isomers, and syringin were more extractable by the cold maceration process. All extracts had similar antioxidant properties in scavenging DPPH radical and total antioxidant activity assays, with the exception of the ferric-reducing power activity assay, in which cold maceration exhibited a significantly less potent activity. Aqueous extracts of C. phelypaea roots present an interesting antioxidant potential which is related to the synergistic effects of several antioxidant compounds
Development of a Scar Removal Ointment Containing Natural Active Ingredients
(BENTHAM SCIENCE, 2020) Benahmed Djilali, Adiba; Benseddik, Abdelouahab; Hacen, Katia; Boulahlib, Mohamed; Allaf, Karim; Nabiev, Mohamed; Issad, Mourad
Aims: The present study aimed to evaluate the healing effect of five new dermal ointments made with natural ingredients, derived from the Ficus microcarpa fruit, Pulicaria odora L. leaf powder, vaseline, lanolin, and sweet almond oil on thermal burns of New Zealand rabbits. Background: Many natural ingredients are used as alternatives in topical burn wound treatments. However, the effectiveness of an ointment is primarily assessed on the basis of the healing time. The latter depends on the synergetic effect resulting from the interaction of its different constituents. Hence there is the need to explore different formulations to find the most optimal one. Objectives: 1/ To evaluate some biochemical compositions, biological properties, and functional quality of F. microcarpa fruit and P. odora L. leaf powder., 2/ To identify the best combination between the F. microcarpa fruit, P. odora L. leaf powder, vaseline, lanolin, and sweet almond oil to obtain an ointment with high healing quality. Methods: Ten (10) New Zealand rabbits (5 males and 5 females) received two thermal burns caused by a hot cylindrical metal on their backs. Each rabbit was treated immediately thereafter with one of the elaborated ointments and with a commercial skin ointment Madecassol (reference). All the ointments were applied topically every other day until a complete epithelialization takes place. Evaluation of the healing process was based on the healing time and diameter narrowing calculated every seven days. Results: The obtained results show that all the developed ointments used in the experiments lead to total recovery with fur growth. However, the healing time varies from one formula to another. Conclusion: The study concludes that the combination of the natural active ingredients used in this study promotes burn wound healing in the rabbit’s model
Evaluation of physical‐chemical, pharmacodynamic and pharmacological attributes of hot air dried and swell dried jujube powders
(2016) Benahmed Djilali, Adiba; Nabiev, Mohamed; Gelicus, Antony; Benamara, Salem; Allaf, Karim
Liquid-liquid extraction and simultaneously spectrophotometric determination of Co (II) and W (VI) using crown ether (DB-18-C6) in aqueous media and in high speed steel
(Taylor and Francis Online, 2020) Saoud, Abdesselam; Hammadou née Mesdour, Souad; Arzu, Nabieva; Boudjema, Hamada; Abdeltif, Amrane; Nabiev, Mohamed
An analytical reagent ether crown DB-18-C-6 was extensively used forthe simultaneous and individual extraction and spectrophotometricdetermination of the tungsten and cobalt at microgram levels. Theligand reacts with two metals, and the ion-association systems wereextractable into chloroform giving a yellowish [(DB-18-C-6∙NH4)+2[WO(SCN)5]2]and[(DB-18-C-6∙NH4)+2[Co(SCN)4]2-]complexes.Theabsorption peaks of these complexes were found overlapping atλmax of 415 nm and 621.6 nm, respectively. Under optimum condi-tions, the [WO(SCN)4]2-] complexation and extraction are possible onlyin concentrated medium (2.5 M of HCl). While, the [Co(SCN)4]2-]wasextractable at a pH range of 4–10. The linear ranges were 29.45–-147.25μgmL−1for Co(II) and 1.8–183 μg/mL for W(VI) with molarabsorptivity,ε=1.7×104andε=1.6×104dm3mol−1cm−1,respec-tively. The accuracy and reproducibility of the proposed method forvarious amounts of cobalt and tungstenintheirbinarymixtureweretested, and the effects of diverse ions on their extraction were alsoinvestigated. The developed method was successfully applied for thesimultaneous determination of Co(II) and W(VI) in high-speed steel(HS2-9-1-8) containing 2% W, 9% Mo, 1% V, and 8% Co.
Microencapsulation of Thymus fontanesii extracts in pectin/casein : characterization, release behavior and storage stability
(Springer, 2021) Mebarki, Noudjoub; Ziane, Hanifa; Fazouane, Fethia; Nabiev, Mohamed
The present study aims to enhance the stability of Thymus fontanesii essential oil (TEO) and its main component thymol, and to prolong their release from encapsulation in pectin/casein matrix using complex coacervation and dried by freeze methods. Encapsulation efficiency, structural, physical and thermal properties of the microcapsules were determined. Storage stability of free and microencapsulated thyme extracts was evaluated and the controlled release kinetics of microcapsules in simulated gastrointestinal fluids were studied. Respectively, the successes of TEO and thymol encapsulation were confirmed by FTIR and X-ray diffraction analyses with encapsulation efficiency of 91.85 ± 1.35% and 93.66 ± 1.13%. The particle size ranged from 4.47 to 517.20 μm, presented a good thermal resistance and irregular shapes with rough surface. Prolonged release of the extracts from microcapsules was demonstrated with Higuchi behavior in gastric fluids while for simulated intestinal fluid, the non-Fickian transport was the main release mechanism. The microencapsulation of thyme extracts enhanced its thermal stability and proved that the thyme extracts were effectively well protected in microcapsules after a long time storage. The use of pectin/casein has shown good performance as a biodegradable matrix for protection and storage of thyme extracts, prolonging their storage stability and release. Therefore, these microcapsules can be useful ingredients for both food and pharmaceutical new products, to prolong bioavailability of bioactive compounds and to improve their thermal and storage stability