Browsing by Author "Rezzoug, Hamida"

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Caractérisation phytochimique et évaluation des propriétés pharmacologiques in vivo et in vitro des extraits de deux plantes médicinales Algérienne : formulation d’une forme
(Université M'Hamed Bougara Boumerdès : Faculté des Sciences, 2024) Rezzoug, Hamida; Bouchenak, Ouahiba(Directeur de thèse)
Cette étude vise à caractériser la composition chimique et d'évaluer la cytotoxicité et la bioactivité des huiles essentielles (HE) de Melissa officinalis (MO) et de Lavandula stoechas (LS) et l’effet synergique contre C. albicans afin de formuler des crèmes antifongiques. La composition chimique est analysée par GC-FID et GC/MS. Les interactions entre les deux huiles essentielles, ainsi que la cytotoxicité et les composés phénoliques, sont évalués. Les activités antioxydantes (par DPPH et ABTS•+), antibactériennes (sur 12 souches) et antifongiques (sur 9 souches) sont étudiées. Des formulations anti-candidose sont testées in vitro et in vivo contre Candida albicans. Les résultats montrent que les principaux composés de l'huile essentielle de MO sont le géranial (35,7 %) et le néral (24,1 %), tandis que le 1,8-cinéole (61,9 %) domine dans l'HE de LS. Ces deux huiles essentielles présentent une activité antioxydante plus importante contre les radicaux ABTS•+ que contre DPPH et une activité antibactérienne plus efficace contre les bactéries Gram+ que Gram-. De plus, une action antifongique substantielle contre tous les micro-organismes testés avec des valeurs de CMI allant de 0,125 à 0,0019 des HE de MO. L'huile essentielle combinée avec celle de L. stoechas montre également un effet anti- candidose significatif. Les crèmes formulées montrent un effet hautement significatif contre C. albicans in vitro et in vivo. Ces résultats suggèrent les bonnes qualités bioactives des crèmes topiques développées contre C. albicans, révélant leur potentiel pour être éventuellement appliquées comme agent antifongique en pharmacie industrielle
Commercial thyme essential oil as natural beverage preservative and molecular docking study on its mode of action against Saccharomyces cerevisiae
(Czech Academy of Agricultural Sciences, 2024) Lotmani, Zineb; Boukhatem, Mohamed Nadjib; Boudjema, Khaled; Rezzoug, Hamida; Benelmouffok, Amina Bouchra; Tomi, Pierre
The present investigation explored the possible use of Thymus vulgaris essential oil (TVEO) as a beverage antifungal preservative instead of chemical ones. The chemical profile of TVEO exposed carvacrol (60.47%) as the predominant compound. The antifungal properties of TVEO were assessed on various food spoilage yeast and mould species using two tests. TVEO showed a powerful antimicrobial effect against all the fungal strains at the three volumes of essential oil (EO) used (i.e. 10, 20, and 30 µL). The minimum inhibition concentration (MIC) of TVEO was also evaluated and ranged from 0.0625% to 0.015% (v/v). Furthermore, the potency of TVEO as a beverage antimicrobial preservative was tested at four distinct concentrations (0.6, 1.25, 4, and 6 μL·mL–1) against Saccharomyces cerevisiae alone and combined with medium heating (70 °C for 2 min) in a real food matrix (Orangina® drink) for eight storage days. TVEO exhibited a significantly higher preservative effect than chemical preservatives (sodium benzoate and potassium sorbate). Lastly, a molecular docking examined the mechanism of action of carvacrol against two crucial enzymes in S. cerevisiae viability [ERG2 (sterol C8-isomerase) and ERG3 (sterol C5-desaturase)] compared to a chemical preservative (potassium sorbate). The two ligands highly interacted with the two target enzymes. However, carvacrol achieved a better score than potassium sorbate against ERG2 and ERG3, with binding energy of –10.19 kcal·mol–1 and –11.73 kcal·mol–1, respectively. Our results open up the perspective of using TVEO as a natural food preservative.
A novel anti-candidiasis cream formulation based on Melissa officinalis and Lavandula stoechas essential oils synergism
(Taylor & Francis, 2024) Rezzoug, Hamida; Bouchenak, Ouahiba; Boudjema, Khaled; Benabdelkader, Tarek; Felix, Tomi; Pierre, Tomi; Djilani, Selma
Candida albicans is the yeast strain that causes candidiasis, which can vary from minor skin and soft tissue infections to potentially fatal infections like candidemia, which can get worse due to drug resistance. Natural substances could be used as antifungal agents’ alternatives, especially against C. albicans. Thereby, the main objective of this research was to define the chemical content and evaluate the bioactivity of Melissa officinalis and Lavandula stoechas essential oils (EOs). Additionally, the synergistic effect of both oils was carried out against C. albicans, in order to formulate EOs combination-based creams. Their chemical compositions were analyzed using the combination of gas chromatography-flame ionization detector (GC-FID) and GC-mass spectrometry (GC-MS). The interaction with both EOs was also evaluated and analyzed by GC-FID and GC-MS, and the development of anti-candidiasis formulations was tested in vitro. Our results showed that the main compounds of M. officinalis EO (MOEO) were geranial (35.7%) and neral (24.1%), whereas, 1.8-cineole (61.9%) was dominant in Lavandula stoechas essential oil (LSEO) and the main compounds of the combination between both EOs were 1,8-cineole and geranial. Moreover, the MOEO exhibited substantial antifungal action against all tested microorganisms with MIC values ranging from 0.125 to 0.0019. This EO also showed synergistic effect when combined with that of L. stoechas. These findings, suggest the good bioactive qualities of the topical creams developed against C. albicans, revealing their potential to be possibly applied as an antifungal agent in industrial pharmaceutics.