Publications Scientifiques
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Item Chemical composition and bioactivity of essential oil against the green peach aphid (Myzus persicae)(Springer, 2022) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Chahbar, Nora; Acheuk, Fatma; Mekhadmi, Nour El Houda; Benyahia, Ibtissem; Fethallah, Rabab; Hammi, Hamida; Berrekbia, Mohammed; Badjadi, ZeinebThe present experiment was conducted to elucidate the chemical composition of essential oil (EO) of a native spontaneous plant, Cotula cinerea Del., in southeastern Algeria and to evaluate its insecticidal effect on the Green Peach Aphid (GPA) Myzus persicae Sulzer. GC–MS and GC-FID analyses showed that this plant was characterized by 35 components, representing eight major constituents, i.e., santolina triene (11.55%), sabinene (5.86%), cineol (6.21%), α-thujone (22.91%), (R)-camphor (7.50%), cis-sabinyl acetate (6.41%), carveol (7.68%), and ipsenol (4.60%). The considered essential oil was characterized by a clear predominance of hydrocarbon compounds chiefly monoterpenes (35.63%), oxygenated monoterpenes (64%), and sesquiterpenes (0.35%). Insecticidal assays showed that the EO was toxic against M. persicae adults recording 75.95% of mortality in the 3rd dose with a lethal concentration (LC50) equal to 302.4 µl where the lethal time (LT50) was 91.6 h. Also, it has a significant repellency showing a high rate in the 2nd dose (64.6%) with a highly significant difference (p = 0.000). As well as its inhibitory effect on the egg-laying where it has greatly reduced the reproduction potential of M. persicae adults. Obtained data support the possibility to develop C. cinerea oil, especially component specification, as a new natural bio-insecticideItem Efficient and cost-effective production of Bacillus thuringiensis subsp. aizawai spores and delta-endotoxins using agricultural raw materials and agro-industrial wastes under submerged fermentation(Elsevier, 2022) Gounina-Allouane, Rabia; Acheuk, Fatma; Sahir-Halouane, FatmaIn this study, cost-effective liquid culture media based on agricultural raw materials and agro-industrial wastes were developed for spores and delta-endotoxins production by a strain of B. thuringiensis subsp. aizawai used as a larvicide in wax moths control. The raw materials used include edible leguminous seeds, corn, wheat bran, dry palm date pulps, and corn forages. The agro-industrial wastes were dry palm dates pits and olive mill solid wastes. The protein and carbohydrate contents of the developed media were measured. Sporulation on the different developed media was monitored and delta-endotoxins produced were quantified. Mono-component media containing chickpea, red lentils, and wheat bran produced a spore count compared to the reference medium NYSM. The results showed that the dry palm date's pulp extract was the most efficient medium for spore production (1.43 × 1010 spores ml−1), and the chickpea medium was the most efficient for delta-endotoxins productionItem Inhibitory effect of Trichoderma harzianum on mycelial growth of Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani(Springer Netherlands, 2018) Lakhdari, Wassima; Dehliz, Abderrahmene; Mlik, Randa; Hammi, Hamida; Benlamoudi, Wiam; Acheuk, Fatma; Doumandji-Mitiche, Bahia B.Tomato is one of the most important fruit vegetables for human consumption in Algeria. In recent years, the cultivation of this plant is experiencing a very rapid development. However, this speculation is confronted with several problems including plant health. The purpose of this study is to find biological ways to introduce them into an integrated control program against tomato pests and have an organic agriculture that can increase the production of this culture. Direct confrontation test of an indigenous strain of Trichoderma harzianum with two phytopathogenic fungi that are isolated from tomato leaves (Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani) was carried at 26 °C on an artificial culture medium (PDA). It could be demonstrated that T. harzianum inhibited mycelia growth of the two fungi with more than 40% and only after 4 days of incubation. Statistical analysis confirms that T. harzianum had a significant effect of antagonism against the tested pathogens mentioned before with P value 0.02 and 0.04, respectively. Beyond this period, and at the end of the sixth day, T. harzianum occupied the colonies of fungus and began to sporulate marking a very important myco-parasitic power. Indeed, a percent inhibition greater than 50% was recorded into the both fungiItem The aggregation pheromone phenylacetonitrile : joint action with the entomopathogenic fungus Metarhizium anisopliae var. acridum and physiological and transcriptomic effects on Schistocerca gregaria nymphs(Elsevier, 2020) Abdellaoui, Khemais; Miladi, Meriam; Mkhinini, Marouane; Boughattas, Iteb; Ben Hamouda, Amel; Hajji-Hedfi, Lobna; Tlili, Haithem; Acheuk, FatmaThe combined use of entomopathogenic fungi and sublethal rate of chemical insecticides or other biological control agents have been proposed as an environmentally and sustainable strategy in the control of locust pests. In this paper, the quarter and the half of the recommended dose of Metarhizium anisopliae var. acridum (¼ and ½ Ma) and the aggregation pheromone (Phenylacetonitrile: PAN) were applied simultaneously and sequentially to Schistocerca gregaria fifth-instar nymphs. In addition, the physiological effects of PAN on locusts were assessed at the behavior, immune response, and biochemical level by evaluating for glutathione-S-transferase (GST), acetylcholinesterase inhibition (AChE), and malondialdehyde accumulation (MDA). Results showed that simultaneous application of PAN and the entomopathogenic fungus exhibited additive interaction. Synergistic interaction was also demonstrated when nymphs were exposed to PAN first, then treated with M. anisopliae var. acridum. Behavioral bioassay revealed that fifth-instar nymphs avoided the PAN odour and tended to remain away from the stimulus cup. In the choice assay, the pheromone significantly repelled the locusts at 2, 4, and 6 h of exposure which selected the PAN-free arena chamber. Moreover, treated nymphs become hyperactive and disoriented as evidenced by the cumulative distance travelled and the trajectory of locusts during the experiment. Immunological studies showed that PAN significantly decreased the differential haemocyte counts (prohemocytes and plasmatocytes) with a dose-response relationship. Data of biochemical analyzes showed that the PAN exposure reduced the activity of acetylcholinesterase and induced significantly the glutathione S-transferases and MDA concentration in the desert locust fifth-instar nymphs. Moreover, transcriptomic responses to the PAN exposure were evaluated using gene expression levels of CYP540 and GST. The transcript levels showed an up-regulation in GST expression level particularly in nymphs exposed for 4 and 6 h. A significant increase in CYP450 transcript level was also observed after 2 h of exposure, which decreased significantly after 4 and 6 hItem Study of melliferous plants visited by the worker honeybee, Apis mellifera intermissa in Boumerdes, Algeria(Faculty of Agriculture, University of East Sarajevo, 2020) Belaid, Messaouda; Mohamedi, Arezki; Chahbar Adidou, Nora; Acheuk, Fatma; Abbad-Bennour, Malika; Ighil Ameur, AmelThis work allows to establish a list of melliferous plants visited by the worker bee, Apis mellifera intermissa in Boumerdes through melissopalynological analysis. Eight samples of honey were collected in summer from different regions of Boumerdes province in Algeria: Mizrana (H1), Tizéruine (H2), Tagdempt (H3), Sherraba (H4), Baghlia (H5), Boudouaou (H6), Sidi Daoud (H7) and Si Mustapha (H8). For the analysis, we adopted the classical method of Louveaux et al., (1970). The results obtained showed that the melissopalynological analysis indicated the pollen grains of Eucalyptus were numerous as more than 45% of the pollen in six (6) of the eight samples of Boumerdes (H1, H2, H3, H4, H7 and H8). The pollen of Hedysarum coronarium was dominant in the sample H5 (67.60%). In seven samples honey recognized multifloral by beekeepers, Eucalyptus and Citrus taxa were respectively super represented and underrepresented in the honey H7 (87.46%) and H6 (19.80%). Thus, the number of taxa listed in the honeys studied varies from 14 to 19. The total number of pollen grains counted for the 8 samples varied from 646 to 3580