Publications Scientifiques

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Author: search.filters.author.Acheuk, FatmaSubject: search.filters.subject.Bio-insecticide

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    Chemical composition and bioactivity of essential oil against the green peach aphid (Myzus persicae)
    (Springer, 2022) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Chahbar, Nora; Acheuk, Fatma; Mekhadmi, Nour El Houda; Benyahia, Ibtissem; Fethallah, Rabab; Hammi, Hamida; Berrekbia, Mohammed; Badjadi, Zeineb
    The present experiment was conducted to elucidate the chemical composition of essential oil (EO) of a native spontaneous plant, Cotula cinerea Del., in southeastern Algeria and to evaluate its insecticidal effect on the Green Peach Aphid (GPA) Myzus persicae Sulzer. GC–MS and GC-FID analyses showed that this plant was characterized by 35 components, representing eight major constituents, i.e., santolina triene (11.55%), sabinene (5.86%), cineol (6.21%), α-thujone (22.91%), (R)-camphor (7.50%), cis-sabinyl acetate (6.41%), carveol (7.68%), and ipsenol (4.60%). The considered essential oil was characterized by a clear predominance of hydrocarbon compounds chiefly monoterpenes (35.63%), oxygenated monoterpenes (64%), and sesquiterpenes (0.35%). Insecticidal assays showed that the EO was toxic against M. persicae adults recording 75.95% of mortality in the 3rd dose with a lethal concentration (LC50) equal to 302.4 µl where the lethal time (LT50) was 91.6 h. Also, it has a significant repellency showing a high rate in the 2nd dose (64.6%) with a highly significant difference (p = 0.000). As well as its inhibitory effect on the egg-laying where it has greatly reduced the reproduction potential of M. persicae adults. Obtained data support the possibility to develop C. cinerea oil, especially component specification, as a new natural bio-insecticide
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    Euphorbia guyoniana ethanolic extract efficiency against tomato leaf miner in southeastern Algeria
    (2020) Lakhdari, Wassima; Dehliz, Abderrahmène; Mlik, Randa; Benlamoudi, Wiam; Hammi, Hamida; Fathallah, Rabab; Benyahia, Ibtissam; Mekhadmi, Nour Elhouda; Acheuk, Fatma; Ouargli, Djamel
    The abusive use of insecticide on tomato crop may cause several impacts to environment and human aswell as they can provoke resistance to plants. For that reason, a biological alternative was tested by using the ethanolic extract ofEuphorbia guyoniana, spontaneous plant collected from arid regions in Southeastern Algeria, against eggs and larvae of tomato leaf minerTuta absoluta Meyrick (Lepidoptera: Gelechiidae). Materials and Methods: The 120 tomato leaf miner larvae T. absoluta of the L2-L3stages and eggs were tested into contact with E. guyoniana ethanolic extract within 3 doses chosen after several preliminary tests:20, 30 and 40 μg. Results: Experimentation showed the larvicidal effect of this plant varied with doses and time. The greatest mortalitywas obtained by the D3 (40 μg) with 50.83±19.54 and 95.42±03.15% noted after 2 and 96 h, respectively. The DL50 recorded was between22.69 and 19.49 μg/larva, involving 50% of mortality. The treatment of eggs showed mortality rates of 15±05, 35±05 (D 30 μg) and95±05% (D 40 μg) at the end of the test. Conclusion: The ethanolic extract of E. guyoniana revealed that it could constitute a good meansof managing T. absoluta that might be introduced in sustainable organic agriculture
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    Potential of Zygophyllum album L. to control Tuta absoluta in Southeastern Algeria
    (Springer, 2020) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Hammi, Hamida; Guezoul, Omar; Acheuk, Fatma; Benlamoudi, Wiam; Chergui, Salima; Guermit, Keltoum; Matallah, Salim; Berrekbia, Mohammed; Gheriani, Sofiane
    On the valorization of spontaneous plants to contribute to organic agriculture, a biological study was undertaken by using the aqueous extract of Zygophyllum album (family of Zygophyllaceae), collected from Southeastern Algeria. Three levels of treatment (100%, 50%, and 25%) diluted from the extracted solution of this plant were tested on larvae (IN = 30) and adults (IN = 30) of tomato borer Tuta absoluta. This work reveals larval mortality rates of 7.5 ± 0.58% after 24 h, 12.50 ± 2.38% after 48 h, 30 ± 4.08% after 72 h, 40 ± 4.08% after 96 h, and 42.5 ± 6.45% after 120 h, although, with 50% of the crude extract, values of 20 ± 5.77%, 40 ± 4.08%, 50 ± 4.08%, 65 ± 5.77%, and 70 ± 4.58% were recorded at the same test durations, respectively. When a 25% dose of this solution was used, a mortality rate of 35 ± 8.16% was noted after 24 h and exceeds double (77.5 ± 8.66%) in 120 h. These data show that the toxicity of Z. album aqueous extract against T. absoluta increases with time and from the highest dose to the smallest one. Females of T. absoluta lay only 10 ± 3.54 to 19 ± 3.61 eggs/leaflet on treated tomato leaves, but a double amount was recorded on the control (37.25 ± 13.15 eggs/leaflet). This observation shows a repulsive effect of Z. album against T. absoluta females