Publications Scientifiques
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Item Anticancer and antiproliferative activities of Algerian Origanum majorana L.’s essential oil on PC-3 and SKBR3 cells(Taylor and francis, 2024) Hafid, Nourehouda; Bouchenak, Ouahiba; Serttas, Riza; Bouhenna, Mustapha Mounir; Khiari, Ouiza; Oussaid, Sounia; Suat, ErdoganCancer is a prominent cause of death globally, with breast cancer and prostate cancer being among the most devastating types. Therefore, the available anticancer treatments have some drawbacks, like higher toxicity and limited bioavailability. Thus, this study aimed to investigate for the first time the anticancer activity of Algerian Origanum majorana L.’s essential oil (OMEO). This research assessed the chemical profile of Algerian OMEO by gas chromatography coupled with mass spectrometry (GC-MS). The analysis revealed 29 compounds, which represent 98.08% of total volatile oil. The major compounds identified in OMEO were terpinen-4-ol (21.37%), γ-terpinene (15.78%), α-terpinene (10.43%), and trans-sabinene hydrate (9.27%). Additionally, MTT (3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide) was also used to test the cytotoxicity on prostate cancer (PC-3), breast cancer (SKBR3), and normal retinal pigment epithelium (ARPE-19) cell lines. The results showed a selective cytotoxicity effect by decreasing cell viability of PC-3 cancer cells with half inhibitory concentration (IC50) of 608.57 μg/mL and 672.5 μg/mL after 48h and 72h, respectively. Regarding SKBR3 cancer cells, the IC50 was 991.5 μg/mL. OMEO exhibited no significant cytotoxicity against normal (ARPE-19) cells. Furthermore, we conducted a cell apoptosis assay using Hochest 33342 dye to explore the potential mechanism pathway of OMEO. The findings verified that OMEO could trigger apoptosis in PC-3 and SBKR3 cancer cells. The ability of OMEO to inhibit cell migration assessed via wound healing assay revealed a significant decrease in cell migration. Our results imply that OMEO decreases cell viability by inducing cell apoptosis. Moreover, the oil suppresses cell migration in prostate cancer and breast cancer cells.Item A novel anti-candidiasis cream formulation based on Melissa officinalis and Lavandula stoechas essential oils synergism(Taylor & Francis, 2024) Rezzoug, Hamida; Bouchenak, Ouahiba; Boudjema, Khaled; Benabdelkader, Tarek; Felix, Tomi; Pierre, Tomi; Djilani, SelmaCandida albicans is the yeast strain that causes candidiasis, which can vary from minor skin and soft tissue infections to potentially fatal infections like candidemia, which can get worse due to drug resistance. Natural substances could be used as antifungal agents’ alternatives, especially against C. albicans. Thereby, the main objective of this research was to define the chemical content and evaluate the bioactivity of Melissa officinalis and Lavandula stoechas essential oils (EOs). Additionally, the synergistic effect of both oils was carried out against C. albicans, in order to formulate EOs combination-based creams. Their chemical compositions were analyzed using the combination of gas chromatography-flame ionization detector (GC-FID) and GC-mass spectrometry (GC-MS). The interaction with both EOs was also evaluated and analyzed by GC-FID and GC-MS, and the development of anti-candidiasis formulations was tested in vitro. Our results showed that the main compounds of M. officinalis EO (MOEO) were geranial (35.7%) and neral (24.1%), whereas, 1.8-cineole (61.9%) was dominant in Lavandula stoechas essential oil (LSEO) and the main compounds of the combination between both EOs were 1,8-cineole and geranial. Moreover, the MOEO exhibited substantial antifungal action against all tested microorganisms with MIC values ranging from 0.125 to 0.0019. This EO also showed synergistic effect when combined with that of L. stoechas. These findings, suggest the good bioactive qualities of the topical creams developed against C. albicans, revealing their potential to be possibly applied as an antifungal agent in industrial pharmaceutics.Item Students’ perceptions regarding the abrupt transition to online learning during the COVID-19 Pandemic: Case of Faculty of Chemistry and Hydrocarbons at the University of Boumerdes—Algeria(American Chemical Society, 2020) Blizak, Meriem Djanette; Blizak, Salah; Bouchenak, Ouahiba; Yahiaoui, KarimaIn recent months, universities around the world have been forced to cancel courses and close their doors due to the growing coronavirus epidemic. Following the government’s precautions, Algerian students were bound to quarantine regulations for their safety. Thus, they unexpectedly switched to online learning instead of face-to-face learning. Our objective of this exploratory study is to investigate the perceptions of Algerian university students regarding the abrupt transition to online learning amid the COVID-19 pandemic. To achieve this goal, an online survey with closed and open-ended questions was conducted among 380 students from the faculty of chemistry and hydrocarbons (FCH) at the University of Boumerdes—Algeria. The results showed that students have a negative perception of online learning. They are reluctant about this new digital pedagogy and prefer the traditional way of teaching to online teaching during the coronavirus pandemicItem Effect of virgin olive and Pistacia Lentiscus oils fortified with tomato lycopene on biochemical parameters in Wistar rats(NAJFNR, 2021) Aidoud, Aziouz; Elahcene, Omar; Abdellaou, Zakia; Yahiaoui, Karima; Bouchenak, OuahibaBackground: Pistacia lentiscus oil (PLO) and virgin olive oil (VOO) contain a large variety of phytochemicals providing beneficial effects. Lycopene is the main carotenoid with antioxidant properties. The consumption of lycopene containing foods may fight against cardiovascular diseases. Aims: The present study aims to evaluate the effects of fortified oils (VOO and PLO) with lycopene on some biochemical parameters in Wistar rats. Material and Methods: The experimentation included 50 male Wistar rats from the Algerian Pasteur Institute for the duration of 9 weeks of treatment. Rats were divided into five experimental groups (n=10) and fed a different experimental diet each for 9 weeks: control group (C), Pistacia lentiscus oil group (PLO), lycopene- enriched Pistacia lentiscus oil group (PLO-Lyc), virgin olive oil group (VOO) and lycopene-enriched virgin olive oil (VOO-Lyc). Total Cholesterol (TC) concentration was determined by the enzymatic method CHOD-PAP, High-density lipoprotein-cholesterol (HDL-C) with Biotrol diagnostic, the levels of low-density lipoprotein-cholesterol (LDL-C) were calculated using the Friedewald formula (LDL-C=TC−HDL-C−TGs/5). Triglycerides (TG) were determined by the enzymatic method PAP-1000 and Serum phospholipids (PL) were determined by an enzymatic colorimetric method. The plasma atherogenic index (PAI) was calculated as (TC/HDL-C). Results: Results showed that ingestion of PLO and VOO diminished TC, LDL-C, TG, and PL levels, whereas the HDL-C levels raised in all the groups assayed. Moreover, the lowest level of plasma atherogenic index (PAI) was shown in the VOO- Lyc group after 3, 6, and 9 weeks of treatment. Conclusions: The enrichment of PLO and VOO with lycopene improved the beneficial effects derived from the consumption of both oils on serum biochemical parameters. These findings suggest that lycopene enriched PLO and VOO may be used as a natural product to defend against some cardiovascular diseases (CVD) as hyperlipidemic and hypercholesterolemic acquired disorders.Item Évolution de la fraction lipidique et protéique au cours delamaturation de la datte Deglet-Nour(2021) Yahiaoui, Karima; Bouchenak, Ouahiba; Arab, Karim; Benchabane, AhmedDeglet Nour dates at different stages of ripening were analyzed for their main chemical composition in lipids, fatty acids, proteins and amino acids. The following values (based on dry weight) were obtained for the five stages of ripening, respectively for lipids: from 1.25% Ms at the Hababouk stage to 6.33% Ms at the Kimri stage, 3.65% Ms at the Khâlal stage, 2.56% Ms at the Routab stage and 1.97% Ms at the Tamar stage. The fatty acids revealed are mainly myristic and palmitic acids. Protein analysis indicates values of 2.63% Ms at the Kimri stage and 1.25% Ms at the Tamar stage. Among the amino acids identified, only five were found to be present in significant amounts (glycine, -amino butyric acid, arginine, valine and lysine). From these results, it is difficult to overlook the importance of the lipid and protein fraction of the date in the formation of specific pigments and flavoursItem Evaluation de la fraction glucidique au cours de la maturation de la datte Deglet-nour(2020) Yahiaoui, Karima; Bouchenak, Ouahiba; Arab, Karim; Benchabane, AhmedLa concentration des glucides diffère au cours de la maturation de la datte Deglet Nour . Les teneurs en sucres totaux et réducteurs augmentent parallèlement avec la progression de la maturation du fruit jusqu'au stade Khâlal où les teneurs en saccharose atteignent leur maximum. La diminution de la teneur en saccharose et l'accroissement de la teneur en sucres réducteurs sont synchronisés avec l'activité de l'invertase, qui a été décelée sous la forme soluble et insoluble. L’activité optimale de l’invertase se situe à un pH de 4,5 et à une température de 30°C. Toutefois, les propriétés des fractions invertasiques soluble et insoluble de la datte Deglet-Nour sont approximativement identiquesItem Criblage phytochimique et évaluation du pouvoir antioxydant des feuilles de myrtus communis l. et rhamnus alaternus L.(Univérsité Saad Dahleb Blida 1, 2020) Bouchenak, Ouahiba; Yahiaoui, Karima; Benhabyles, Narimen; Laoufi, Razika; Toubal, Souheila; El Haddad, Djillali; Oussaid, Sounia; Blizak, Meriem Djanette; Arab, KarimDescription du sujet: les travaux d'investigation actuels sont axés sur la recherche de nouvelles molécules antioxydantes d'origine naturelle et proposition d'alternatives thérapeutiques.Objectifs: l'objectif de cette étude est de mettre en relation l'utilisation traditionnelle de Myrtus communis L. et Rhamnus alaternus L. par la population de Boumerdes et leur activité antioxydante.Méthodes: la mise en évidence du pouvoir antioxydant des deux plantes, consiste à un criblage phytochimique permettant d’avoir une idée sur la présence ou l'absence de certains métabolites primaires et secondaires. L'activité antioxydante est évaluée par la méthode de réduction du fer sur les trois extraits acétonique, méthanolique et éthanolique.Résultats: le screening phytochimique révèle une richesse des deux plantes en polyphénols, glucosides, saponosides, sucres réducteur et en flavonoïdes avec une absence des tanins catéchiques, d'anthocyanes, des caroténoïdes, des irridoides, des protéines, des stérols, des polyterpènes, d'amidon et des lipides. Le meilleur rendement est obtenu avec l'acétone à 48,5% pourMyrtus communis et Rhamnus alaternusà 47%. L'activité antioxydante est maximale pour Myrtus communisà la concentration de 1mg/ml à une densité optique pour les trois extraits : éthanolique (3,84), acétonique (3,46) et méthanolique (3,22) par rapport à l'acide ascorbique. Conclusion: ces résultats apportent un appui scientifique quant à l'utilisation traditionnelle de ces plantes, ouvrant ainsi des perspectives pour leur intégration dans les industries pharmaceutiques et alimentaires.Item L’importance des extraits d’Urtica dioica L. dans la lutte contre Culex pipiens (Linné, 1758)(2019) S. Toubal, Souheyla; Elhaddad, Djillali; Yahiaoui, Karima; Sadaoui, Nesrine; Arab, Karim; Bouchenak, OuahibaEn terme d'importance épidémiologique mondiale pour l'homme, les moustiques sont considérés comme le premier groupe de vecteurs. İls sont responsable de la nuisance (piqures douloureuses et gênantes) et de la transmission des maladies parasitaires comme le paludisme, le chikungunya, la fièvre jaune. La lutte contre les culicidés, comprend plusieurs méthodes, mais la lutte biologique reste la plus sure et la plus sélective, car elle dépend des ennemis naturels sans provoquer des dégâts sur l'environnement. Dans le cadre de ce travail une extraction, une analyse spectrale par infrarouge (IR) ainsi que l’évaluation de l’effet de l’extrait aqueux extraits et des alcaloïdes de la Grande ortie contre les moustiques Culex pipiens ont été effectué. L’analyse spectrale par infrarouge montre l’existence d’une diversité de groupement fonctionnel caractéristiques des alcaloïdes tel que le groupement alcyn, nitrile, ester et amide. Les résultats du test insecticide montrent également que l’extrait aqueux et celui des alcaloïdes d’Urtica dioica L., présentent des propriétés insecticides importantes dont les valeurs de la DL 50 pour les larves L4 sont de 4, 48 mg/ml pour l’extrait aqueux, et de 6, 91 mg/ml pour les alcaloïdesItem MALDI-TOF MS detection of endophytic bacteria associated with great nettle (Urtica dioica L.), grown in Algeria(2018) Toubal, Souheyla; Bouchenak, Ouahiba; Elhaddad, Djillali; Yahiaoui, Karima; Boumaza, SarahAny plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. The purpose of this work is to isolate and to identify the different bacteria from the internal tissues of Urtica dioica L. and to study their diversity. This last is based on the different parts of the plant (stems, leaves and roots) and the harvest regions (Dellys and Tlamcen). The identification of bacteria is done by biochemical tests and confirmed by MALDI-TOF MS. Seven genus and eleven species were isolated from the Great Nettle. They belong to the genera Bacillus, Escherichia, Pantoea, Enterobacter, Staphylococcus, Enterococcus and Paenibacillus. The majority of these bacteria were isolated from Tlemcen which makes this region the richest in endophytic bacteria compared to that harvested from Dellys. The results show also that the leaves are the most diversified in endophytic bacteria. Bacillus pumilus-ME is the common species of the three parts of the plant harvested in both regions. From this work, it emerges that the Great Nettle can be settled by various endophytic bacteria which are differently distributed within the same plant harvested in different regionsItem Preventive effect of Xanthoria parietina polyphenols on the complications of diabetes in white rat(2019) Bouchenak, Ouahiba; Yahiaoui, Karima; Benhabyles, Narimen; Laoufi, Razika