Publications Scientifiques

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Author: search.filters.author.Haddad, NizarSubject: search.filters.subject.Algeria

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    Detection of paenibacillus larvae spores in honey samples from beekeepers of the central region of algeria
    (Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Sciences, 2013) Adjlane, Noureddine; Ameur lain, Karima; Gharabi, Nassiba; Lecksir, Nawel; Haddad, Nizar
    The American foulbrood in one of the most serious diseases that may affect brood of larvae and pupae stages, which cause economic losses and biological hazards in a large beekeeping sector in several countries across the world in general and Algeria in particular. The causative agent of this disease is a bacterium called Paenibacillus larvae that target bees Apis mellifera the latter are often present in honey.The aim of this project is studying the spread of this disease in the northern region of Algeria through the analysis of honey obtained from these areas. Microbiological, microscopic and biochemical methods were used in this study. The results obtained have shown that the prevalence rate varies from region to region, several factors may explain this variation in the prevalence of the disease. The average infection rate for all regions is 32%. To prevent the spread of this disease in Algeria must be taken is mandatory and means of prevention into account.
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    Etude de la sensibilité de la bactérie Paenibacillus larvae. agent causal de la loque américaine à l’antibiotique oxytétracycline
    (Université Badji Mokhtar- Annaba” (Algeria), 2016) Adjlane, Noureddine; Belkadi, Karima; Mecheri, Naima; Ridane, Hanane; Haddad, Nizar
    La loque Américaine est une pathologie très grave de l’abeille mellifere Apis melliffera dans le monde et en Algérie. Elle est causée par la bactérie Paenibacillus larvae. L’objectif de ce travail est d’évaluer la prévalence de la loque américaine dans la région centre d’Algérie (Alger, Bouira, Blida, Boumerdès, Tizi Ouzou, Tipaza, Medea, et Ain Defla) et de déterminer l’efficacité de l’oxytétracycline contre cette pathologie au laboratoire. Des tests microbiologique, biochimique et microscopique ont été utilisés pour évaluer la prévalence de cet agent pathogène. La majorité des régions étudiées sont infestées par cette pathologie avec un taux moyen de contamination de 47,56%. Nous avons établi le profil de résistance de 15 isolats vis-à-vis l’oxytetracycline par la méthode de diffusion par disque. Le classement des diamètres d’inhibition par ordre décroissant a montré que 8 sur 15 échantillons sont résistantes. Associées à une prophylaxie sanitaire, les antibiotiques peuvent permettre de diminuer les conséquences pathologiques de Paenibacillus larvae. Cependant, il est préférable d’alterner l’usage de différentes familles d’antibiotique afin d’éviter l’apparition de résistances
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    Prevalence of acariosis in honeybee colonies of Apis mellifera intermissa in Algeria
    (2018) Adjlane, Noureddine; Cherifi, Assia Habbi; Haddad, Nizar; Baz, Ahcen
    This study was aimed to investigate the prevalence of acariosis in honeybee colonies of Apis mellifera intermissa in Algeria. To this end, samples of bee colonies of the Apis mellifera intermissa race were taken from the brood frames for assessment of acariosis induced by Acarapis woodi in five provinces in Algeria viz., Tizi Ouzou, Blida, Boumerdès, Algiers, and Bouira. A sample of 100 to 150 bees spread over three to five colonies for each apiary was observed. Presence of this parasite was examined using laboratory and microscopic methods that entailed dissecting the thoraxes of the bees to expose their tracheas. Each trachea was examined under a microscope. In the case of the infected bees, the mites appear as small oval bodies attached to the transparent walls of the tracheas. According to the examination and analysis results, two cases of acariosis were observed in two colonies in the same apiary in the province of Boumerdès. In Bouira province, only one colony infested by the mite was found. Meantime, no single case of acariosis was detected in the provinces of Blida, Algiers, and Tizi Ouzou. These results indicate low prevalence of this disease in Algeria. For the infected apiaries, there is so far no 100% effective treatment for acariosis. Once this disease is present in the apiary, the beekeeper must control its development at a level that will not affect the health of the colony
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    Evaluation of oxalic acid treatments against the mite varroa destructor and secondary effects on honey bees apis mellifera
    (Tehran University of Medical Sciences, 2016) Adjlane, Noureddine; Tarek, El-Ounass; Haddad, Nizar
    Background: The Varroa destructor varroasis is a very serious parasite of honeybee Apis mellifera. The objective of this study was to evaluate the effectiveness of Varroa treatment using organic acid (oxalic acid) in Algeria identifying its side effects on bee colonies. Methods: Treatment was conducted in one apiary consisting 30 colonies kept in Langstroth hives kind. Oxalic acid dripped directly on bees 5ml of this solution of oxalic acid per lane occupied by a syringe. Three doses were tested: 4.2, 3.2 and 2.1% oxalic acid is 100, 75 and 50 g of oxalic acid dehydrate in one litter of sugar syrup (1water to1 surge) concentration. Results: The percentage of average efficiency obtained for the first dose was 81%, 72.19% for the second dose, and 65% for third one, while the dose of 100 g oxalic acid causes a weakening of honey bee colonies. Conclusion: The experiments revealed that clear variation in the treatment efficiency among colonies that this might be related to brood presence therefore in order to assure the treatment efficiency oxalic acid should be part of a big- ger strategy of Varroa treatment