Publications Scientifiques
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Item A Comprehensive Study on Crude Methanolic Extract of Daphne gnidium L. as Effective Corrosion Inhibitors of Mild Steel Induced by SRB Consortium(Springer, 2019) Djouahra-Fahem, Djamila; Angar, Yassmina; Lamine Mohamed, Gana; Khoukhi, Faiza; Kebbouche-Gana, SalimaThe aim of the present work is the evaluation of effect of methanolic extract obtained from Daphne gnidium against biocorrosion caused by sulphate-reducing bacteria (SRB). Herein, the study of the influence of SRB consortium has been realized on the biological and electrochemical properties of the carbon steel API5LX60 immersed in water sample obtained from an Algerian oil field separator. The monitoring of the treatment effects on the SRB performance using kits test and weight loss methods showed a positive effect of the methanolic extract of D. gnidium as a corrosion inhibitor at a concentration of 0.8 g/L. In the other hand, the weight loss test has generated an efficiency rate of 95.99% at a concentration of 1.6 g/L. A linear polarization resistance approved these results, and they have given a yield of 91.14% with a polarization resistance value of 28.9 kΩ cm2 at a concentration of 0.25 g/L.Item Biodegradation of Petroleum hydrocarbons and Biosurfactant production by an extremely halophilic Archaea Halovivax sp. A21(Aljest, 2017) Khemili-Talbi, S.; Kebbouche-Gana, Salima; Akmoussi-Toumi, S.; Gana, M.L.; Lahiani,S.; Ferrioune ,I.; Angar,Y.Over the recent few years, biosurfactant has played an important role in the industrial application especially in oil recovery; even in high salinity conditions. The potential of biosurfactant production by the extreme halophilic archaeon Halovivax sp. A21 in the presence of petroleum hydrocarbons (2% v/v) as sole carbon source at high salinity (25% NaCl) has been investigated. The results show the ability of Halovivax sp. A21 to grow and reduce surface tension under an optimum range of pH (7-9), salinities (15-35% NaCl) and temperature (40-45°C) for an optimized volume of 100 ml of the medium for 1000 ml capacity Erlenmeyer flasks with an optimum agitation of 120 rpm. The rates of biosurfactant production on petroleum hydrocarbons were enhanced with increasing NaCl concentration in the medium with an optimum of 25%. Biosurfactant production by Halovivax sp. A21 showed high emulsifying activity (more than 80%) and decreased surface tension (24.5 mN/m). The stability of the produced biosurfactant was determined by different physico-chemical conditions like pH, temperature and salinity. Moreover, the partial purification of the derived biosurfactant by silica gel column chromatography and Thin-layer chromatography revealed that it belongs to the lipopeptide group. Although both catechol dioxygenases participated in the degradation of petroleum hydrocarbons, more induction of catechol 1,2 dioxygenase was observed than the catechol 2,3 dioxygenase which indicated the predominance of the ortho cleavage pathways in the petroleum hydrocarbons degradation by the halophilic strain Halovivax sp. A21. The results demonstrated that strain Halovivax sp. A21 was able to increase the bioavailability of insoluble hydrocarbons, thus facilitating their uptake and their biodegradation even at high salt concentration. Likewise, the search of novel biosurfactants in extremophiles, or the use of microorganisms that present excellentItem Insights into the draft genome sequence of bioactives-producing Bacillus thuringiensis DNG9 isolated from Algerian soil-oil slough(2018) Daas, Mohamed Seghir; Rosana, Albert Remus R.; Acedo, Jeella Z.; Douzane, Malika; Nateche, Farida; Kebbouche-Gana, Salima; Vederas, John C.Bacillus thuringiensisis widely used as a bioinsecticide due to its ability to form parasporal crystals containingproteinaceous toxins. It is a member of theBacillus cereussensulato, a group with low genetic diversity butproduces several promising antimicrobial compounds.B. thuringiensisDNG9, isolated from an oil-contaminatedslough in Algeria, has strong antibacterial, antifungal and biosurfactant properties. Here, we report the 6.06 Mbpdraft genome sequence ofB. thuringiensisDNG9. The genome encodes several gene inventories for thebiosynthesis of bioactive compounds such as zwittermycin A, petrobactin, insecticidal toxins, polyhydroxyalkanoatesand multiple bacteriocins. We expect the genome information of strain DNG9 will provide another model system tostudy pathogenicity against insect pests, plant diseases, and antimicrobial compound mining and comparativephylogenesis among theBacillus cereussensulatogroupItem Kinetic and thermodynamic studies of the ammonium ions adsorption onto natural Algerian bentonite(Taylor & Francis, 2016) Angar, Yassmina; Djelali, Nacer-Eddine; Kebbouche-Gana, SalimaAdsorption processes are the most recommended complementary techniques applied in the wastewater treatment owing to their low economic cost, recyclability, efficiency and low power consumption. Clays known for this treatment are aluminosilicate minerals which contain different exchangeable ions on their surfaces. In the structure of bentonite surface, several cations have the exchange ability such as calcium, sodium, magnesium and potassium. This propriety is responsible for the adsorption of several cationic water pollutants. In this study, natural bentonite particles of 80 μm in diameter were considered and kept stirring at 200 RPM in a contaminated solution with ammonium ions. The effect of contact time and temperature showed that at an optimal temperature of 30°C and during a short stirring time of 50 min, the removal efficiency could reach its maximum of 53.36%. The kinetic study of the ammonium adsorption reaction on natural bentonite allowed concluding that the reaction is obeyed to the first-order model. Determination of various thermodynamic parameters suggests that the reaction is spontaneous and exothermicItem Purification and characterization of an organic solvent-tolerant and detergent-stable lipase from Haloferax mediterranei CNCMM 50101(Elsevier, 2018) Akmoussi-Toumi, Siham; Khemili-Talbi, Souad; Ferioune, Imen; Kebbouche-Gana, SalimaItem Draft genome sequence of bacillus paralicheniformis F47, isolated from an Algerian salty lake(American Society for Microbiology, 2018) Daas, Mohamed Seghir; Rosana, Albert Remus R.; Acedo, Jeella Z.; Douzane, Malika; Nateche, Farida; Kebbouche-Gana, Salima; Vederas, John C.Bacillus paralicheniformis F47 was isolated from a salty lake in Ain BaidaOuargla, southern Algeria. The genome contains genes for the production of several bioactive secondary metabolites, including the siderophore bacillibactin, the lipopeptides fengycin, surfactin, and lichenysin, the antibiotics bacitracin and kanosamine, and a putative circular bacteriocinItem Screening and biosurfactant/bioemulsifier production from a high-salt-tolerant halophilic cryptococcus strain YLF isolated from crude oil(Elsevier, 2017) Derguine-Mecheri, Louiza; Kebbouche-Gana, Salima; Khemili-Talbi, Souad; Djenane, DjamelItem Contribution to the study of the ammonium electro-oxidation in aqueous solution(Taylor & Francis, 2017) Angar, Yassmina; Djelali, Nacer-Eddine; Kebbouche-Gana, SalimaItem Investigation of ammonium adsorption on Algerian natural bentonite(Springer, 2016) Angar, Yassmina; Djelali, Nacer-Eddine; Kebbouche-Gana, SalimaItem Study of the effect of the bentonite modification treatments on the adsorption removal of ammonium ions from aqueous solution(2016) Angar, Yassmina; Djelali, Nacer-Eddine; Kebbouche-Gana, Salima