Publications Scientifiques

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Author: search.filters.author.Kebbouche-Gana, SalimaSubject: search.filters.subject.16S rRNA

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    Identification and phylogenetic analyses of anaerobic sulfidogenic bacteria in two Algerian oilfield water injection samples
    (Taylor & Francis, 2021) Lenchi, Nesrine; Kebbouche-Gana, Salima; Servais, Pierre; Gana, Mohammed Lamine; Llirós, Marc
    Corrosion of metallic oilfield pipelines by microorganisms is a costly but poorly understood phenomenon. For the first time, sulfidogenic communities in injection waters of two Algerian oilfields, Tin Fuin Tabankort (IT3) and Stah (IS2) were examined using the 16S rRNA gene cloning and sequencing approach. Water samples were inoculated into selective medium for sulfate-reducing bacteria and incubated under anaerobic conditions at 45 °C. The total number of culturable sulfidogenic microorganisms in the samples obtained from the two sampled waters (IT3 and IS2) was 2.4 × 105 cells/mL and 3.9 × 104 cells/mL, respectively. Scanning electron microscopy analyses showed different morphological forms reflecting the diversity of sulfidogenic communities. 16S rRNA gene sequencing and phylogenetic diversity analyses revealed that both water reservoirs harbor large amounts of anaerobic bacteria. However, a majority of all the sequences analyzed (e.g., 34% in the IS2 and 84% in the IT3 samples) were not assigned to any known bacterial group, suggesting that subsurface waters harbor very large sulfidogenic anaerobic microbial communities of as yet undescribed bacterial phyla. Proteobacteria were found to be the most dominant phylum in the IS2 sample (49%); however, no Proteobacteria were detected at the IT3 production well. The Firmicutes phylum (10%) was detected in the two water samples, whereas Bacteroidetes phylum (7%) was retrieved only in IT3. The most abundant related genera were: Desulfotomaculum, Porphyrobacter, Hyphomicrobium, Acidocella, Comamonas, Ramlibacter, Pseudomonas, Enterobacter and Flavitalea. No shared operational taxonomic units were observed among the two samples analyzed, demonstrating the uniqueness of each subsurface water well. This study demonstrates the diversity of the sulfidogenic bacteria that might play a critical role in the souring mediated corrosion of metallic oilfield pipelines. This information could help oilfield companies develop better anticorrosion treatments and strategies
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    Diversity of Culturable Halophilic Archaea and Bacteria from Chott Tinsilt and El Malah Salt-Lake in Algeria
    (Science publications, 2020) Akmoussi-Toumi, Siham; Khemili-Talbi, Souad; Kebbouche-Gana, Salima; Lenchi-Izouine, Nesrine; Khelfaoui, Mohamed El Amine; Sayah, Amna; Bouarab, Ghania; Ferrioune, Imen; Mokhtari, Wafa; Najjari, Afef
    At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these ecosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced hydrolases at high salt concentrations. The Production yields obtained are very promising for applications in the biotechnology and industrial microbiology.
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    Diversity of culturable halophilic archaea and bacteria from chott tinsilt and el malah salt-lake in Algeria
    (Current Research in Bioinformatics, 2020) Akmoussi-Toumi, Siham; Khemili-Talbi, Souad; Kebbouche-Gana, Salima
    At the limits of life, hyper-saline aquatic ecosystems; Chott and Sebkha are a model of choice of extreme environments, housing a halophilic microflora that had to adapt to these conditions. In Algeria, these cosystems are poorly studied. However, our study was carried out on the waters of Chott Tinsilt and Sebkha El Malah. The study of this microflora revealed the presence of a significant morphological, physiological and metabolic diversity. The molecular study allowed us to access to a phylogenetic affiliation including an Archean Species (ATS1) and 7 bacterial species (A1, A2, A3, A4, B1, B4, B5). The results showed that these isolates were related to the genera Haloferax (for the strain ATS1) and Halomonas (strains A1, A2 and A4), Staphylococcus (strain A3), Salinivibrio (strain B1), Planococcus (strain B4) and Halobacillus (strain B5). Most isolates produced ydrolases at high salt concentrations. The Production yields obtained are very promising for pplications in the biotechnology and industrial microbiology.