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Subject: search.filters.subject.Bacillus thuringiensis

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    Efficient and cost-effective production of Bacillus thuringiensis subsp. aizawai spores and delta-endotoxins using agricultural raw materials and agro-industrial wastes under submerged fermentation
    (Elsevier, 2022) Gounina-Allouane, Rabia; Acheuk, Fatma; Sahir-Halouane, Fatma
    In this study, cost-effective liquid culture media based on agricultural raw materials and agro-industrial wastes were developed for spores and delta-endotoxins production by a strain of B. thuringiensis subsp. aizawai used as a larvicide in wax moths control. The raw materials used include edible leguminous seeds, corn, wheat bran, dry palm date pulps, and corn forages. The agro-industrial wastes were dry palm dates pits and olive mill solid wastes. The protein and carbohydrate contents of the developed media were measured. Sporulation on the different developed media was monitored and delta-endotoxins produced were quantified. Mono-component media containing chickpea, red lentils, and wheat bran produced a spore count compared to the reference medium NYSM. The results showed that the dry palm date's pulp extract was the most efficient medium for spore production (1.43 × 1010 spores ml−1), and the chickpea medium was the most efficient for delta-endotoxins production
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    Insights into the draft genome sequence of bioactives-producing Bacillus thuringiensis DNG9 isolated from Algerian soil-oil slough
    (2018) Daas, Mohamed Seghir; Rosana, Albert Remus R.; Acedo, Jeella Z.; Douzane, Malika; Nateche, Farida; Kebbouche-Gana, Salima; Vederas, John C.
    Bacillus thuringiensisis widely used as a bioinsecticide due to its ability to form parasporal crystals containingproteinaceous toxins. It is a member of theBacillus cereussensulato, a group with low genetic diversity butproduces several promising antimicrobial compounds.B. thuringiensisDNG9, isolated from an oil-contaminatedslough in Algeria, has strong antibacterial, antifungal and biosurfactant properties. Here, we report the 6.06 Mbpdraft genome sequence ofB. thuringiensisDNG9. The genome encodes several gene inventories for thebiosynthesis of bioactive compounds such as zwittermycin A, petrobactin, insecticidal toxins, polyhydroxyalkanoatesand multiple bacteriocins. We expect the genome information of strain DNG9 will provide another model system tostudy pathogenicity against insect pests, plant diseases, and antimicrobial compound mining and comparativephylogenesis among theBacillus cereussensulatogroup