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Subject: search.filters.subject.Endophytic bacteria

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    Characterization of non-rhizobial endophytic bacteria in algerian cowpea root nodules: unveiling potential for nitrogen fixation
    (Universitatea din Oradea, 2024) Benlahrech, Samia; Boucelha, Lilya; Cherchali, Amina; Djebbar, Réda; Ounane, Sidi Mohamed
    The study aimed to characterize the culturable endophytic bacteria present in cowpea nodules (Vigna unguiculata L. (Walp)). Five endophytic non-rhizobial isolates (SVU1, SVU3, SVU9, SUV15, and SUV18) were isolated from the root nodules of cowpea. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates belonged to the genus Paenibacillus, Serratia, Microbacterium, Bacillus and Lysinibacillus. Unlike rhizobia, all five isolates absorbed Congo Red contained in the Yeast Mannitol Extract medium. The nitrate reductase test was positive for all strains except SVU18and all strains utilized, mannitol, inositol and sucrose as substrates. The oxidase reductase test was negative. The results indicated that the root nodules of the legume could host a diverse endophytic community associated with cowpea, representing the first report of endophytic bacteria from Algerian cowpea nodules. These findings shed light on a part of the diversity of non-rhizobial within the cowpea nodules. The findings about the nifH gene showed that these bacteria open avenues for further exploration of the potential applications of endophytic bacteria in promoting nitrogen fixation and enhancing plant growth. Future studies will explore the potential application of these strains in nitrogen fixation and growth promotion for sustainable agriculture.
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    MALDI-TOF MS detection of endophytic bacteria associated with great nettle (Urtica dioica L.), grown in Algeria
    (2018) Toubal, Souheyla; Bouchenak, Ouahiba; Elhaddad, Djillali; Yahiaoui, Karima; Boumaza, Sarah
    Any plant with a vascular system has a specific endophytic microflora. The identification of bacteria is essential in plant pathology. Although identification methods are effective, they are costly and time consuming. The purpose of this work is to isolate and to identify the different bacteria from the internal tissues of Urtica dioica L. and to study their diversity. This last is based on the different parts of the plant (stems, leaves and roots) and the harvest regions (Dellys and Tlamcen). The identification of bacteria is done by biochemical tests and confirmed by MALDI-TOF MS. Seven genus and eleven species were isolated from the Great Nettle. They belong to the genera Bacillus, Escherichia, Pantoea, Enterobacter, Staphylococcus, Enterococcus and Paenibacillus. The majority of these bacteria were isolated from Tlemcen which makes this region the richest in endophytic bacteria compared to that harvested from Dellys. The results show also that the leaves are the most diversified in endophytic bacteria. Bacillus pumilus-ME is the common species of the three parts of the plant harvested in both regions. From this work, it emerges that the Great Nettle can be settled by various endophytic bacteria which are differently distributed within the same plant harvested in different regions