Browsing by Author "Khoury, Fares"

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Diagnosis of paenibacillus larvae from honeybees in Jordan according to microbiological and chemicals techniques
(Academic Journals Inc, 2015) Haddad, Nizar; Al-tellawi, Alaa; Adjlane, Noureddine; Khoury, Fares; Quddoumi, Samar
American Foulbrood Disease (AFB) is considered one of the most virulent bacterial diseases of honeybee (Apis mellifera); it has a vital negative impact on the beekeeping industry worldwide. This work includes the subsequent diagnosis including; chemical, microbiological procedure for detection of Paenibacillus larvae. During the spring and the summer of 2009, fifty-six honey and larval samples were collected from 56 inspected honeybees colonies located in 53 apiaries representing beekeeping all over Jordan. The samples were examined to assess the presence of Paenibacillus larvae in honey samples by using both chemical and microbiological methods. Honey bees field diagnosis procedures were considered to be the first diagnosis step for AFB, which can be done by the beekeepers themselves. The total of 57 (honey brood and brood nest honey) from different regions of Jordan was inspected to carry P. larvae spores with 35%. The percent referred to the ability of Jordanian beekeepers to ascertained AFB symptoms. American foulbrood exists in Jordan with different distribution depending on the environmental condition. This pathology can be best detected by isolation of Paenibacillus larvae from Brood-nest honey samples
Diagnosis of Paenibacillus larvae from Honeybees in Jordan According to Microbiological and Chemicals Techniques
(Asian Network for Scientific Information, 2016) Jamal Haddad, Nizar; Tellawi, A .; Adjlane, Noureddine; Khoury, Fares
American foulbrood disease (AFB) is considered one of the most virulent bacterial diseasesof honeybee (ApismelliferaLinnaeus, 1758)it has a vital negative impact on the beekeeping industry worldwide. This disease is caused by a spore forming bacterium Paenibacillus larvae that affects honeybee larvae. This work includes the subsequent diagnosis including microbiological and PCR procedure for detection of P.larvae. Fifty-six honey and larval samples were collected from 56 inspected honeybee colonies located in 53 apiaries representing beekeeping all over Jordan. The samples were examined to assess the presence of Paenibacillus larvae in honey samples by using both microbiological methods and PCR techniques.Paenibacillus larvae was isolated from 41.6% samples (honey and larval) using bacteriological methods and 38.5% using PCR techniques. PCR were effectively used to amplify a specific region of the genome of P.larvae from pure culture isolates, honey and larvae samples; amplified regions include: Enterobacterial Repetitive Intergenic Consensus (ERIC) and 35KDa Metalloprotease. The positive PCR samples were collected in Amman, Irbid, Al-Ghor, Al-Zarqa and Al-Balqa
Screening the Efficacy of Different Anti-biotic Against American Foulbrood in Jordan
(2016) Jamal Haddad, Nizar; Khoury, Fares; Tellawi, Alaa; Adjlane, Noureddine
American foulbrood disease (AFB) is considered one of the most virulent bacterial diseases of honeybee (Apis mellifera); it has a vital negative impact on the beekeeping industry worldwide. This disease is caused by a spore forming bacterium Peanibacillus larvae that affects honeybee larvae. This work includes the screening of several alternative antibiotics efficacy to AFB disease. The sensitivity of Peanibacillus larvae to seven antibiotics was investigated using disk diffusion methods (Kirby Bauer disk diffusion). The tested compounds are approved by the US-Food and Drug Administration for agricultures uses. The most active antibiotics were Novobiocin and Florfenicol followed by Oxytetracycline and Tylosin. Erythromycin and Tilmicosin had intermediate activity; Lincomycin was recorded as the lowest active