Screening the Efficacy of Different Anti-biotic Against American Foulbrood in Jordan

Abstract

American foulbrood disease (AFB) is considered one of the most virulent bacterial diseases of honeybee (Apis mellifera); it has a vital negative impact on the beekeeping industry worldwide. This disease is caused by a spore forming bacterium Peanibacillus larvae that affects honeybee larvae. This work includes the screening of several alternative antibiotics efficacy to AFB disease. The sensitivity of Peanibacillus larvae to seven antibiotics was investigated using disk diffusion methods (Kirby Bauer disk diffusion). The tested compounds are approved by the US-Food and Drug Administration for agricultures uses. The most active antibiotics were Novobiocin and Florfenicol followed by Oxytetracycline and Tylosin. Erythromycin and Tilmicosin had intermediate activity; Lincomycin was recorded as the lowest active