Publications Internationales

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Author: search.filters.author.Adjlane, NoureddineSubject: search.filters.subject.Honey bee

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    Detection of deformed wing virus in the local bee colonies apis mellifera intermissa in algeria and its relationship with varroa destructor
    (2014) Adjlane, Noureddine; Jamal Haddad, Nizar
    Honeybees are threatened by over 18 viruses, nowadays deformed wing virus (DWV) is known to be one of the most prevalent virus worldwide.. This is the first study in Algeria and most North Africa region which is evaluating the prevalence of the DWV in beehives. The objective of this study is to eva-luate the impact of this virus on honey bees mortality, and its relationship to infestation with the Varroa destrutor parasitic mite. We conducted this study on an apiary located in the central region of Algeria. PCR results showed the presence of DWV in Apis mellifera intermissa honey bee colonies, where 42% of the samples are infected with the virus. We highlighted the role of V. destructor and its association with the DWV and the mortality recorded in the same apiary
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    Effect of Some Honeybee Diseases on Seasonal Mortality ofApismellifera intermissain Algeria Apiaries
    (Springer, 2018) Adjlane, Noureddine; Jamal Haddad, Nizar
    With a view to identify the pathogens and to establish the role of these pathogens in regulation of the density of honey bee population occurring in the apiaries of the area concerned samples of honeybee were collected from the beekeepers in some parts of central Algeria It is revealed that Nosema sp., Varroa destrutor, Peanibacillus larvae are associated with the disease manifestation in honey bees. The presence of Nosema sp., Varroa destrutor, Peanibacillus larvae was analyzed using standard OIE methods. Spores of Paenibacillus larvae were detected in 56.6 % in winter 52.32 % in spring. 29.33 % in autumn and 11.25 % in summer. Nosema infestation was recorded in 47.91 % bee individuals during spring. Varroa infestation rate was maximum 12.57 % in summer and lowest 3.44 % in spring. Analysis of data indicates that Boumerdes and Tipaza, diseases induced mortality exceeds 10 % in honeybee. There exists a significant correlation between Nosema disease and mortalities in honeybees. Seasons play significant role, irrespective of pathogens, in disease manifestation
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    Next-generation sequence data demonstrate several pathogenic bee viruses in Middle East and African honey bee subspecies (Apis mellifera syriaca, Apis mellifera intermissa) as well as their cohabiting pathogenic mites (Varroa destructor)
    (Springer link, 2018) Haddad, Nizar Jamal; Horth, Lisa; Alshagour, Banan; Adjlane, Noureddine
    RNA viruses are associated with honey bee (Apis mellifera) colony losses in many parts of the world. Their consequences may be exacerbated when the ectoparasite mite Varroa destructor is present in hives. While evidence of pathogenic, viral-induced disease is abundant in western honey bees (Apis mellifera mellifera) from many parts of the world, less information exists regarding the pathogen load of Apis mellifera syriaca and Apis mellifera intermissa, honey bees from the Middle East and North Africa (MENA) that play substantial roles in regional beekeeping. Here, we used next-generation sequencing to evaluate the viral populations of these subspecies and their associated mites. We found that both A. m. syriaca and A. m. intermissa, as well as the Varroa mites infecting their colonies, bear a suite of RNA viruses including major pathogenic viruses like Deformed wing virus, Acute bee paralysis virus, Black queen cell virus and Sacbrood virus, and less common viruses (e.g., bee Macula-like virus and Apis mellifera filamentous virus). The two native honey bee MENA subspecies have acquired different but overlapping suites of pathogens, which also differ, but overlap, with the suites detected in the mites. The presence of plant viruses suggests that they were acquired from foraging for pollen and nectar. Phylogenetic analysis of the above common pathogenic RNA viruses showed unexpected genetic relationships with other known strains, indicative of import to MENA from outside of the region. Our findings indicate that it is important to carefully consider the impact of the movement of queens and mobile colonies, and the effects such movement have, on the transmission of disease
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    Study of the Efficacy of Oxalic Acid and Thymovar (Thymol) against the Parasitic Mite of the Honey Bee, Varroa destrutor
    (Egyptian Academic Journal of Biological Sciences. A, Entomology, 2020) Adjlane, Noureddine; Smaoui, Redha; Jamal Haddad, Nizar
    Varroasis is a dangerous pathogen of the honey bee caused by the mite Varroa destructor, the aim of this work is to assess the effectiveness of two natural products (oxalic acid and thymol) in the fight against varroasis. The experiment covered 40 colonies in the central region of Algeria. Two doses of oxalic acid were used: 30 and 45 grams per liter of sugar syrup. For thymol (Thymovar), two treatments were carried out: ½ strip with one and two applications for each group of colonies. The 1/2 strip formulation in two applications at an interval of two weeks apart seems the most effective; the 45 gm solution of oxalic acid has a higher efficacy compared to that of 30 gm, that the effectiveness of oxalic acid is directly linked to the presence of brood at the time of the intervention. these two treatments are of control measure to be integrated by beekeepers as part of an alternative control strategy
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    Diagnosis of paenibacillus larvae from honeybees in Jordan according to microbiological and chemicals techniques
    (Academic Journals Inc, 2015) Haddad, Nizar; Al-tellawi, Alaa; Adjlane, Noureddine; Khoury, Fares; Quddoumi, Samar
    American Foulbrood Disease (AFB) is considered one of the most virulent bacterial diseases of honeybee (Apis mellifera); it has a vital negative impact on the beekeeping industry worldwide. This work includes the subsequent diagnosis including; chemical, microbiological procedure for detection of Paenibacillus larvae. During the spring and the summer of 2009, fifty-six honey and larval samples were collected from 56 inspected honeybees colonies located in 53 apiaries representing beekeeping all over Jordan. The samples were examined to assess the presence of Paenibacillus larvae in honey samples by using both chemical and microbiological methods. Honey bees field diagnosis procedures were considered to be the first diagnosis step for AFB, which can be done by the beekeepers themselves. The total of 57 (honey brood and brood nest honey) from different regions of Jordan was inspected to carry P. larvae spores with 35%. The percent referred to the ability of Jordanian beekeepers to ascertained AFB symptoms. American foulbrood exists in Jordan with different distribution depending on the environmental condition. This pathology can be best detected by isolation of Paenibacillus larvae from Brood-nest honey samples