Publications Internationales

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    Chemical composition and bioactivity of essential oil against the green peach aphid (Myzus persicae)
    (Springer, 2022) Dehliz, Abderrahmene; Lakhdari, Wassima; Mlik, Randa; Chahbar, Nora; Acheuk, Fatma; Mekhadmi, Nour El Houda; Benyahia, Ibtissem; Fethallah, Rabab; Hammi, Hamida; Berrekbia, Mohammed; Badjadi, Zeineb
    The present experiment was conducted to elucidate the chemical composition of essential oil (EO) of a native spontaneous plant, Cotula cinerea Del., in southeastern Algeria and to evaluate its insecticidal effect on the Green Peach Aphid (GPA) Myzus persicae Sulzer. GC–MS and GC-FID analyses showed that this plant was characterized by 35 components, representing eight major constituents, i.e., santolina triene (11.55%), sabinene (5.86%), cineol (6.21%), α-thujone (22.91%), (R)-camphor (7.50%), cis-sabinyl acetate (6.41%), carveol (7.68%), and ipsenol (4.60%). The considered essential oil was characterized by a clear predominance of hydrocarbon compounds chiefly monoterpenes (35.63%), oxygenated monoterpenes (64%), and sesquiterpenes (0.35%). Insecticidal assays showed that the EO was toxic against M. persicae adults recording 75.95% of mortality in the 3rd dose with a lethal concentration (LC50) equal to 302.4 µl where the lethal time (LT50) was 91.6 h. Also, it has a significant repellency showing a high rate in the 2nd dose (64.6%) with a highly significant difference (p = 0.000). As well as its inhibitory effect on the egg-laying where it has greatly reduced the reproduction potential of M. persicae adults. Obtained data support the possibility to develop C. cinerea oil, especially component specification, as a new natural bio-insecticide
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    Inhibitory effect of Trichoderma harzianum on mycelial growth of Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani
    (Springer Netherlands, 2018) Lakhdari, Wassima; Dehliz, Abderrahmene; Mlik, Randa; Hammi, Hamida; Benlamoudi, Wiam; Acheuk, Fatma; Doumandji-Mitiche, Bahia B.
    Tomato is one of the most important fruit vegetables for human consumption in Algeria. In recent years, the cultivation of this plant is experiencing a very rapid development. However, this speculation is confronted with several problems including plant health. The purpose of this study is to find biological ways to introduce them into an integrated control program against tomato pests and have an organic agriculture that can increase the production of this culture. Direct confrontation test of an indigenous strain of Trichoderma harzianum with two phytopathogenic fungi that are isolated from tomato leaves (Fusarium oxysporum f. sp. radicis-lycopersici and Alternaria solani) was carried at 26 °C on an artificial culture medium (PDA). It could be demonstrated that T. harzianum inhibited mycelia growth of the two fungi with more than 40% and only after 4 days of incubation. Statistical analysis confirms that T. harzianum had a significant effect of antagonism against the tested pathogens mentioned before with P value 0.02 and 0.04, respectively. Beyond this period, and at the end of the sixth day, T. harzianum occupied the colonies of fungus and began to sporulate marking a very important myco-parasitic power. Indeed, a percent inhibition greater than 50% was recorded into the both fungi