Publications Internationales
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Item Effects of pollen deprivation in groups of tellian (Apis mellifera intermissa) and saharan (Apis mellifera sahariensis) honey bees under controlled conditions(MDPI, 2022) Khedidji, Hassiba; Abderrahmani, Khaled; Oulebsir-Mohandkaci, Hakima; Ladjali-Mohammedi, Kafia; Mohammedi, ArezkiWorldwide, honey bees are increasingly faced with periods of pollen scarcity, which can lead to nutritional deficiencies, especially of proteins and amino acids. These are essential for the proper functioning of the single organism and the colony. To understand how bees react to protein deficiency, under controlled conditions, we studied the effect of pollen deficiency on the main physiological parameters in two subspecies endemic of Algeria, Apis mellifera intermissa and Apismellifera sahariensis. Emerging workers of both subspecies were reared with two diets: one was pollen-fed, whereas the other pollen-deprived. Several physiological criteria were measured depending on the type of diet and subspecies: the survival of the bees, the amount of total protein in the hemolymph, hypopharyngeal glands development and the ovary development of workers. These last three parameters were assessed at three different ages (7, 14 and 21 days). At birth, sahariensis workers weighed more than intermissa. With the same protein diet, the average life expectancy of sahariensis was extended by 5.55 days compared to intermissa. Even if deprived of pollen, sahariensis lived longer than intermissa fed with pollen (p < 0.001). In the three age levels, the hypopharyngeal glands were more developed and less affected by pollen deficiency (p < 0.001) in sahariensis than in intermissa (p < 0.001). The total hemolymph protein was higher in intermissa than in sahariensis regardless of the diet, and was also higher in protein-fed than in deprived bees (p < 0.001). The ovaries developed more rapidly with a high proportion in intermissa than in sahariensis (p < 0.05) regardless of the diet, and was also higher in the bees fed with pollen than those deprived (p < 0.05). Pollen deficiency generates physiological alterations and modifications, the amplitude of which varied according to the subspecies of the bee studiedItem Isolation of entomopathogenic microorganisms from soil: study of the pathogenicity of an Aspergillus strain and its toxins against Galleria mellonella(2020) Oulebsir-MohandKac, Hakima; Benzina-Tihar, Farida; Reghmit, Abdenacer; Lahian, Sadjia; Mohammedi, Arezki; Salhi, Khadidja; Salh, WidadThis study aims at finding an alternative to control bee enemies in order to protect this precious insect. Entomopathogenic microorganisms are isolated from the rhizosphere, and an Aspergillus strain was chosen to be tested against the wax moth Galleria mellonella and the poliniphagous beetle Trogoderma sp. The work of the first part was oriented towards the isolation of bacteria and fungi from soil and phenotypic identification of the fungal strain chosen. Then production and characterization of toxins produced by Aspergillus sp. by submerged fermentation using Maltose medium were done. The double extraction of the supernatant with dichloromethane and its separation by TLC allowed us to reveal the production of Ochratoxin A and Fumagillin. Biotests using different fungal suspensions (D1 = 5.2 * 10 3 sp/ml; D2 = 5.2 * 10 5 sp/ml, D3 = 5.2 * 10 7 sp/ml) and mycotoxins against the two pests allowed us to observe that the treatment did not affect Trogoderma beetle. By contrast, the wox moth was infected by the fungus. This result was confirmed by the qualitative and quantitative determination of the haemolymphatic metabolites which revealed a clear disturbance. The verification of the harmlessness of the fungus tested against *Corresponding author h.mohandkaci@univ-boumerdes.dz Apis mellifera showed that the highest dose 10 7 spores/ml is moderately toxic in honey bees. Finally, the application of minimum inhibitory doses of biocontrol agent Aspergillus will allow to avoiding possible mortalities to beesItem Differences in grooming behavior between susceptible and resistant honey bee colonies after 13 years of natural selection(Springer Nature, 2020) Dadoun, Nedjma; Nait-Mouloud, Mohamed; Mohammedi, Arezki; Sadeddine Zennouche, OurdiaThe present study was conducted to quantify at the individual level the grooming behavior of bees from resistant colonies and susceptible colonies. Experienced and naive bees from resistant colonies were compared to experienced and naive bees from susceptible colonies at the age of 4, 7, 15 and 21 days. In a total of 480 assays, resistant bees successfully groomed off 10 times more mites placed than susceptible bees. Worker of different ages are involved but the lowest percentage of grooming was observed in 21-day-old bees. The experienced bees from resistant colonies bees that have evolved in a natural environment removed significantly more mites (69.2%) compared to naive bees (51.7%) who had no contact with other older bees.Item Antimicrobial potential of ziziphus and euphorbia honeys harvested in semi-arid region of Algeria and their possible use in soft medicine(EBSCO, 2020) Haderbache, Latifa; Annou, Saada; Mohammedi, ArezkiTwo different botanical origin honey types (Ziziphus lotus and Euphorbia bupleuroides) from semi-arid regions in Algeria consisting of twelve samples were tested for their antimicrobial efficiency. Global assessment of antimicrobial activity was made by wells method on integer samples and by turbidity test to locate fraction responsible of this activity. Honeys have been tested against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Fungal strain was resistant to all honeys at all concentrations, whereas E. coli and S. aureus were sensitive presenting minimum inhibition concentrations (MIC) between 10 and 50%. Euphorbia honeys appeared to be more active. The fractionation shows that volatile fraction can have great antimicrobial effect, followed by the acidic one. Correlations reveal good relation between inhibitory effect, free acidity and polyphenols. These facts show large possibilities for honey use in soft medicine against some bacterial infections. Copyright of Journal of Microbiology, Biotechnology & Food Sciences is the property of Slovak University of Agriculture in Nitra, Faculty of Biotechnology & Food Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstractItem Etude sur le comportement de consommation du miel en Algérie : attentes et préférences(2015) Haderbache, Latifa; Mohammedi, ArezkiCette étude tente de dresser un portrait, dans le contexte actuel de la filière apicole, de la perception du consommateur algérien du miel, de ses attentes et de ses préférences. Une enquête de consommation a été enclenchée sur une population de 800 répondants (questionnaire) de 20 wilayates. Les résultats montrent que l’algérien consomme environ 0,200 kg/an/h. Ces niveaux de consommations restent très faibles, donnant au miel une part insignifiante du régime alimentaire comparé à tous les bienfaits qu’il en attend. Le consommateur algérien considère le miel comme médicament, ses préférences se portent sur les miels foncés, liquides, emballés dans des bocaux en verre, mais il est dans la confusion totale quant au circuit commercial de celui-ci ; de ce fait, il se procure ses miels par les circuits informels et par des rapports de confiance ou se tourne vers les miels d’importation présents dans les commerces, Sans être sure de la qualité de ces produitsItem Ziziphus Lotus and Euphorbia bupleuroides Algerian Honeys(IDOSI Publications, 2013) Haderbache, Latifa; Bousdira, Mouna; Mohammedi, ArezkiTwo Algerian honey types of semi-arid regions are characterized. Botanical origin is controlled by qualitative and quantitative melissopalynology. Based on dendrograms test, the 27 studied samples were classified into three homogeneous groups. The control analyzes showed that they are of good quality in agreement with the Codex Alimentarius. For discriminating between groups, physico-chemical parameters and phenolic and sugar composition were measured. But it is only the pH, free acidity, electrical conductivity and specific rotation that were powerful to differentiate unifloral Ziziphus Lotus group, Euphorbia and multiforal honeys containing Ziziphus . Ziziphus lotus honey presented specific characteristics, with high pH (4.96 ± 0.91), dextrogyrous specific rotation (-1.41 ± 7.55°) and high diastase activity (36.06 ± 10.06). While, Euphorbia honeys were within honey standards, with poor proline content (114.3 ± 30.3ppm) and low Diastase activity (14.6 ± 4.1Ush).The mineral fraction of ziziphus honey reveals the predominance of potassium, calcium and sodium with a constant presence of iron and magnesium. Finally, correlations analysis suggests the existence of a strong relationship between color and flavonoïd fraction, which is present in significant amounts (15-30 mg EQ/100g) compared to rates usually found in European monofloral honeys (1-6 mg EQ/100g), which is remarquable