Publications Internationales

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Author: search.filters.author.Oukali, ZahiaHas files: No

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    Insecticidal and Histological Effects of Extracts from Leaves of Juglans regia L. Against Sitophilus oryzae (Coleoptera: Curculionidae) Isolated from Soft Wheat in Post-harvest
    (Springer Science and Business Media Deutschland GmbH, 2025) Bennacer, Amel; Sahir-Halouane, Fatma; Smaili, Oussama; Oukali, Zahia; Mouhouche, Faiza; Bennacer, Nour El Houda; Chaib, Ratiba; Kumar, Ramar Muhan
    In Algeria, the cultivation of soft wheat (Triticum aestivum Linn.) plays a crucial role in the food chain. However, the crop faces a threat from Sitophilus oryzae (Coleoptera: Curculionidae), which causes substantial economic losses. This study aims to discover novel bioactive compounds derived from Juglans regia Linn., a walnut tree grown in Algeria, to combat S. oryzae. This study focuses on extraction, phytochemical characterization, insecticidal effect against S. oryzae adults, and histological impact. GCMS analysis of the petroleum ether (EPJ) and chloroform (CHJ) fractions indicated the presence of a diverse range of bioactive molecules, including myrtenal (1.17%), eugenol (46.17%), methyl ester (25.84%), lycophyll (5.16%), and hexadecane (1.22%), which are derived from terpenoids, phenols, and fatty acids. LCMS/MS analysis of the ethyl acetate (AEJ) and butanolic (BTJ) fractions identified compounds mainly associated with hydrocinnamic acids, flavonols, flavones, and naphtoquinones, such as 3-caffeoylquinic acid, epi-catechin isomer, kaempferol-3-glucoside, and hydro-juglone derivative. Furthermore, the efficacy of the five extracts (hydromethanolic extract and the four fractions) against S. oryzae adults was evaluated at 625, 315, and 75 µg/mL concentrations over 7 days. EPJ and BTJ demonstrated the highest effectiveness among these extracts, as indicated by their relatively low LC50, LC90, LT50, and LT90 values. These results highlight the potential of EPJ and BTJ, derived from walnut leaves, as biocontrol agents against S. oryzae. Histological analysis of the treated insects revealed significant alterations in various tissues compared to untreated adults. This research gives the first evidence that J. regia grown in Algeria is a promising biocontrol agent against pest beetles in stored soft wheat.
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    Structural characterization of phytochemical content, antibacterial, and antifungal activities of Juglans regia L. leaves cultivated in Algeria
    (Elsevier, 2022) Bennacer, Amel; Sahir-Halouane, Fatma; Aitslimane-Aitkaki, Sabrina; Oukali, Zahia; Oliveira, Ivo Vaz; Rahmouni, Naima; Aissaoui, Mohammed
    Many microorganisms cause diseases to plants, animals and humans. Recently, natural compounds extracted from different medicinal and officinal plants have had a promising antimicrobial potential. In this present study, the antifungal and antibacterial activities of Juglans regia leaves cultivated in Algeria have been evaluated. The chemical composition was analyzed by GCMS spectroscopy. Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, Potassium iodide and vanilline methods respectively. The antibacterial activity was tested against five bacterial strains and the antifungal activity was tested against seven fungal strains. The GC-MS profile of ethyl acetate fraction (tannic extract) revealed the presence of many interesting biomolecules we note: Phenol (0.19%), gaiucol (0.16%), catechol (0.65%), 2-Methoxy-4-vinylphenol (1.77%), eugenol (0.78%), (E)-coniferol (0.66%), 1, 5-naphthalenediol (2.01%) and 5-hydroxy-1, 4-naphthalenedione or juglone (0.18%). The tannic extract (ethyl acetate fraction) presented a high antifungal activity on Aspergillus terreus, Aspergillus ochraceus and Aspergillus Brasilliensis ATCC 16404 with percentage of growth inhibition of 77,033 ± 3,395, 73,327 ± 3,851 and 71,850 ± 0,641 respectively for the highest concentration. It also showed an interesting antibacterial activity against Klebseilla pneumoniae ATCC 70063 with minimal inhibitory concentration of 3.125 μg/ml comparing to the hydro-acetonic extract(MIC value). Our finding provides for the first time the chemical profiling of this species grown in Algeria and also an efficient and targeted natural approach to the discovery of classes of antibacterial and antifungal compounds for various applications