Publications Internationales

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Subject: search.filters.subject.Antimicrobial activity

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    Impact of gamma irradiation on phytochemical composition, and biological activities of Lepidium sativum seeds extract
    (Walter de Gruyter GmbH, 2024) Hadj Rabia, Samia; Debib, Aicha; Eddaikra, Atika; Aberkane-Mchebbek, Lila; Nouri, Ramy; Benmoussa, Fatima; Mokhtari, Amine; Medjber, Sarah; Mansouri, Belkacem; Messaoudi, Mohammed
    The main objective of this research is to assess how gamma radiation influences the chemical composition and activities such as antioxidant, antimicrobial and anticancer activities of Lepidium sativum (L. sativum) seeds. Methanolic extracts were obtained through a 24 h maceration process from L. sativum seeds exposed to various doses of gamma irradiation. Phytochemical screening involved precipitation and staining reactions, with the antioxidant potential assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Antimicrobial activity was evaluated through a diffusion test on agar medium, while the cytotoxic potential on the lung cancer line “A-549 cells” was examined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Qualitative analysis of the extracts identified preserved bioactive constituents after irradiation. Additionally, quantitative analysis indicated a significant rise in the total content of polyphenols and flavonoids under radiation, accompanied by an enhanced reducing power compared to the control. The antimicrobial and cytotoxic capabilities also improved, evident in increased growth inhibition zones for certain microbial strains and reduced viability rates of A549 cells. These results show promise and warrant further investigation into the potential of gamma radiation to enhance additional biological effects of plants.
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    Coordination of new palladium (II) complexes with derived furopyran-3,4‑dione ligands: Synthesis, characterization, redox behaviour, DFT, antimicrobial activity, molecular docking and ADMET studies
    (Elsevier, 2022) Fahima Dechouk, Lamia; Bouchoucha, Afaf; Abdi, Yamina; Si Larbi, Karima; Bouzaheur, Amal; Terrachet-Bouaziz, Souhila
    The synthesis, characterization, theoretical study, electrochemical behaviour and biological activity of new series of Pd (II) complexes were reported with 6-methyl-2-(phenyl(p-tolylamino)methyl)-4H-furo[3,2-c]pyran-3,4(2H)-dione derived ligands. [MLCl2] is the proposed general formula of complexes. The synthesized complexes were prepared and experimentally characterized by elemental analysis, SEM-EDX, FTIR, 1HNMR, UV-Visible spectroscopy, magnetic measurement, conductivity and thermal analysis. FTIR spectroscopy has revealed the coordination mode of complexes through nitrogen and oxygen atoms. The Density Functional Theory calculation was applied to optimize the geometric structure of complexes, whereas Orbital Molecular Frontiers calculations allowed to define their stability. The experimental electronic spectra and magnetic measurement results approved a square planar geometry for all complexes. These results correlate with theoretical calculations. The electrochemical behaviour of ligands and complexes were determined by cyclic voltammetry, which reveal different redox processes of Pd (II) couples for the three complexes. The antimicrobial activity of all compounds was evaluated against different micro-organisms using agar disc-diffusion method. Most of compounds exhibit a remarkable inhibition against standard yeasts and S. aureus Gram positive bacteria. The ADMET study was carried out to predict pharmacokinetic and toxicity of synthesised compounds. Molecular Docking of HL3 and Pd-HL3 against S. aureus was applied in order to study their molecular interactions
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    Algerian wild fennel essential oils: chromatographic profile, acute toxicity, antioxidant, and antimicrobial activities
    (Springer, 2022) Dahman, Karima; Moghrani, Houria; Deghbar, Nahla; Ouare, Salima; Alla, Karim; Arab, Karim
    The present study deals with the characterization of essential oils from umbels and seeds of Algerian wild (bitter) fennel (Foeniculum vulgare Mill. Var vulgare) by determining the chromatographic profile, lethal dose (LD50), antioxidant and antimicrobial activities, as well as a kinetic modeling study of the extraction of the seed-based essential oils. The extrac- tion of essential oils (EOs) was performed by hydrodistillation using Clevenger for 3.5 and 6 h for the umbels and seeds, respectively. The two mathematical models from the experimental data show a good fit with an R2 of 99.99 and 98.94%. GC/ MS analyses of fennel EOs showed that fennel was rich in different oxygenated monoterpenes compounds. However, while fenchone was the main compound in fennel seeds (FSEO), fennel umbel EO (FUEO) mainly contained α-pinene, o-cymene, sylvestrene, fenchone, Endo-fenchyl acetate, and carvacrol. The acute toxicity study of FSEO showed a lethal dose (LD50) of 4.9085 ± 0.1213 g/kg body weight in mice. Based on the free radical scavenging method using BHT as a positive control, the IC50 values were 9.9658 ± 0.057 mg/mL and 0.4570 ± 0.0456 mg/mL for FSEO and BHT, respectively. The study of antimicrobial activity in two gram-negative bacteria: Echerichi coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), and one gram-positive bacterium: Bacilus subtilis (ATCC 6633), as well as two fungal strains: Candida albicans (ATCC 10,231), Saccaromyces cerevisiaes (ATCC 9763), revealed that the fungal strains were more susceptible to FSEO and showed a significant fungicidal effect. The results of this study highlight the high quality of Algerian wild fennel, and the possibility of recovering it for use in the pharmaceutical, cosmetic, and food industries
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    Synthesis, biological activities of chalcones and novel 4-acetylpyridine oximes, molecular docking of the synthesized products as acetylcholinesterase ligands
    (Elsevier, 2022) Ould Lamara, Kamilia; Makhloufi-Chebli, Malika; Benazzouz-Touami, Amina; Terrachet-Bouaziz, Souhila; Robert, Anthony; Machado-Rodrigues, Carine; Behr, Jean-Bernard
    Heterocyclic chalcones were synthesized by reaction of 4-acetylpyridine with the corresponding aromatic aldehydes under Claisen Schmidt conditions. These chalcones were used as starting material for the synthesis of oximes in the presence of hydroxylamine hydrochloride. The structures of the synthesized compounds were confirmed by IR, 1H NMR, 13C NMR and ESI-MS, HRMS spectral analyses. All the synthesized compounds were evaluated for their antioxidant activity by DPPH• method and their in vitro antimicrobial activity by disk diffusion method against two Gram-negative bacteria, one Gram-positive bacteria and two fungal strains (C. albicans and A. niger). The results showed that the synthesized compounds did not display significant antioxidant activity. However, compounds 3b, 3d, 3f, 3h, 3i showed excellent antibacterial activity better than the standard drug against the bacterial strain S. aureus (ATCC 25923). The two compounds 3c, 3d proved very active against the fungal strain A. niger (MIC= 7.81 µg/ mL, 15.62 µg/mL respectively) while the antifungal drug used as reference (Fluconazole) was inactive. Molecular docking and molecular dynamics results revealed that the synthesized compounds, 4e, 4c, and 5j, were involved in a large number of favorable interactions with the active site residues of the acetylcholinesterase protein, which can stabilize the ligands in the active site and increase their affinities
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    Antimicrobial effect of different organic and aqueous extracts from a lamiaceae; thymus numidicus harvested in bouira (algeria)
    (2017) Behidj-benyounes, N.; Dahmane, T.; Boumghar, N.; Mekhazeni, I; Taki, L.
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    Phytochemical screening of Algerian borago officinalis L. and evaluation of its antioxidant and antimicrobial activities against respiratory pathogens
    (Advanced Research Journals, 2014) Afif Chaouche, Thanina; Arab, Karim; Bendahou, Mourad
    Context and purpose of the study: Infectious diseases represent a serious problem of public health in countries where resistance of bacteria to antibiotics is spreading alarmingly. Such situation leads researchers to make efforts so they can isolate or synthesize new antimicrobial molecules or molecules that inhibit the resistance mechanisms. Medicinal plants can represent a rich source of such molecules. In this context, Borago offccinaiis L, a medicinal species which is used traditionally in Algeria to heal infectious diseases of respiratory system is studied. Both of chemical composition and biological activities were explored. Main findings: GC-MS analysis of the essential oil reveals the Spathulenol as the most abundant component (38.24%). The HPLC applied on flavonoids extract shows the presence of the Caffeic acid, p-coumaric acid, Rutin, Rosmarinic acid, Quercetin and the Gallic acid. The test of the antioxidant capacity using the DPPH method reveal an inhibiting effect which is more important with the flavonoid extract with an EC 50 = 4.02± 0.06μg/ml. Otherwise, resistant strains to conventional antibiotics seem to be sensitive to the flavonoids with MICs varying from10.14μg/ml to 81.12μg/ml. Brief summary and potential implications: This study indicates that Borago offccinalss L. has an important antimicrobial effect, which justifies its potential use in infectious diseases. This species remains poorly investigated, further refined studies on its pure secondary metabolites are needed, in the perspective of identifying new antimicrobial molecules from this plant
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    Studies on the essential oil composition and antimicrobial activity of Thymus algeriensis Boiss
    (International Journal of Aromatherapy, 2006) Dob, T.; Dahmane, D.; Benabdelkader, T.; Chelghoum, C.
    Many species of Thymus have been widely used in Algerian folk medicine as astringent, expectorant and cicatrising agents. This study was designed to investigate for the first time the essential oil of the aerial parts of wild growing Thymus algeriensis endemic in North Africa. The volatile oil obtained by hydrodistillation was characterized by the physico-chemical properties, GC and GC/MS techniques. The yield of the oil was 1.13% (w/w), based on dry weight. Fifty-five components, representing 94.3% of the total oil, were identified. The oil was distinguished by its high content of oxygenated monoterpenes (79.5%) and was found to possess the following major components: linalool (47.3%), thymol (29.2%) and p-cymene (6.8%). Furthermore, the oil was tested for antimicrobial activity against four bacteria, two fungi and two yeasts. This oil exhibited a significant in vitro antimicrobial activity against Bacillus subtilis (MIC = 0.5 μL/mL), as well as against all yeast and all filamentous fungi tested (MIC = 0.5 and 1.0 μL/mL). © 2006 Elsevier Ltd. All rights reserved
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    Composition and antimicrobial activity of the essential oil of Thymus fontanesii
    (Pharmaceutical Biology, 2006) Dob, T.; Dahmane, D.; Benabdelkader, T.; Chelghoum, C.
    The yield of oil isolated by hydrodistillation from aerial parts of Thymus fontanesii Boiss. et Reut. (Lamiaceae) growing wild in Djelfa (Algeria) was 0.9%. Analysis of the oil by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) revealed the identification of 47 components accounting for 98.5% of the total oil. The oil was found to be rich in monoterpenes (91.5%) with major constituents being thymol (29.3%), γ-terpinene (21.7%), p-cymene (15.9%), and thymol methyl ether (11.4%), while a smaller amount of linalool (4.8%) and β-caryophyllene (2.9%) were detected. According to the antimicrobial study using the disk diffusion method and the agar dilution assay-minimal inhibitory concentration (MIC), the oil showed strong in vitro growth inhibition activity against Gram-negative bacteria and antifungal activity. The oil exhibited the maximum antifungal activity against Mucor ramaniamus (MIC = 0.2 μL/mL). © 2006 Informa Healthcare