Publications Internationales
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Item Antifungal and anti-toxigenic activities of Origanum onites and Thymus capitatus essential oils and ethanolic extracts against mycotoxigenic fungi isolated from barley(Elsevier, 2025) Dammak, Islem; Hamdi, Zohra; Lamine, Myriam; Hajri, Haifa; Basiouni, Shereen; Ntougias, Spyridon; Tsiamis, George; Yilmaz, Mete; Acheuk, Fatma; Emekci, MevlutWith the purpose of identifying biological substances for controlling Aspergillus-caused aflatoxin B1 and ochratoxin A contamination in cereals, particularly in barley, we assessed the efficiencies of Origanum onites and Thymus capitatus essential oils (EOs) and ethanolic extracts (EEs) under in vitro conditions. NMR and GC-TOF-MS analysis revealed the metabolite profiles with carvacrol being the major component in both EOs, and various terpenes, carbohydrates, phenols, flavonoids, and alcohols in the complex EEs. All tested EOs and EEs completely inhibited mycelial growth, sporulation, and mycotoxin production in vitro, albeit at different concentrations: O. onites EO displayed higher antifungal and anti-mycotoxigenic activities than T. capitatus EO. Notably, O. onites EO effectively protected barley grains from A. flavus, A. niger, and ochratoxin A and aflatoxin B1 contamination, during storage when applied via fumigation. Antioxidant activities of EEs were generally higher than those of EOs, with O. onites EE being the most potent antioxidant mixtureItem Phytochemical screening, antioxidant, antibacterial, and antifungal properties of the Cymbopogon citratus methanolic extract(Elsevier, 2025) Nacer, Salah Neghmouche; Wassima, Lakhdari; Boussebaa, Walid; Abadi, Abderrezzak; Benyahia, Ibtissem; Mouhoubi, Djawahir; Ladjal, Amel; Hammi, Hamida; Bachir, Hakim; Dehliz, AbderrahmeneIn this work, the aerial parts of Cymbopogon citratus, harvested in Algeria, were analyzed as potential sources of biologically active compounds. The phytochemical composition, the content of total phenols and flavonoids in the methanolic extract of this plant, as well as its antioxidant, antibacterial, and antifungal capacities, were evaluated. The methanolic extract had phenolic compounds and flavonoids, with levels of 92.82 µg GAE/mg Dry weight and 58.98 µg QE/mg Dry weight, respectively. Furthermore, the methanolic extract exhibited moderate antioxidant activity as assessed by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′azinobis (3-ethylbenzothiozoline-6-sulfonic acid) disodium salt radical), and β-carotene tests, while its antimicrobial activity was tested against four bacterial strains. Thus, a MBC/MIC ratio of 2 indicates that the extract exhibits bactericidal activity against Escherichia coli. Additionally, the extract exhibited antifungal activity against Thielaviopsis paradoxa, Alternaria sp., and Fusarium solani, with inhibition rates ranging from 50.59 % to 70.20 %. LC-MS analysis identified a variety of components, including chlorogenic acid (17.40 %), catechin (34.64 %), salicin (9.55 %), and curcumin (8.97 %), which are likely responsible for the observed activities. This study demonstrated that the aerial parts of Cymbopogon citratus offer a cost-effective source of natural compounds with bactericidal and antifungal activity, offering potential applications in the food and pharmaceutical industriesItem Chemical composition, antimicrobial and antioxidant activities of methanolic extracts of the Algerian Artemisia campestris L. at different stage of growth(Springer, 2024) Brahmi, Fairouz; Berrached, Rachda; Kebbouche Gana, Salima; Kadik, Leila; Lenchi, NesrineArtemisia campestris, widely abundant in North Africa, is usually used as a decoction to treat several diseases; but its chemical composition at different growth stages and along a climatic gradient has never been investigated. In this study, phytochemical content, antioxidant and antimicrobial activities of crude methanolic fractions from dry aerial parts of A. campestris, growing in two different bioclimatic conditions, were compared. The growth stages, vegetative and reproductive, were also taken into consideration. The HPLC analysis revealed the presence of several compounds like ascorbic, isovannilic, ferrulic and cinnapic acids. The antioxidant activity was evaluated with two different methods: DPPH and FRAP essay. The four samples showed very high antioxidant activity. The in vitro antimicrobial activity shows a strong inhibitory effect against gram positive bacteria. But, they were resistant to gram negative bacteria and yeast. The biggest inhibition diameter was recorded against S. aureus and B. cereus.Item Arisarum vulgare: Bridging Tradition and Science Through Phytochemical Characterization and Exploring Therapeutic Potential via In vitro, In vivo, and In silico Biological Activities(John Wiley & Sons, 2024) Bouafia, Zineb; Boudjelal, Amel; Terrachet, Souhila; Bouhenna, Mustapha Mounir; Bouchahdane, Souheila; Boulila, Abdennacer; Yıldız, Ilyas; Yılmaz, Sinem; Özen, Tevfik; Demirtas, IbrahimArisarum vulgare O. Targ. Tozz. (Araceae), locally known as “Elbgouga,” holds significant traditional importance in Algeria for the treatment of various human ailments, including pain, infections, inflammation, digestive disorders, cancer, skin problems, eczema, wounds, and burns. The aim of this study was to explore for the first time the phytochemical profile, antioxidant, antibacterial, and inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, DNA protection, and capacity to promote wound healing. Preliminary phytochemical experiments were conducted to evaluate the major classes of bioactive compounds, in addition to the total phenol and flavonoid amounts in AVEE. An LC-MS/MS analysis was conducted to clarify the phytochemical composition of this particular botanical species. The antioxidant capacity was assessed using DPPH· and ABTS·+ radical scavenging tests. The agar diffusion approach was used to ascertain the antibacterial efficacy against four bacteria (Staphylococcus aureus, Enterococcus faecium, Escherichia coli, and Salmonella typhimurium). Cholinesterase inhibition was evaluated using a colorimetric method that relies on Ellman's reaction. The protective effects of AVEE on pBR322 plasmid DNA damaged by H2O2 and UV treatment were assessed by their DNA-breaking forms. The in vivo acute dermal toxicity and wound healing potential of the AVEE ointment (1–5% AVEO) were also investigated, and histological analyses were carried out on biological samples. Five protein targets were the subject of an in silico molecular docking investigation (TNFα, TGFBR1, IL-1ß, GSK-3ß, and NOS) and ADMET studies of the main components of the extracts were performed. The screening of phytochemicals revealed a significant concentration of phenolic compounds (176.00 ± 1.08 mg of gallic acid equivalents/g of dry extract), mainly flavonoids (81.21 ± 1.24 mg of quercetin equivalents/g of dry extract). Three major compounds were identified by LC-MS/MS analysis, belonging mainly to the class of flavonoids, with rutin as the most abundant compound (11.85 mg/g extract), followed by hesperidin (4.125 mg/g extract). A conspicuous presence of isoquercitrin (1.327 mg/g extract) was also found. Small amounts of flavonoids and phenolic acids were also detected (catechin, chlorogenic acid, syringic acid, salicylic acid, kaempferol, and luteolin). DPPH assay showed higher antioxidant capacity compared to the ABTS·+ assay (142.60 ± 5.52 µg/mL and 236.10 ± 0.22 µg/mL, respectively). AVEE was effective against all selected bacterial strains; however, the highest zone of inhibition (36.00 ± 0.1 mm) was noted against E. faecium. The extract of the plant significantly inhibited both AChE and BChE (89.98 ± 18.76 µg/mL inhibitory activity against AChE and 98.28 ± 44.68 µg/mL inhibitory activity against BChE, respectively). The ethanolic extract of A. vulgare and quercetin exhibited more significant DNA protection action in form I, with percentages of 90.41 and 94.23%, respectively, compared to form II, where the percentages were 27.91 and 51.92%, respectively. The AVEO formulation may be safely administered by topical application. A statistically significant wound contraction was discovered in the group treated with 5% AVEO compared to the groups that were not treated or treated with petroleum jelly. Furthermore, there was no notable disparity detected between the group treated with 5% AVEO and the group treated with the reference drug. The 5% AVEO-treated group showed, after the research was complete, the most significant percentage of wound contraction (96.90 ± 0.42%). Moreover, the topical application of the formulation improved histological parameters. In silico study showed that rutin, hesperidin, and isoquercitrin had a high affinity to the five main targets that might contribute to the wound healing potential of A. vulgare ethanolic extract. The impressive biological capabilities of A. vulgare indicate that the plant has the ability to be a valuable source of bioactive chemicals with various medical applications.Item Comparative analysis of the chemical constituents and in vitro antioxidant activities of different aqueous extracts of cistanche phelypaea (L.) Cout. from Algeria(Elsevier, 2022) Rahim, Meriem Zohra; Prinsi, Bhakti; Espen, Luca; Benabdelkader, Tarek; Nabiev, Mohamed; Houali, KarimCistanches herba are well-known for their nutritional and therapeutic effects, but only few reports are available about the species Cistanche phelypaea (L.) Cout. and studies of aqueous extracts are scarce. This study aimed to elucidate the impact of aqueous extraction methods including decoction, infusion and cold maceration from C. phelypaea roots on phytochemical composition and in vitro antioxidant activities of the extracts. In order to accomplish this, determinations of total phenolic and flavonoid contents, liquid chromatography electrospray ionization mass spectrometry (LC/ESI-MS and LC/ESI-MS/MS) analyses and in vitro antioxidant activity studies were carried out. All extracts had significant levels of total phenolic content, but the decoction possessed the highest concentration and no significant difference was observed between infusion and cold maceration. Concerning flavonoids, decoction and infusion possessed the highest concentrations. Data obtained from LC/ESI-MS and LC/ESI-MS/MS analyses showed similar qualitative profiles for all aqueous extracts with 14 characteristic chromatographic peaks in negative mode and 6 in positive mode, which allowed the tentative identification of syringin, 14 phenylethanoid glycosides and 5 iridoids, including 5 pairs of isomers. However, significant variations were recorded for the relative abundance of some compounds depending on the method that was employed. Hot extraction procedures, decoction and/or infusion were more efficient in extracting acteoside, isoacteoside and two molecules corresponding to isomers of 2ʹ-acetylacteoside/tubuloside B, and it seems that a longer time of heating-extraction was required for an improved extraction of isoacteoside, and one of the two isomers of 2ʹ-acetylacteoside/tubuloside B, as decoction gave a significant higher amount of these compounds. However, 8-epiloganic acid and cistanoside F, with their respective isomers, and syringin were more extractable by the cold maceration process. All extracts had similar antioxidant properties in scavenging DPPH radical and total antioxidant activity assays, with the exception of the ferric-reducing power activity assay, in which cold maceration exhibited a significantly less potent activity. Aqueous extracts of C. phelypaea roots present an interesting antioxidant potential which is related to the synergistic effects of several antioxidant compoundsItem Design, Synthesis, Biological evaluation of Isonicotinoyl-pyrazolyl-coumarin derivatives and computational study(Elsevier, 2022) Halit, Sabrina; Benazzouz-Touami, Amina; Makhloufi-Chebli, Malika; TerrachetBouaziz, Souhila; Ighilahriz, Karima; Robert, Anthony; Machado-Rodrigues, CarineA new series of 3-(5-Hydroxy-1-isonicotinoyl-3-methyl-4,5-dihydro-1H-pyrazol-5-yl)-2H-chromen-2-one derivatives 3a-g was efficiently synthesized under environmentally friendly reaction conditions by reaction of 3-acetoacetylcoumarin derivatives 1a-g with isoniazid 2. The reaction is conveniently performed at room temperature using Knorr pyrazole syntheses. The key features of this approach are its operational simplicity, the facile access to the desired products, and the good to excellent yields. The structures of the newly synthesized compounds were established by 1H, 13C, 2D NMR spectroscopy and mass spectrometry. 1H-15N HMBC experiments were also conducted to assess the regiochemistry of final compounds. All the synthesized compounds were evaluated for their in vitro antifungal activity against both Candida albicans and Aspergillus niger strains and compared to the standard drug Ketoconazole. Among all the compounds, 3d and 3e were the most effective, showing high potential antigungal inhibitory activities with respective MIC values of 15.62 µg ml−1 for 3e against Aspergillus niger. All the newly synthesized derivatives 3a-g are evaluated for their antioxidant activity and showed good activity. SAR studies demonstrated that the presence of nitro and hydroxyl groups in the aromatic ring of the coumarin and pyrazoline rings conferred enhanced antioxidant and antifungal activity. In addition, molecular docking studies were performed for compound 3e to evaluate its potential as an inhibitor of the fungal protein (Phytase A) from Aspergillus niger and the results suggested that our isonicotinoyl-pyrazolyl-coumarin may act as promising antifungal agentItem Sodium dodecyl benzene sulfonate-catalyzed reaction for green synthesis of biologically active benzylpyrazolyl-coumarin derivatives, mechanism studies, theoretical calculations(Elsevier, 2022) Halit, Sabrina; Benazzouz-Touami, Amina; Makhloufi-Chebli, Malika; Terrachet-Bouaziz, Souhila; Ighil Ahriz, KarimaIn the present work, a multicomponent protocol has been described for the synthesis of various benzylpyrazolyl-coumarin 5a-i structures, involving the reaction of 4-hydroxycoumarin, ethyl acetoacetate, hydrazine hydrate, and aldehydes, using an ethanol-water (1:1) mixture as the reaction medium and sodium dodecylbenzenesulfonate (SDBS) as the catalyst. This new approach has advantages such as short reaction time, recovery of the catalysts after the catalytic reaction and their reuse without loss of activity, as well as the lightening of the process. The structures of the obtained benzylpyrazolylcoumarins were determined and confirmed by melting point, 1H NMR and 13C NMR. The determination of antioxidant activity of the obtained benzylpyrazolylcoumarin 5a-i derivatives was studied using the DPPH scavenging assay. Molecular modeling studies using DFT (density function theory) calculations showed that there are six tautomeric structures A to F in which structure C is more stable than the othersItem Synthesis, biological activities and molecular docking study of 3-(3-oxobutanoyl)-2H-chromen-2-one derivative(Elsevier, 2021) Benazzouz-Touami, Amina; Hikem-Oukacha, Djamila; Ould Lamara, Kamilia; Halit, Sabrina; Terrachet-Bouaziz, Souhila; Makhloufi-Chebli, MalikaHerein, we report the highly efficient total synthesis of a series of 3-(3-oxobutanoyl)-2H-chromen-2-one derivatives from salicylaldehyde(s) and 4‑hydroxy-6-methyl-2H-pyran-2-one (TAL) using Na2S2O3 as green and efficient catalyst. Structure elucidation of the products has been accomplished based on FT-IR, mass spectroscopy, 1H NMR and 13C NMR spectroscopy. The in vitro antioxidant activities of the drugs 3-(3-oxobutanoyl)-2H-chromen-2-one were tested by the quantitative 1,1-diphenyl-2-picrylhydrazyl (DPPH.) radical scavenging activity method. The compounds 3b, 3c and 3e proved to be the most active, showing high capacity to deplete the DPPH radicals. All synthesized compounds were screened for their antimicrobial activities and the results show that only 3f was active against bacteria Staphylococcus aureus compared to antibiotic used as reference. In addition, all synthesized compounds were docked with FtsZ from S. aureus protein using AutoDock 4 software. The results suggest that 3f binds with FtsZ protein with lower energy and undergoes good interactions with the active site aminoItem Selectivity control in the reaction between 2-hydroxyarylaldehydes and 4-hydroxycoumarin. Antioxidant activities and computational studies of the formed products(Elsevier, 2021) Ould Lamara, Kamilia; Makhloufi-Chebli, Malika; Benazzouz-Touami, Amina; Terrachet-Bouaziz, Souhila; Hamdi, Nejla; Silva, Artur M.S.; Behr, Jean-BernardA series of 6H,7H-7-(4-Hydroxy-3-coumarinyl)[1]benzopyrano[4,3-b][1]benzopyran-6-ones 4a-g and 3-(2-hydroxybenzoyl)-2H-chromen-2-ones 5a-g derivatives were synthesized by reaction of 4-hydroxycoumarin with 2-hydroxyarylaldehydes 2a-f or 2-hydroxynaphtaldehyde 2g using different solvents and acid/base catalysts. The approach relies on a regioselective cascade reaction involving one/two molar equiv of the 4-hydroxy coumarin iteratively acting as active methylene substrate in a Knoevenagel condensation and in a Michael addition. The structures of all compounds were established by IR, mass spectrometry, 1H-NMR and 13C-NMR. Antioxidant activity of the synthesized compounds were determined using the DPPH scavenging assay, best results being obtained with 5b (IC50 = 236 µg/mL). Computational studies showed that the compounds bind in the ATP-binding site of p38 MAPK, in a same manner than known polyaromatic potent inhibitors. The synthesized compounds might be considered further for cancer therapyItem Evaluation of some biological activities of phenolic compounds obtained from two Algerian medicinal plants : mentha rotundifolia and satureja calamintha(2020) Benfares, Redhouane; Boudjema, Khaled; Behlali, Hadjira; Imedjdouben, Imene; Kennas, Abderrezak; Fazouane, Fethia; Jaroslava, Švarc-GajićIn this work phytochemical characterization of two medicinal plants from Lamiaceae family, Mentha rotundifolia and Satureja calamintha, has been carried out. Extracts obtained with different solvents were screened for different plant secondary metabolites and were biologically characterized by defining their antiradical and antibacterial activi-ties. Phytochemical screening of M. rotundifolia and S. calamintha confirmed their rich-ness in different secondary metabolites. The determination of phenolic compounds revea-led high polyphenols contents in water: methanol (30:70) extracts with concentrations of 20.64±1.74 mg EAG/g DW and 13.45±0.91 mg EAG/g DW for M. rotundifolia and S. calamintha, respectively. These extracts were also characterized by high concentrations of flavonoids (Mentha rotundifolia 12.33±1.58 mg EQ/g DW, Satureja calamintha 7.11±0.02 mg EQ/g DW). Furthermore, the water:methanol (30:70) extract of M. rotun-difolia was the most effective in inhibiting free radicals. Recorded inhibition diameters for both plant samples and tested microbial strains ranged from 6.66 mm to 13.66 mm. Presented results confirmed that tested indigenous Algerian plants are favorable sources of polyphenols with antioxidant and antimicrobial properties