Publications Internationales
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Item Enhancing agriculture recovery of Phaseolus vulgaris L. and Cucurbita pepo L. with Olea europaea L. plant growth promoting rhizobacteria(CSIRO, 2022) Hadjouti, Rima; Mohand Kaci, H.; Benzina, F.; Furze, J.N.The rhizosphere is an environment created by interactions between root exudates and microorganisms. Interactions are beneficial due to certain components having a plant growth promoting rhizobacteria (PGPR) effect. Aims. This study consists of the isolation, screening of PGPR from the rhizosphere of Olea europaea L. of a Mediterranean climatic region in Algeria and the study of their effects on growth of two agronomic vegetables Phaseolus vulgaris L. and Cucurbita pepo L. Methods. Based on their ability to produce the PGPR molecules indole-3- acetic acid (IAA), phosphatase and siderophores, three rhizobacteria (S25, S75, and S79) were chosen for in vivo tests and capacity to produce the cell wall degrading enzymes chitinase, lipase, protease, glucanase, cellulase, and and phospholipase. They were also examined using scanning electron microscopy (SEM) and analysed using matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI-TOF MS) for identification. Key results. Bacterial strains identified as Bacillus cereus and Bacillus thuringiensis were able to enhance significantly germination of the two vegetables at P < 0.001. Vegetative parameters of C. pepo were significantly affected by the bacterial inoculation. We noted increases in stem length (P < 0.05), number of flowers (P < 0.01), and root length (P < 0.001). Conclusion. The bacterial isolates of this study provide biological options in treatments originating from alternate hosts. Implications. They provide hope for companion/intercrop planting schemes, leading to optimisation of agricultural yields in agroecological blends.Item Antagonistic activity of Bacillus sp. obtained from an Algerian oilfield and chemical biocide THPS against sulfate-reducing bacteria consortium inducing corrosion in the oil industry(2010) Gana, M.L.; Kebbouche-Gana, Salima; Touzi, A.; Zorgani, M.A.; Pauss, A.; Lounici, H.; Mameri, N.The present study enlightens the role of the antagonistic potential of nonpathogenic strain B21 againstsulfate-reducing bacteria (SRB) consortium. The inhibitor effects of strain B21 were compared with those of the chemical biocide tetrakishydroxymethylphosphonium sul- fate (THPS), generally used in the petroleum industry. The biological inhibitor exhibited much better and effective performance. Growth of SRB in coculture with bacteria strain B21 antagonist exhibited decline in SRB growth, reduction in production of sulfides, with consumption of sulfate. The observed effect seems more important in comparison with the effect caused by the tested biocide (THPS). Strain B21, a dominant facultative aerobic spe- cies, has salt growth requirement always above 5% (w/v) salts with optimal concentration of 10–25%. Phylogenetic analysis based on partial 16S rRNA gene sequences showed that strain B21 is a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis DQ115802 (94.0% sequence similarity), Bacillus aiding- ensis DQ504377 (94.0%), and Bacillus salarius AY667494 (92.2%). Comparative analysis of partial 16S rRNA gene sequence data plus physiological, biochemical, and phe-notypic features of the novel isolate and related species of Bacillus indicated that strain B21 may represent a novel species within the genus Bacillus, named Bacillus sp. (EMBL, FR671419). The results of this study indicate the application potential of Bacillus strain B21 as a biocontrol agent to fight corrosion in the oil industry
