Doctorat

Permanent URI for this collectionhttps://dspace.univ-boumerdes.dz/handle/123456789/19

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Author: search.filters.author.Bouchenak, Ouahiba(Directeur de thèse)

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    Extraction methods of biologically active compounds of essential oil from marjoram (origanum majorana) and their applications in pharmaceutical formulation
    (Université M'Hamed Bougara Boumerdès : Faculté des Sciences, 2025) Hafid, Nourehouda; Bouchenak, Ouahiba(Directeur de thèse)
    Objective: The main objective of the present work is to perform the phytochemical study investigating some biological and therapeutic properties such as antioxidant, antimicrobial, anticancer effects of Origanum majorana’s essential oil (OMEO), and its toxicity with wound healing properties in animals. Materials and Methods: An ethnobotanical study is conducted on Origanum majorana in the local population of Boumerdes. the essential oil was obtained using hydro-distillation technique with; later, it is subjected to chemical analysis using GC-MS. Assays of OMEO’s antioxidant activity by DPPH, and ABTS were carried out. The antibacterial activity is determined by disc diffusion and minimum inhibitory concentration techniques against different bacterial and fungal types. The anticancer activities are performed by MTT assays, Apoptosis assay, and migration assay, on PC-3 and SKBR3 cancer cell lines. In vivo toxicity is carried out on animal models, and the wound healing effects of a developed cream are studied using a cream formulation on induced wounds. Results: The ethnobotanical survey revealed that in 500 individuals questioned only 21% knew and used Origanum majroana. Regarding the essential oil’s hydrodistillation extraction yielded 3.33%. The analysis by GC-MS , revealed 29 compounds, which represent 98.08% of total volatile oil. The major compounds identified in OMEO were terpinen-4-ol (21.37%), ?-terpinene (15.78%), a-terpinene (10.43%), and trans-sabinene hydrate (9.27%). The assessement of the antioxidant activity showed different reactions to free radical scavenging activity with IC50 of 11.7mg/ml , and 265.2µg/ml, for DPPH , and ABTS, respectively, compared to BHT that exhibited an important activity with IC50 of 2.85µg/ml, and, 11.19µg/ml, respectively for DPPH and ABTS. Furthermore, The oil expresses a notable antimicrobial effect against most of the tested strains, the bacteria exhibit inhibition zones ranging from 10 to 31 mm and IC50 values from ?15.6 to 1051.9 ?g/ml for bacteria. Regarding the antifungal activity, the minimal inhibitory concentration (MIC) of the oil shows good results for Malassezia furfur, Candida albicans, Candida glabrata, Trichosporon sp., and Aspergillus niger, ranging from 0.125 to 0.250 ?l/ml. In the anticancer activity, the cytotoxicity evaluated by MTT on prostate cancer (PC-3), breast cancer (SKBR3), and normal retinal pigment epithelium (ARPE19) cell lines. The results show a selective cytotoxicity effect, by decreasing cell viability of PC-3 cancer cells with half Inhibitory Concentration (IC50) of 608.57µg/ml and 672.5µg/ml after 48h and 72h respectively. Regarding SKBR3 cancer cells the IC50 was 991.5µg/ml. OMEO exhibites no significant cytotoxicity against normal (ARPE19) cells. Furthermore, we conducted a cell apoptosis assay using Hochest 33342 dye to explore the potential mechanism pathway of OMEO. The findings verifie that OMEO could trigger apoptosis in PC3 and SBKR3 cancer cells. The ability of OMEO to inhibit cell migration assessed via wound healing assay reveals a significant decrease in cell migration. Acute toxicity study in vivo proves a moderate toxicity of 2500g/kg of the essential oil for therapeutic doses. Moreover, the test of wound healing reveals that 1% cream shows the most potent effect in tissue regeneration among the treated groups. Conclusion: The present study opens a window on the promising therapeutic potentials of Origanum majorana essential oil as a natural antioxidant, antimicrobial, and anticancer agent. Its pharmaceutical value is increasing by selective cytotoxicity and wound healing potential. However, additional studies need to be carried out to understand the mechanisms of action and explore possible clinical applications
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    Caractérisation phytochimique et évaluation des propriétés pharmacologiques in vivo et in vitro des extraits de deux plantes médicinales Algérienne : formulation d’une forme
    (Université M'Hamed Bougara Boumerdès : Faculté des Sciences, 2024) Rezzoug, Hamida; Bouchenak, Ouahiba(Directeur de thèse)
    Cette étude vise à caractériser la composition chimique et d'évaluer la cytotoxicité et la bioactivité des huiles essentielles (HE) de Melissa officinalis (MO) et de Lavandula stoechas (LS) et l’effet synergique contre C. albicans afin de formuler des crèmes antifongiques. La composition chimique est analysée par GC-FID et GC/MS. Les interactions entre les deux huiles essentielles, ainsi que la cytotoxicité et les composés phénoliques, sont évalués. Les activités antioxydantes (par DPPH et ABTS•+), antibactériennes (sur 12 souches) et antifongiques (sur 9 souches) sont étudiées. Des formulations anti-candidose sont testées in vitro et in vivo contre Candida albicans. Les résultats montrent que les principaux composés de l'huile essentielle de MO sont le géranial (35,7 %) et le néral (24,1 %), tandis que le 1,8-cinéole (61,9 %) domine dans l'HE de LS. Ces deux huiles essentielles présentent une activité antioxydante plus importante contre les radicaux ABTS•+ que contre DPPH et une activité antibactérienne plus efficace contre les bactéries Gram+ que Gram-. De plus, une action antifongique substantielle contre tous les micro-organismes testés avec des valeurs de CMI allant de 0,125 à 0,0019 des HE de MO. L'huile essentielle combinée avec celle de L. stoechas montre également un effet anti- candidose significatif. Les crèmes formulées montrent un effet hautement significatif contre C. albicans in vitro et in vivo. Ces résultats suggèrent les bonnes qualités bioactives des crèmes topiques développées contre C. albicans, révélant leur potentiel pour être éventuellement appliquées comme agent antifongique en pharmacie industrielle