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    Chemical composition, antimicrobial and antioxidant activities of methanolic extracts of the Algerian Artemisia campestris L. at different stage of growth
    (Springer, 2024) Brahmi, Fairouz; Berrached, Rachda; Kebbouche Gana, Salima; Kadik, Leila; Lenchi, Nesrine
    Artemisia campestris, widely abundant in North Africa, is usually used as a decoction to treat several diseases; but its chemical composition at different growth stages and along a climatic gradient has never been investigated. In this study, phytochemical content, antioxidant and antimicrobial activities of crude methanolic fractions from dry aerial parts of A. campestris, growing in two different bioclimatic conditions, were compared. The growth stages, vegetative and reproductive, were also taken into consideration. The HPLC analysis revealed the presence of several compounds like ascorbic, isovannilic, ferrulic and cinnapic acids. The antioxidant activity was evaluated with two different methods: DPPH and FRAP essay. The four samples showed very high antioxidant activity. The in vitro antimicrobial activity shows a strong inhibitory effect against gram positive bacteria. But, they were resistant to gram negative bacteria and yeast. The biggest inhibition diameter was recorded against S. aureus and B. cereus.
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    Evaluation of some biological activities of phenolic compounds obtained from two Algerian medicinal plants : mentha rotundifolia and satureja calamintha
    (2020) Benfares, Redhouane; Boudjema, Khaled; Behlali, Hadjira; Imedjdouben, Imene; Kennas, Abderrezak; Fazouane, Fethia; Jaroslava, Švarc-Gajić
    In this work phytochemical characterization of two medicinal plants from Lamiaceae family, Mentha rotundifolia and Satureja calamintha, has been carried out. Extracts obtained with different solvents were screened for different plant secondary metabolites and were biologically characterized by defining their antiradical and antibacterial activi-ties. Phytochemical screening of M. rotundifolia and S. calamintha confirmed their rich-ness in different secondary metabolites. The determination of phenolic compounds revea-led high polyphenols contents in water: methanol (30:70) extracts with concentrations of 20.64±1.74 mg EAG/g DW and 13.45±0.91 mg EAG/g DW for M. rotundifolia and S. calamintha, respectively. These extracts were also characterized by high concentrations of flavonoids (Mentha rotundifolia 12.33±1.58 mg EQ/g DW, Satureja calamintha 7.11±0.02 mg EQ/g DW). Furthermore, the water:methanol (30:70) extract of M. rotun-difolia was the most effective in inhibiting free radicals. Recorded inhibition diameters for both plant samples and tested microbial strains ranged from 6.66 mm to 13.66 mm. Presented results confirmed that tested indigenous Algerian plants are favorable sources of polyphenols with antioxidant and antimicrobial properties
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    Comparison of five solvents in the extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel
    (Najfnr, 2019) Abderrezak KENNAS
    Pomegranate peels are rich in bioactive compounds and could be an alternative natural source such as antioxidants. Aims: The aim of the present study was to assess the abilities of five solvents to extract phenolic antioxidants from pomegranate peels. Methods and Material: Pomegranate peels powder was subjected to extraction and the extraction yield was compared. The total phenolic, flavonoid, condensed and hydrolysable tannins contents were analyzed. The antioxidant activity of the extracts was evaluated by two methods (DPPH* and ABTS•+ scavenging capacities) and results were then compared. Results: Results showed that the mixture methanol/water (50:50) allowed higher extraction yield (37.33±5.3%) than the others solvents (P<0.05). Further, the total phenolic, flavonoid and condensed tannins contents were the highest in mixture water/methanol (50:50) extract. Phenolic antioxidants showed a distinct reducing capacity and a high DPPH* inhibition values were recorded for all extracts with no significant differences (P>0.05) between ethanol and mixture water/methanol (50:50) extracts. All extracts exhibited high inhibition against ABTS•+ but with a considerable variation. Phenolic content and antioxidant activities were well positively correlated with each other. Conclusions: Our findings revealed that the choice of the extracting solvent affects considerably the extraction of phenolic antioxidants from pomegranate peels.
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    SENSORY ACCEPTANCE, QUALITY CHARACTERISTICS AND ANTIOXIDANT ACTIVITY OF YOGHURT FORTIFIED WITH HONEY AND POMEGRANATE PEEL
    (AFST, 2019) KENNAS, Abderrezak; AMELLAL-CHIBANE, Hayat
    Pomegranate (Punica granatum L.) peels and honey are rich in bioactive compounds and are promising as natural ingredients for functional product development. This study aimed to develop a novel yoghurt fortified with pomegranate peel and honey, and to investigate their effect on the sensory, physicochemical, textural, microbiological, and antioxidant properties throughout storage period (28 days). Fortification of yoghurt with 5 % of honey and 0.5 % of pomegranate peel powder showed a positive effect on several determinative properties such assyneresis, water-holding capacity, color, instrumental texture and sensory attributes. After the whole storage period, fortified yoghurt presented the same count of total lactic acid bacteria (Streptococcus thermophilus and Lactobacillus bulgaricus) as the control yoghurt that remained over 107 CFU/g. In addition, fortified yoghurt contained more polyphenols (nearly 7 fold higher) and displayed significantly (p<0.05) higher antiradical activity (DPPH and ABTS radical scavenging activity) than control yoghurt. In conclusion, fortification of yoghurt with 5 % of honey and 0.5 % pomegranate peel powder offered a novel yoghurt with acceptable sensory characteristics, good physicochemical and textural properties, and interesting antioxidant activity without inhibiting the development of lactic acid bacteria.
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    Effect of pomegranate peel and honey fortification on physicochemical, physical, microbiological and antioxidant properties of yoghurt powder
    (King Saoud University, 2020) Kennas, Abderrezak; Amellal-Chibane, Hayat; Kessal, Fetta; Halladj, Fatima
    Consumers are focusing more and more on health benefits of functional products. The aim of this work is to formulate and characterize novel functional powder obtained by freeze-drying yoghurt supplemented concomitantly with pomegranate peel powder (0–10%) and honey (0–5%). In order to examine the powdered yoghurt, it was subjected to measurements of physicochemical, physical, microbiological and antioxidant properties. The experimental data of freeze-drying were fitted to four models and results showed that Page model was the most suitable thin-layer drying model according to R2 and RMSE as statistical comparison indexes. There was no significant difference (p > 0.05) among the powder samples in terms of moisture content and flow properties. The bulk and tapped densities values of yoghurt samples were slightly affected (p < 0.05) by the addition of pomegranate peel and honey. The results also demonstrated that powders particles exhibit an amorphous structure with mean diameter (D4.3) values that varied from 106.68 to 143.37 lm. Moreover, the addition of pomegranate peel and honey increased the glass transition temperature (Tg) value of yoghurt powder, and this could improve its stability during storage. Total lactic acid bacteria in all yoghurt powders remained over 107 cfu/g of dry matter after the freeze-drying. All the fortified yoghurt displayed higher phenolic contents and, consequently, exhibited higher antioxidant activities than control powder sample. It is concluded that, the freeze-drying of yoghurt with pomegranate peel and honey, at the fortification levels tested, is feasible, and the obtained powder has acceptable physicochemical and physical properties which could improve its shelf-life. Thus, the formulated yoghurt powder could be considered as a potential functional powder with antioxidant properties.
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    Effect of maturation degree on the fixed oil chemical composition, phenolic compounds, mineral nutrients and antioxidant properties of pistacia lentiscus L. Fruits
    (2019) Boudieb, Kaissa; Ait Slimane-Ait Kaki, Sabrina; Amellal-Chibane, Hayet
    This study aimed to evaluate the maturity stage effects on the bioactive metabolites content, the antioxidant activity and color variation of Algerian Pistacia lentiscus L. fruit. For this propose, both red and black fruits of the selected species representing two different maturation stages immature and mature, respectively are chosen. Our experimental study was initiated by a GC/MS analysis in order to determinate the fixed oils chemical composition. An AAS for macroelements and ICP-MS for microelements was carried out to evaluate the mineral composition. Furthermore, phenolic, anthocyanins and total sugars compounds level were determinate. Also, a chromameter was used to detect the color changes of fruits powders. At last, antioxidant activities were evaluated by DPPH and FRAP assays. As a result, the fixed oil qualitative evaluation, revealed new molecules were synthesized in the mature fruit oil which gives it a higher quality than one of the immature stage, we also noted that the accumulation of the oils increases with the fruits maturation while the sugar content decreases significantly. In addition, the results obtained showed that the bioactive molecules were found to be significantly high at the mature stage and its affects positively the P. lentiscus antioxidant activity. High levels of minerals (Ca, Mg, Mn, Cu and Zn) observed in mature fruits ensure a high nutritional quality compared to immature ones. To conclude, the phytocomponents of P. lentiscus fruit are accumulated during maturation, which provides it with an important qualitative and quantitative value in terms of both curative and/ or nutritional fields
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    HPLC-UV/DAD and ESI-MSn analysis of flavonoids and antioxidant activity of an Algerian medicinal plant : paronychia argentea lam
    (Elsevier, 2015) Sait, Sabrina; Hamri-Zeghichi, Sabrina; Boulekbache-Makhlouf, Lila; Madani, Khodir; Rigou, Peggy; Brighenti, Virginia; Prencipe, Francesco Pio; Benvenuti, Stefania; Pellati, Federica
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    Phytochemical screening of Algerian borago officinalis L. and evaluation of its antioxidant and antimicrobial activities against respiratory pathogens
    (Advanced Research Journals, 2014) Afif Chaouche, Thanina; Arab, Karim; Bendahou, Mourad
    Context and purpose of the study: Infectious diseases represent a serious problem of public health in countries where resistance of bacteria to antibiotics is spreading alarmingly. Such situation leads researchers to make efforts so they can isolate or synthesize new antimicrobial molecules or molecules that inhibit the resistance mechanisms. Medicinal plants can represent a rich source of such molecules. In this context, Borago offccinaiis L, a medicinal species which is used traditionally in Algeria to heal infectious diseases of respiratory system is studied. Both of chemical composition and biological activities were explored. Main findings: GC-MS analysis of the essential oil reveals the Spathulenol as the most abundant component (38.24%). The HPLC applied on flavonoids extract shows the presence of the Caffeic acid, p-coumaric acid, Rutin, Rosmarinic acid, Quercetin and the Gallic acid. The test of the antioxidant capacity using the DPPH method reveal an inhibiting effect which is more important with the flavonoid extract with an EC 50 = 4.02± 0.06μg/ml. Otherwise, resistant strains to conventional antibiotics seem to be sensitive to the flavonoids with MICs varying from10.14μg/ml to 81.12μg/ml. Brief summary and potential implications: This study indicates that Borago offccinalss L. has an important antimicrobial effect, which justifies its potential use in infectious diseases. This species remains poorly investigated, further refined studies on its pure secondary metabolites are needed, in the perspective of identifying new antimicrobial molecules from this plant
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    Some physicochemical and morphological characterizations of three varieties of Algerian common dates
    (European Journal of Scientific Research, 2007) Chibane, H.; Benamara, S.; Noui, Y.; Djouab, A.
    The date palm is a typical plant of the arid and semi arid areas. Unfortunately in fact they are not all the varieties that profits from an economic interest except for Deglet-Nour. Thus, it represents a danger to the genetic inheritance and the biodiversity. The three varieties studied in this work are: Mech-Degla, Degla-Beida and Frezza, which are common varieties cultivated in the south of Algeria. They are characterized by a good availability and an interesting aptitude for the conservation. In this work (included in our project consisting in the valorization of the common local dates) were presented only the obtained first results. The morphological characteristics (weight of whole date, pea of pulp (pulpit), weight of the seed, length and width of whole date and the seed, pulp/seed ratio, pulp/date ratio, seed/date ration) are given. The physicochemical parameters studied are: pH, ashes, flavonoid, minerals and antioxidant activity. All three varieties are particularly rich in minerals (K, Ca, Na, Zn, Fe, Cu and Mn), in flavonoïd. Concerning the antioxidant activity, different cultivars is endowed with an extraordinary capacity (61.82, 61.56 and 61.61% respectively for Mech-Degla, Degla-Beida and Frezza). © EuroJournals Publishing, Inc. 2007